Aptamer selection and applications for breast cancer diagnostics and therapy

Liu, Mei; Yu, Xindi; Zhu, Cairong; Yang, Tong; Yang, Dandan; Liu, Qianqian; Du, Keke; Li, Bo; Wang, Zhifei; Song, Li; Deng, Yan; He, Nongyue

doi:10.1186/s12951-017-0311-4

Cited by 111 publications

(56 citation statements)

References 140 publications

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“…Furthermore, since this technology utilizes surface chemistry to attach the capture modality to the substrate surface, the SATCR capture surface could be adapted aptamers . Indeed, many researchers have already discussed the importance of protein coatings, or “coronas.” Ultimately, this chemistry and device can be commercialized and distributed as a customizable, rapid, low‐cost, research, and diagnostic tool.…”

Section: Discussionmentioning

confidence: 99%

Cell isolation via spiral microfluidics and the secondary anchor targeted cell release system

et al. 2019

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Precision medicine requires high throughput cell isolation and measurement that maintains physiology. Unfortunately, many techniques are slow or alter cell biomarkers cells.This necessitates new approaches, which we achieve by integrating affinity-based cell isolation with spiral microfluidics. We characterize the device via computational simulations, predicting wall shear stress within an order of magnitude of arterial wall shear stress (~0.2 Pa). We identify that poly-L-lysine supplementation preserves cell geometry and improves cell release. We demonstrate preservation of angiogenic biomarker concentrations, measuring 1,000-2,000 vascular endothelial growth factor receptor-1 per human umbilical vein endothelial cell, which is in line with the previously reported measurements. We attain 76.7 ± 9.0% release of captured cells by integrating thermophoresis and optimizing buffer residence time. Ultimately, we find that combining affinity-based cell isolation (secondary anchor targeted cell release) with spiral microfluidics offers a fast, biomarker preserving approach needed to individualize medicine.

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Section: Discussionmentioning

confidence: 99%

Cell isolation via spiral microfluidics and the secondary anchor targeted cell release system

et al. 2019

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“…However, the shortcomings of chemotherapeutic drugs are inevitable owing to serious toxic side effects, such as their poor water solubility, nonspecific distribution and systemic toxicity greatly reduce delivery efficiency, which limits their use in clinic. Up to now, targeted chemotherapy is vital to avoid its side effects and enhance its therapeutic efficiency 135 . Aptamers conjugated with chemotherapeutic drugs as a kind of targeted therapeutic mean would increase drugs delivery effect and reduce cytotoxicity of drugs in normal tissue.…”

Section: Aptamer-chemotherapy Drugs Conjugatesmentioning

confidence: 99%

Application and development of aptamer in cancer: from clinical diagnosis to cancer therapy

et al. 2020

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Traditional anticancer therapies can cause serious side effects in clinical treatment due to their nonspecific of tumor cells. Aptamers, also termed as 'chemical antibodies', are short DNA or RNA oligonucleotides selected from the synthetic large random single-strand oligonucleotide library by systematic evolution of ligands by exponential enrichment (SELEX) to bind to lots of different targets, such as proteins or nucleic acid structures. Aptamers have good affinities and high specificity with target molecules, thus may be able to act as drugs themselves to directly inhibit the proliferation of tumor cells, or own great potentialities in the targeted drug delivery systems which can be used in tumor diagnosis and target specific tumor cells, thereby minimizing the toxicity to normal cells. Here we review the unique properties of aptamer represents a great opportunity when applied to the rapidly developing fields of biotechnology and discuss the recent developments in the use of aptamers as powerful tools for analytic, diagnostic and therapeutic applications for cancer.

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“…Breast cancer is one of the worldwide leading cancers among women and the leading cause in cancer death in most countries . Classification of breast cancers depends on positivity or negativity expression of estrogen receptors (ER), progesterone receptors (PR), and human epidermal growth factor 2 receptor (HER2) . Early diagnosis and appropriate adjusted treatment of breast cancer can prolong disease free‐survival.…”

Section: Figurementioning

confidence: 99%

Rapid Detection of AIB1 in Breast Cancer Cells Based on Aptamer‐Functionalized Nanomotors

et al. 2019

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Herein, we report ultrasound‐propelled graphene‐oxide coated gold nanowire motors, functionalized with fluorescein‐labeled DNA aptamers (FAM‐AIB1‐apt), for qualitative detection of overexpressed AIB1 oncoproteins in MCF‐7 breast cancer cells. The movement of nanomotors under the ultrasound field facilitated intracellular uptake and resulted in a faster aptamer binding with the target protein and thus faster fluorescence recovery. The propulsion behavior of the aptamer functionalized nanomotors greatly enhanced the fluorescence intensity compared to static conditions. The new aptamer@nanomotor‐based strategy offers considerable potential for further development of sensing methodologies towards diagnosis of breast cancer.

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Aptamer selection and applications for breast cancer diagnostics and therapy

Cited by 111 publications

References 140 publications

Cell isolation via spiral microfluidics and the secondary anchor targeted cell release system

Cell isolation via spiral microfluidics and the secondary anchor targeted cell release system

Application and development of aptamer in cancer: from clinical diagnosis to cancer therapy

Rapid Detection of AIB1 in Breast Cancer Cells Based on Aptamer‐Functionalized Nanomotors

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