Aptamer photoregulation in vivo

Li, Lele; Tong, Rong; Chu, Hunghao; Wang, Weiping; Langer, Robert; Kohane, Daniel S.

doi:10.1073/pnas.1420105111

Cited by 61 publications

(59 citation statements)

References 55 publications

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“…It was shown that the adsorption of aromatic molecules on silver is often accompanied by the metal-adsorbate charge transfer that is strongly affected by the surface potential. [77][78][79] The above results for neocuproine suggest that the freer rotation of the methyl groups at more negative electrode potential takes place due to the charge transfer process. Apparently when the electrode potential is shifted to more negative values, the charge transfer is activated, and in the vicinity of the zero charge potential (ca.…”

Section: Resultsmentioning

confidence: 99%

SERS investigation of neocuproine adsorption on silver: Influence of electrode potential on methyl groups

Solovyeva

Rakhimbekova

Lanchuk

et al. 2017

J Raman Spectroscopy

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Study of molecules adsorption on charged surfaces is important for biologically relevant substances where the potential at the interface such as living cell membrane is a significant parameter in the processes of their transportation or transmembrane penetration. In this work, a hybrid optical/electrochemical surface‐enhanced Raman scattering (SERS) technique was applied to get new insight into the adsorption state and conformational equilibrium of neocuproine, which serves as a nucleic acid biosensor in clinical diagnostics and has biological activity towards several types of carcinoma. The density functional theory calculations performed for several rotational conformations and their anion radicals were used to determine the geometrical and energetic characteristics, to evaluate the rotational barrier, to obtain the vibrational assignment, and to consider the metal‐adsorbate charge transfer. The dependence of SERS spectra on surface potential is ascribed to a change of the rotational dynamics of methyl groups from hindered to almost free at potentials ≤−200 mV. It is demonstrated for the first time that SERS spectroscopy is capable to recognize the surface species, which differ in the methyl group internal rotation.

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Section: Resultsmentioning

confidence: 99%

SERS investigation of neocuproine adsorption on silver: Influence of electrode potential on methyl groups

Solovyeva

Rakhimbekova

Lanchuk

et al. 2017

J Raman Spectroscopy

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“…Furthermore, the answer that whether endocytosis increase in ovarian cancer compared to normal should be revealed? If the answer is yes, chemotherapy or radiotherapy agents, famous for their side effects, can be coupled to aptamers [34].…”

Section: Discussionmentioning

confidence: 99%

Characterization of a DNA Aptamer for Ovarian Cancer Clinical Tissue Recognition and in Vivo Imaging

Wang

Zhang

et al. 2018

Cell Physiol Biochem

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Backgrounds/Aims: Ovarian cancer is the most lethal gynaecologic malignancy and is difficult to detect early. The inefficient early diagnosis of ovarian cancer is the main contributor to its high mortality rate. Aptamers, as chemical antibodies, are single-stranded DNA or RNA oligonucleotides that target cells or molecules with high affinity. Methods: Binding ability of R13 was measured by flow cytometry analysis. Stability of R13 was tested in blood serum of an ovarian cancer patient. Internalization of R13 was verified by confocal microscope imaging. 80 cases ovarian cancer tissues, 10 cases normal ovary tissues in a microarray and 6 fallopian tube tissues were prepared for this study. R13’s target ability was further confirmed in vivo tumor models in NOD/SCID mice. Results: In this study, we found aptamer R13 bound to ovarian cancer cells with dissociation constants in the nanomolar range. Moreover, these results were further confirmed by tissue imaging. Next we demonstrated that the targets of R13 are membrane proteins and that its internalization occurs in a caveolae-mediated and clathrin-mediated manner. The target function of R13 was determined by imaging A2780 tumours in mouse models. Conclusion: These findings suggest that R13 is a promising novel tool to diagnose and deliver drugs to treat ovarian cancer.

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“…This experiment thus permits the examination of the overlap of 3HP SDA-generated green fluorescence and DT1-generated red fluorescence inside cells.DT1 has two sequence elements, one for binding AS1411 (to achieve cancer cell targeting) and the other for binding HPa (to initiate 3HP SDA). AS1411 is aD NA aptamer with high affinity for nucleolin known to express on the surface of various cancer cells, [18] and has been successfully used in previous studies for both cancer cell targeting [19] and selective internalization to the surface of nuclei. [20] In this experiment, AS1411 was modified to contain asequence tag that binds DT1.…”

Section: Angewandte Chemiementioning

confidence: 99%

Target‐Induced Catalytic Assembly of Y‐Shaped DNA and Its Application for In Situ Imaging of MicroRNAs

et al. 2018

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Aptamer photoregulation in vivo

Cited by 61 publications

References 55 publications

SERS investigation of neocuproine adsorption on silver: Influence of electrode potential on methyl groups

SERS investigation of neocuproine adsorption on silver: Influence of electrode potential on methyl groups

Characterization of a DNA Aptamer for Ovarian Cancer Clinical Tissue Recognition and in Vivo Imaging

Target‐Induced Catalytic Assembly of Y‐Shaped DNA and Its Application for In Situ Imaging of MicroRNAs

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