Aptamer/magnetic nanoparticles decorated with fluorescent gold nanoclusters for selective detection and collection of human promyelocytic leukemia (HL-60) cells from a mixture

Abstract

In the present work, a fluorescent gold nanoclusters (GNCs)/superparamagnetic (Fe 3 O 4 /GNCs) nanoprobe was prepared via a facile approach for the selective detection and imaging of human leukemica cancer cells (HL-60). (γ-Mercaptopropyl)trimethoxysilane (MPS) was used as a stabilizer to prepare functionalized GNCs. The prepared GNCs@MPS was then self-assembly decorated on the surface of Fe 3 O 4 @SiO 2 nanoparticles followed by poly(ethylene glycol) dimethacrylate (PGD) addition at room temperature to form Fe 3 O 4 /GNCs nanoprobe. Surface functionalization of the Fe 3 O 4 /GNCs with the thiol-modified KH1C12 aptamer was done through thiol-en click reaction between PGD and the thiol group of the aptamer. An extensive characterization of the Fe 3 O 4 /GNCs revealed strong red fluorescence (λ em =627 nm), T 2 -based contrast agent for MRI and excellent colloidal and photo stability in buffer medium. So, the aptamer-functionalized Fe 3 O 4 /GNCs nanoprobe (Fe 3 O 4 /GNCs/Aptamer) is capable to uptake and dual-image HL-60 cancer cells from a mixture. Furthermore, the MRI signal intensity of the pictures decreased linearly with an increase in the concentrations of the nanoprobe. It is also enable to detect cancer cells from a range of concentrations 10 up to 200 cells μL −1 . The fluorescent/magnetic characteristics of the nanoprobe are of great significance for MRI-based and fluorescence imaging and collection of HL-60 cancer cells which implies potential help for the development of early diagnosis of highly malignant human leukemia.

<p>Aptamer-Conjugated Gold Nanoparticles Targeting Epidermal Growth Factor Receptor Variant III for the Treatment of Glioblastoma</p>

“…Gold nanoparticles with aptamer have been widely applied in biosensors and imaging but not in brain-targeting delivery carriers. [32][33][34] However, no study has been performed to evaluate the effects of aptamers conjugated with gold nanoparticles to treat glioma. In this report, we tentatively propose that the U2-AuNP complex could effectively cross the blood-brain barrier and accumulate around GBM in vivo.…”

Theranostic Potentials of Gold Nanomaterials in Hematological Malignancies

“…(1) detection of leukemia or lymphoma cells based on unique antigen-receptor genes of Tand B-cells, (2) detection of genetic mutation(s), (3) identification of chromosomal deletions, translocations, or duplications, and (4) proteomic changes [71][72][73][74]. This section will report the accuracy, specificity, and reliability of aptamer, biocompatible polymer, antibody, nucleic acid, or ligand-tuned GNMs in the diagnosis of HMs.…”

“…Haghighi et al, (2020) [71] reported that gold nanocluster (GNC) modified with Fe3O4 and KH1C12 aptamer selectively diagnosed highly malignant HL-60 human-leukemia cells from a mixture of different cells. This nanoprobe could be used in MRI imaging (T 2 -based) or fluorescent-microscopy-based diagnosis of leukemia cells (as low as 10 cells µL −1 ).…”

Nanotechnology in Tumor Biomarker Detection: The Potential of Liganded Nanoclusters as Nonlinear Optical Contrast Agents for Molecular Diagnostics of Cancer

“…Finally, nanomaterials can be detected in vivo, e.g., in xenografted mice using MRI, nuclear imaging methods (SPECT, PET/CT, autoradiography), NIR fluorescence imaging systems, or photoacoustic imaging. For MRI, it is required for the nanomaterials to include or to be coated with a heavy metal such as iron oxide or gadolinium [72,73]. On the other hand, nuclear imaging methods require the labeling of the nanomaterials with radioisotopes [74].…”