Aptamer-functionalized nanoparticles for surface immobilization-free electrochemical detection of cortisol in a microfluidic device

Sanghavi, Bankim J.; Moore, J.; Chávez, Jorge L.; Hagen, Joshua A.; Kelley‐Loughnane, Nancy; Chou, Chia-Fu; Swami, Nathan S.

doi:10.1016/j.bios.2015.11.044

Cited by 161 publications

(80 citation statements)

References 51 publications

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“…Alternate approaches include the measurement of bacterial metabolites, biomolecular nucleic acid-based tests 13,14 , and antibody-based immunoassays 15,16 , whereas some of the emerging methods include those based on impedance and Raman techniques. While sample collection at scales of ≥0.1 L remains a major challenge, all of these detection modalities could benefit from microfluidic sample manipulation 17 for enhancing capture of the target microbial cell at the sensing surface under continuous flow 18,19 and/or selective sample enrichment conditions 20 . Specifically, through volume confinement at the scale of single cells 21 to locally enrich secreted analytes in vicinity of the sensor, the laminar flows within microfluidic systems can locally and rapidly modulate pressure differentials to control chemical and physical environments 22 , create conditions for constant nutrient replenishment 23 and enable rapid switching of media 24 .…”

Section: Introductionmentioning

confidence: 99%

Review: Microbial analysis in dielectrophoretic microfluidic systems

Fernandez

Rohani

Farmehini

et al. 2017

Analytica Chimica Acta

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122

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Infections caused by various known and emerging pathogenic microorganisms, including antibiotic-resistant strains, are a major threat to global health and well-being. This highlights the urgent need for detection systems for microbial identification, quantification and characterization towards assessing infections, prescribing therapies and understanding the dynamic cellular modifications. Current state-of-the-art microbial detection systems exhibit a trade-off between sensitivity and assay time, which could be alleviated by selective and label-free microbial capture onto the sensor surface from dilute samples. AC electrokinetic methods, such as dielectrophoresis, enable frequency-selective capture of viable microbial cells and spores due to polarization based on their distinguishing size, shape and sub-cellular compositional characteristics, for downstream coupling to various detection modalities. Following elucidation of the polarization mechanisms that distinguish bacterial cells from each other, as well as from mammalian cells, this review compares the microfluidic platforms for dielectrophoretic manipulation of microbials and their coupling to various detection modalities, including immuno-capture, impedance measurement, Raman spectroscopy and nucleic acid amplification methods, as well as for phenotypic assessment of microbial viability and antibiotic susceptibility. Based on the urgent need within point-of-care diagnostics towards reducing assay times and enhancing capture of the target organism, as well as the emerging interest in isolating intact microbials based on their phenotype and subcellular features, we envision widespread adoption of these label-free and selective electrokinetic techniques.

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Section: Introductionmentioning

confidence: 99%

Review: Microbial analysis in dielectrophoretic microfluidic systems

Fernandez

Rohani

Farmehini

et al. 2017

Analytica Chimica Acta

Self Cite

122

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“…An ideal microfluidic readout system should be fast, portable, sensitive and quantitative, while allowing the detection of a wide range of targets [83,97]. AuNPs have been implemented in microfluidic systems for biomolecular detection of nucleic acids, proteins, and small molecules [98,99] improving the sensitivity and specificity of the assays, and expanding their range of detection.…”

Section: General Overview Of Applicationsmentioning

confidence: 99%

“…Other platforms for the detection of metabolites of clinical relevance have been reported, such as cortisol [98], glucose [99] and dopamine [103]. Additionally, the use of these platforms for DNA molecular diagnostics of human pathologies has been proposed with the potential for discrimination of point mutations [104,105].…”

Section: General Overview Of Applicationsmentioning

confidence: 99%

Gold Nanoparticles for Diagnostics: Advances towards Points of Care

et al. 2016

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The remarkable physicochemical properties of gold nanoparticles (AuNPs) have prompted developments in the exploration of biomolecular interactions with AuNP-containing systems, in particular for biomedical applications in diagnostics. These systems show great promise in improving sensitivity, ease of operation and portability. Despite this endeavor, most platforms have yet to reach maturity and make their way into clinics or points of care (POC). Here, we present an overview of emerging and available molecular diagnostics using AuNPs for biomedical sensing that are currently being translated to the clinical setting.

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“…In the assay developed by [51], target recognition by the aptamer and signal production are independent and thus, could be optimized separately for better specificity. Figure 4 illustrates the mechanism of surface immobilization-free electrochemical detection of cortisol.…”

Section: Cortisol Aptasensor Towards Point-of-care Diagnosticsmentioning

confidence: 99%

Current and Potential Developments of Cortisol Aptasensing towards Point-of-Care Diagnostics (POTC)

Abidin

Ruslinda

Arshad

et al. 2017

Sensors

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Anxiety is a psychological problem that often emerges during the normal course of human life. The detection of anxiety often involves a physical exam and a self-reporting questionnaire. However, these approaches have limitations, as the data might lack reliability and consistency upon application to the same population over time. Furthermore, there might be varying understanding and interpretations of the particular question by the participant, which necessitating the approach of using biomarker-based measurement for stress diagnosis. The most prominent biomarker related to stress, hormone cortisol, plays a key role in the fight-or-flight situation, alters the immune response, and suppresses the digestive and the reproductive systems. We have taken the endeavour to review the available aptamer-based biosensor (aptasensor) for cortisol detection. The potential point-of-care diagnostic strategies that could be harnessed for the aptasensing of cortisol were also envisaged.

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Aptamer-functionalized nanoparticles for surface immobilization-free electrochemical detection of cortisol in a microfluidic device

Cited by 161 publications

References 51 publications

Review: Microbial analysis in dielectrophoretic microfluidic systems

Review: Microbial analysis in dielectrophoretic microfluidic systems

Gold Nanoparticles for Diagnostics: Advances towards Points of Care

Current and Potential Developments of Cortisol Aptasensing towards Point-of-Care Diagnostics (POTC)

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