Aptamer functionalized magnetic nanoparticles for effective extraction of ultratrace amounts of aflatoxin M1 prior its determination by HPLC

Khodadadi, Mohammad; Malekpour, Akbar; Mehrgardi, Masoud A.

doi:10.1016/j.chroma.2018.06.022

Cited by 52 publications

(19 citation statements)

References 43 publications

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“…They prepared a magnetic nanoparticle‐based sorbent and characterized by employing various methods then used in detection of AFM1 in milk samples. The detection limit of method was reported as 0.2 ng/L . In another study, Amoll‐Diva et al used hydrophobic oleic acid modified Fe 3 O 4 to remove the analyte from dispersing liquid–liquid microextraction step with the VA‐D‐SPE‐supported method.…”

Section: Resultsmentioning

confidence: 99%

Spectrofluorimetric Determination of Aflatoxin B₁ in Winter Herbal Teas via Magnetic Solid Phase Extraction Method by using Metal–Organic Framework (MOF) Hybrid Structures Anchored with Magnetic Nanoparticles

Durmuș

Kurt

Gazioğlu

et al. 2020

Applied Organom Chemis

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MIL53(Al)‐SiO2@Fe3O4 composite was prepared by co‐precipitation route with a typical Stöber synthetic process and ultrasonic‐agitation, then subsequently utilized as a multi‐component novel sorbent in solid‐phase microextraction (SPME) of aflatoxin B1 in winter herbal teas. Microstructural properties of MIL53(Al)‐SiO2@Fe3O4 composite was characterized by using Fourier transform infrared (FTIR) spectroscopy, powder X‐ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM) methods, and Brunauer–Emmett–Teller (BET) surface area measurement. The MIL53(Al)‐SiO2@Fe3O4 composite was found to be a very effective sorbent in spectrofluorimetric determination of aflatoxin B1 (AFB1) in winter herbal teas via magnetic solid‐phase extraction (SPE) route. The proposed method showed a wide linear range from 0.5 to 150 ng/ml, low limit of detection (LOD = 0.5 ng/ml), and an acceptable recovery values (70.7–96.5%) in real samples analysis. This study shows that the suggested method possesses an important potential to use for detecting AFB1 in quality control laboratories.

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Section: Resultsmentioning

confidence: 99%

Spectrofluorimetric Determination of Aflatoxin B₁ in Winter Herbal Teas via Magnetic Solid Phase Extraction Method by using Metal–Organic Framework (MOF) Hybrid Structures Anchored with Magnetic Nanoparticles

Durmuș

Kurt

Gazioğlu

et al. 2020

Applied Organom Chemis

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“…On the other hand, another common coating material of MNPs is silica, which in turn, due to the presence of silanol groups in its surface, it can be functionalized with different organosilanes and/or affinity ligands which enable the selective extraction of analytes. In this context, aptamer-functionalized silica MNPs have been proposed as sorbents for the extraction of aflatoxin M1 from dairy products [63]. More recently, magnetic mesoporous silica nanoparticles have been bifunctionalized with octadecyl (C18) and amino (NH 2 ) groups in order to obtain a magnetic mixed-mode sorbent with reversed-phase/weak anion exchange interactions [64].…”

Section: Micro-magnetic Solid-phase Extraction (µ-Mspe)mentioning

confidence: 99%

New Advanced Materials and Sorbent-Based Microextraction Techniques as Strategies in Sample Preparation to Improve the Determination of Natural Toxins in Food Samples

2020

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Natural toxins are chemical substances that are not toxic to the organisms that produce them, but which can be a potential risk to human health when ingested through food. Thus, it is of high interest to develop advanced analytical methodologies to control the occurrence of these compounds in food products. However, the analysis of food samples is a challenging task because of the high complexity of these matrices, which hinders the extraction and detection of the analytes. Therefore, sample preparation is a crucial step in food analysis to achieve adequate isolation and/or preconcentration of analytes and provide suitable clean-up of matrix interferences prior to instrumental analysis. Current trends in sample preparation involve moving towards “greener” approaches by scaling down analytical operations, miniaturizing the instruments and integrating new advanced materials as sorbents. The combination of these new materials with sorbent-based microextraction technologies enables the development of high-throughput sample preparation methods, which improve conventional extraction and clean-up procedures. This review gives an overview of the most relevant analytical strategies employed for sorbent-based microextraction of natural toxins of exogenous origin from food, as well as the improvements achieved in food sample preparation by the integration of new advanced materials as sorbents in these microextraction techniques, giving some relevant examples from the last ten years. Challenges and expected future trends are also discussed.

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“…With the advantage of accuracy, liquid chromatography has been used as a reference method for examining various small compounds. However, detecting low levels of AFM1 in milk with chromatographic methods generally needs a concentration and purification procedure through immunoaffinity columns, which is costly and time-consuming [ 18 ]. ELISA has also been applied in determining the levels of AFM1 and CAP residues for its speed, low-cost, and high-throughput ability.…”

Section: Introductionmentioning

confidence: 99%

“…The immunochromatographic strip is a simple, rapid, and multi-target technology suitable for on-site detection of natural toxins and drug residues by untrained personnel. Several studies demonstrate the use of immunostrip assays to determine the level of a single contaminant, either AFM1 or CAP, in milk samples [ 16 , 17 , 18 , 19 , 20 ]. In the present study, a two-analyte immunostrip assay was established for the first time to monitor AFM1 and CAP contamination at the same time with a concept of antigen competition.…”

Section: Introductionmentioning

confidence: 99%

A Sensitive Two-Analyte Immunochromatographic Strip for Simultaneously Detecting Aflatoxin M1 and Chloramphenicol in Milk

Liu

et al. 2020

Toxins

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A two-analyte immunochromatographic strip (immunostrip) was developed for the simultaneous detection of aflatoxin M1 (AFM1) and chloramphenicol (CAP) in milk. Protein conjugates (AFM1-ovalbumin (OVA) and CAP-OVA) and goat anti-rabbit IgG were respectively drawn on nitrocellulose membrane as two test lines (T1 and T2) and a control line (C). The immunostrip was dipped into a well that contained a 200 μL milk sample, 5 μL AFM1 antibody-gold conjugates, and 8 μL CAP antibody-gold conjugates; the whole assay was completed in 15 min and the results could be interpreted visually or using a reader. This immunostrip has cut-off levels of 0.1 ng/mL and 0.5 ng/mL for AFM1 and CAP, respectively. Analysis of CAP and AFM1 in milk samples revealed that data from the immunostrip test agreed closely with those obtained from ELISA. The two-analyte immunostrip is a rapid way for on-site simultaneous detection of AFM1 and CAP in milk.

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Aptamer functionalized magnetic nanoparticles for effective extraction of ultratrace amounts of aflatoxin M1 prior its determination by HPLC

Cited by 52 publications

References 43 publications

Spectrofluorimetric Determination of Aflatoxin B₁ in Winter Herbal Teas via Magnetic Solid Phase Extraction Method by using Metal–Organic Framework (MOF) Hybrid Structures Anchored with Magnetic Nanoparticles

Spectrofluorimetric Determination of Aflatoxin B₁ in Winter Herbal Teas via Magnetic Solid Phase Extraction Method by using Metal–Organic Framework (MOF) Hybrid Structures Anchored with Magnetic Nanoparticles

New Advanced Materials and Sorbent-Based Microextraction Techniques as Strategies in Sample Preparation to Improve the Determination of Natural Toxins in Food Samples

A Sensitive Two-Analyte Immunochromatographic Strip for Simultaneously Detecting Aflatoxin M1 and Chloramphenicol in Milk

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Aptamer functionalized magnetic nanoparticles for effective extraction of ultratrace amounts of aflatoxin M1 prior its determination by HPLC

Cited by 52 publications

References 43 publications

Spectrofluorimetric Determination of Aflatoxin B1 in Winter Herbal Teas via Magnetic Solid Phase Extraction Method by using Metal–Organic Framework (MOF) Hybrid Structures Anchored with Magnetic Nanoparticles

Spectrofluorimetric Determination of Aflatoxin B1 in Winter Herbal Teas via Magnetic Solid Phase Extraction Method by using Metal–Organic Framework (MOF) Hybrid Structures Anchored with Magnetic Nanoparticles

New Advanced Materials and Sorbent-Based Microextraction Techniques as Strategies in Sample Preparation to Improve the Determination of Natural Toxins in Food Samples

A Sensitive Two-Analyte Immunochromatographic Strip for Simultaneously Detecting Aflatoxin M1 and Chloramphenicol in Milk

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Spectrofluorimetric Determination of Aflatoxin B₁ in Winter Herbal Teas via Magnetic Solid Phase Extraction Method by using Metal–Organic Framework (MOF) Hybrid Structures Anchored with Magnetic Nanoparticles

Spectrofluorimetric Determination of Aflatoxin B₁ in Winter Herbal Teas via Magnetic Solid Phase Extraction Method by using Metal–Organic Framework (MOF) Hybrid Structures Anchored with Magnetic Nanoparticles