Aptamer-based highly sensitive electrochemiluminescent detection of thrombin via nanoparticle layer-by-layer assembled amplification labels

Chen, Ying; Jiang, Bingying; Xiang, Yun; Chai, Yaqin; Yuan, Ruo

doi:10.1039/c1cc12249e

Cited by 46 publications

(22 citation statements)

References 38 publications

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“…Water soluble MPA-capped CdTe QDs (MPA-CdTe) were prepared according to our previously reported protocol [37]. The mean size of the QDs is estimated from the corresponding UV-Vis adsorption peak to be 3.1 nm and thus the concentration of the QDs solution is calculated to be 140 µM on the basis of the Peng's empirical equation [36].…”

Section: Preparation Of the Dox-qd Nanoconjugatesmentioning

confidence: 99%

Intercalation of quantum dots as the new signal acquisition and amplification platform for sensitive electrochemiluminescent detection of microRNA

Chen

Xiang

Yuan

2015

Analytica Chimica Acta

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Section: Preparation Of the Dox-qd Nanoconjugatesmentioning

confidence: 99%

Intercalation of quantum dots as the new signal acquisition and amplification platform for sensitive electrochemiluminescent detection of microRNA

Chen

Xiang

Yuan

2015

Analytica Chimica Acta

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“…With the increasing requirements for the sensitivity of biosensing system, various signal amplification approaches have been developed in recent years, such as nanoparticle-based electrochemical methods (Chen et al 2011;Zhou, Duan, and Xing 2012), rolling cycle amplification (W. Cheng et al 2010;Zhao et al 2008), and amplification strategies based on polymerase (Shi et al 2011), nicking endonuclease (Mi et al 2012; X. L. Zhu et al 2011) and Exonuclease III (X. Niu et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Determination of Adenosine Triphosphate by a Target Inhibited Catalytic Cycle Based on a Strand Displacement Reaction

Cheng¹,

Zheng²,

Wang

et al. 2014

Analytical Letters

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A strand displacement reaction-based system was developed for the determination of adenosine triphosphate (ATP). It involved an entropy-driven catalytic cycle that directly employed the ATP aptamer as the catalyst. Introduction of ATP into the system induced the catalyst to form the G-quadruplex conformation and inhibited its catalytic activity. All intermediates in the catalytic cycle processes were identified by polyacrylamide gel electrophoresis analysis. When the oligonucleotides were labeled with a carboxyfluorescein fluorophore and a 4-([4-(dimethylamino)phenyl]azo)benzoic acid quencher, this strand displacement reaction-based catalytic system exhibited a ''switch-on'' response for ATP. Conditions for detecting ATP, such as the toehold length, concentrations of the catalyst and magnesium ion, and incubation temperature, were optimized to obtain a detection limit of 50 nM and a linear response up to 1400 nM of ATP. This target inhibited catalytic cycle provides an enzyme-free biosensing strategy and has potential application in aptamer-based biosensing.

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“…Up to now, various aptamer‐based detection systems for thrombin have been developed with different signal amplification platforms 23–28. Many biomaterials, such as ferrocene 29, 30, graphene 31–34, metal nanoparticles 35–38 and enzymes 39 are used as labels for signal amplification in such aptamer‐based detection systems for their outstanding physical and chemical properties.…”

Section: Introductionmentioning

confidence: 99%

G‐Quadruplex‐Linked Supersandwich DNA Structure for Electrochemical Amplified Detection of Thrombin

Wang

Zhu

et al. 2013

Electroanalysis

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In this paper, a novel strategy of electrochemical amplified detection of thrombin based on G‐quadruplex‐linked supersandwich structure was described. In the presence of K+ and hemin, the original hairpin DNA sequence activated an autonomous cross‐opening process to build up hemin/G‐quadruplex structure and can hybridize to form supersandwich structure containing multiple signal labels. With the addition of thrombin, it conjugated with its aptamer, leading to a remarkably descended signal. The supersandwich‐amplified electrochemical sensor system was highly sensitive in the concentration range from 10−6 to 10−10 M with a detection limit of 10 pM and also demonstrated excellent selectivity. The amplifying supersandwich structure with multiple labels can be implemented as a versatile sensing platform for analyzing other DNA in the presence of the appropriate probe.

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Aptamer-based highly sensitive electrochemiluminescent detection of thrombin via nanoparticle layer-by-layer assembled amplification labels

Cited by 46 publications

References 38 publications

Intercalation of quantum dots as the new signal acquisition and amplification platform for sensitive electrochemiluminescent detection of microRNA

Intercalation of quantum dots as the new signal acquisition and amplification platform for sensitive electrochemiluminescent detection of microRNA

Determination of Adenosine Triphosphate by a Target Inhibited Catalytic Cycle Based on a Strand Displacement Reaction

G‐Quadruplex‐Linked Supersandwich DNA Structure for Electrochemical Amplified Detection of Thrombin

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