Aptamer-based fluorescent and visual biosensor for multiplexed monitoring of cancer cells in microfluidic paper-based analytical devices

Li, Liang; Su, Min; Li, Li; Lan, Feifei; Yang, Gangyi; Ge, Shenguang; Yu, Jinghua; Song, Xianrang

doi:10.1016/j.snb.2016.01.137

Cited by 134 publications

(79 citation statements)

References 38 publications

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“…Liang et al have developed another fluorescent and multiplexed aptasensor for monitoring cancer cells in µPADs that can be observed by a naked eye [79]. The device utilizes quantum dotscoated mesoporous silica nanoparticles-labeled aptamers that are attached to the surface of GO.…”

Section: Fluorescence Detectionmentioning

confidence: 99%

Lab-on-a-Chip Systems for Aptamer-Based Biosensing

Khan

Song

2020

Micromachines

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Aptamers are oligonucleotides or peptides that are selected from a pool of random sequences that exhibit high affinity toward a specific biomolecular species of interest. Therefore, they are ideal for use as recognition elements and ligands for binding to the target. In recent years, aptamers have gained a great deal of attention in the field of biosensing as the next-generation target receptors that could potentially replace the functions of antibodies. Consequently, it is increasingly becoming popular to integrate aptamers into a variety of sensing platforms to enhance specificity and selectivity in analyte detection. Simultaneously, as the fields of lab-on-a-chip (LOC) technology, point-of-care (POC) diagnostics, and personal medicine become topics of great interest, integration of such aptamer-based sensors with LOC devices are showing promising results as evidenced by the recent growth of literature in this area. The focus of this review article is to highlight the recent progress in aptamer-based biosensor development with emphasis on the integration between aptamers and the various forms of LOC devices including microfluidic chips and paper-based microfluidics. As aptamers are extremely versatile in terms of their utilization in different detection principles, a broad range of techniques are covered including electrochemical, optical, colorimetric, and gravimetric sensing as well as surface acoustics waves and transistor-based detection.

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Section: Fluorescence Detectionmentioning

confidence: 99%

Lab-on-a-Chip Systems for Aptamer-Based Biosensing

Khan

Song

2020

Micromachines

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“…While with the strip reader, the detection limit of this strip biosensor could reach down to 800 Ramos cells ( Figure ) . Fluorescence was also adopted for detecting cancer cells in a wax‐printed paper microfluidic system, in which aptamers were used to capture cancer cell with specific affinity. Graphene oxide (GO)‐based aptameric nanosensor integrated the exceptional quenching capability of GO with the high specificity and affinity of aptamer.…”

Section: Cell Capture/phenotyping On Paper Microfluidic Devicesmentioning

confidence: 99%

Paper Microfluidics for Cell Analysis

Yan

Miao

et al. 2018

Adv Healthcare Materials

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Paper microfluidics has attracted much attention since its first introduction around one decade ago due to the merits such as low cost, ease of fabrication and operation, portability, and facile integration with other devices. The dominant application for paper microfluidics still lies in point‐of‐care testing (POCT), which holds great promise to provide diagnostic tools to meet the ASSURED criteria. With micro/nanostructures inside, paper substrates provide a natural 3D scaffold to mimic native cellular microenvironments and create excellent biointerfaces for cell analysis applications, such as long‐term 3D cell culture, cell capture/phenotyping, and cell‐related biochemical analysis (small molecules, protein DNA, etc.). This review summarizes cell‐related applications based on various engineered paper microdevices and provides some perspectives for paper microfluidics‐based cell analysis.

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“…130 It was coupled with quantum dots, and the fluorescence quenching transfer was visible with the naked eye. 130 It was coupled with quantum dots, and the fluorescence quenching transfer was visible with the naked eye.…”

Section: Aptamer-based Cancer Detectionmentioning

confidence: 99%

“…This device could detect PSA in the 10-1,000 ng/mL range, indicating that it has the potential for use in point-of-care diagnostics. 130 systems with the aim of proving a rapid, sensitive option for cancer diagnostics. 132 These parallel assays were coupled with a secondary antibody or a lectin and quantified by chemiluminescence.…”

Section: Aptamer-based Cancer Detectionmentioning

confidence: 99%

Microfluidics in the selection of affinity reagents for the detection of cancer: paving a way towards future diagnostics

Hung

Wang

et al. 2016

Lab Chip

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Microfluidic technologies have miniaturized a variety of biomedical applications, and these chip-based systems have several significant advantages over their large-scale counterparts. Recently, this technology has been used for automating labor-intensive and time-consuming screening processes, whereby affinity reagents, including aptamers, peptides, antibodies, polysaccharides, glycoproteins, and a variety of small molecules, are used to probe for molecular biomarkers. When compared to conventional methods, the microfluidic approaches are faster, more compact, require considerably smaller quantities of samples and reagents, and can be automated. Furthermore, they allow for more precise control of reaction conditions (e.g., pH, temperature, and shearing forces) such that more efficient screening can be performed. A variety of affinity reagents for targeting cancer cells or cancer biomarkers are now available and will likely replace conventional antibodies. In this review article, the selection of affinity reagents for cancer cells or cancer biomarkers on microfluidic platforms is reviewed with the aim of highlighting the utility of such approaches in cancer diagnostics.

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Aptamer-based fluorescent and visual biosensor for multiplexed monitoring of cancer cells in microfluidic paper-based analytical devices

Cited by 134 publications

References 38 publications

Lab-on-a-Chip Systems for Aptamer-Based Biosensing

Lab-on-a-Chip Systems for Aptamer-Based Biosensing

Paper Microfluidics for Cell Analysis

Microfluidics in the selection of affinity reagents for the detection of cancer: paving a way towards future diagnostics

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