Aptamer-assisted novel technologies for detecting bacterial pathogens

Current and Emerging Technologies for Rapid Detection of Pathogens

“…The frequent outbreak of foodborne diseases and the economic and social implications indicate that analytical methodologies that can rapidly detect and identify pathogens are urgently needed. As such, many researchers devote themselves to developing more advanced detection methods that can identify pathogens accurately and rapidly in a timely manner in the food industry [13][14][15][16][17][18][19][20].…”

Novel diagnostics for point-of-care bacterial detection and identification

“…32 2.2 Aptamers 15,33 Aptamers are short, single-stranded DNA or RNA oligonucleotide biosensors that can interact with a wide variety of analyte targets with high affinity and specicity. 15,34 Upon binding to their target analyte, aptamers fold into specic threedimensional structures with many surface interactions for strong bonding, 34 typically with a dissociation constant in the nano-or pico-molar level. 35,36 Aptamers are chemically synthesized in vitro by a process known as Systematic Evolution of Ligands by Exponential enrichment (SELEX), which involves three key steps: (1) the incubation of an oligonucleotide sequence library with the target analyte to assess which structures bind; (2) the elution of unbound oligonucleotides, separating them from those bound to the analyte; and (3) amplication of the remaining oligonucleotide sequences by PCR.…”

“…These three steps can be repeated up to 20 times until a small collection of sequences with high affinity for the target are identied. 34,35 When used as a biosensor, aptamers offer many advantages over antibodies; in contrast to antibodies, aptamers have low toxicity, are stable over a wide temperature and pH range, and are the products of simple and reproducible chemical syntheses. 34,36 Although the determination of aptamer structure requires several steps, their in vitro synthesis is preferable to that of antibodies, which require synthesis in biological systems under highly specic conditions.…”

Development of a fluorescence assay for highly sensitive detection of Pseudomonas aeruginosa based on an aptamer-carbon dots/graphene oxide system

“…The LOD was an important parameter reecting the sensitivity of detection. 3,16 Previously, an aptamer-based FISH assay was established for detecting P. aeruginosa. However, the LOD was unclear.…”

“…1 In recent years, aptamer-based biosensors and assays have displayed greater advantages in detecting pathogenic microorganisms with rapidity and high specicity and sensitivity. 3 Aptamers are well known to be obtained through systematic evolution of ligands via exponential enrichment (SELEX). 4 Presently, high-throughput sequencing (HTS), which yields extensive data at the gigabyte level, can guarantee adequate sequence coverage, thereby enabling accurate recognition of the most abundant sequences in polymerase chain reaction (PCR) products in any round of SELEX; this provides a novel comprehensive method for precise identication of target aptamer.…”