Approaches for the detection of harmful algal blooms using oligonucleotide interactions

Bruce, Karen L.; Leterme, Sophie C.; Ellis, Amanda; Lenehan, Claire E.

doi:10.1007/s00216-014-8193-x

Cited by 14 publications

(8 citation statements)

References 106 publications

(171 reference statements)

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“…Damage to nervous or intestinal system rather than loss of memory have been observed depending on the type of algal bloom. Among them some dinoflagellate and diatom species, such as Noctiluca scintillans and Skeletonema costatum , are responsible only for discoloration of water and death of fish and other marine organisms, whereas species belonging to the genera Alexandrium, Gymnodinium, Dinophysis , and Pseudo-nitzschia are the main producers of marine biotoxins for humans ( Berti and Milandri, 2014 ; Bruce et al, 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Marine Biotoxins: Occurrence, Toxicity, Regulatory Limits and Reference Methods

et al. 2016

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Harmful algal blooms are natural phenomena caused by the massive growth of phytoplankton that may contain highly toxic chemicals, the so-called marine biotoxins causing illness and even death to both aquatic organisms and humans. Their occurrence has been increased in frequency and severity, suggesting a worldwide public health risk. Marine biotoxins can accumulate in bivalve molluscs and regulatory limits have been set for some classes according to European Union legislation. These compounds can be distinguished in water- and fat-soluble molecules. The first group involves those of Paralytic Shellfish Poisoning and Amnesic Shellfish Poisoning, whereas the toxins soluble in fat can cause Diarrheic Shellfish Poisoning and Neurotoxic Shellfish Poisoning. Due to the lack of long-term toxicity studies, establishing tolerable daily intakes for any of these marine biotoxins was not possible, but an acute reference dose can be considered more appropriate, because these molecules show an acute toxicity. Dietary exposure assessment is linked both to the levels of marine biotoxins present in bivalve molluscs and the portion that could be eaten by consumers. Symptoms may vary from a severe gastrointestinal intoxication with diarrhea, nausea, vomiting, and abdominal cramps to neurological disorders such as ataxia, dizziness, partial paralysis, and respiratory distress. The official method for the detection of marine biotoxins is the mouse bioassay (MBA) showing some limits due to ethical restrictions and insufficient specificity. For this reason, the liquid chromatography–mass spectrometry method has replaced MBA as the reference technique. However, the monitoring of algal blooms producing marine biotoxins should be regularly assessed in order to obtain more reliable, accurate estimates of bloom toxicity and their potential impacts.

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Section: Introductionmentioning

confidence: 99%

Marine Biotoxins: Occurrence, Toxicity, Regulatory Limits and Reference Methods

et al. 2016

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“…The increasing HAB events and the production by microscopic algae of dangerous biotoxins are responsible for diseases in humans, marine life and birds with an urgent request of monitoring programs and development of rapid and efficient diagnostic techniques. A comprehensive survey has been recently done on the molecular approaches used for monitoring the HAB events as well as on the progress in the use of oligonucleotide probes for the detection of multiple species in a single assay based on hybridization techniques [ 49 ]. Species-specific probes for the identification of microorganisms usually target the ribosomal RNA sequences.…”

Section: Discussionmentioning

confidence: 99%

Molecular Detection of a Potentially Toxic Diatom Species

Dhar

Cimarelli

Singh

et al. 2015

IJERPH

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A few diatom species produce toxins that affect human and animal health. Among these, members of the Pseudo-nitzschia genus were the first diatoms unambiguously identified as producer of domoic acid, a neurotoxin affecting molluscan shell-fish, birds, marine mammals, and humans. Evidence exists indicating the involvement of another diatom genus, Amphora, as a potential producer of domoic acid. We present a strategy for the detection of the diatom species Amphora coffeaeformis based on the development of species-specific oligonucleotide probes and their application in microarray hybridization experiments. This approach is based on the use of two marker genes highly conserved in all diatoms, but endowed with sufficient genetic divergence to discriminate diatoms at the species level. A region of approximately 450 bp of these previously unexplored marker genes, coding for elongation factor 1-a (eEF1-a) and silicic acid transporter (SIT), was used to design oligonucleotide probes that were tested for specificity in combination with the corresponding fluorescently labeled DNA targets. The results presented in this work suggest a possible use of this DNA chip technology for the selective detection of A. coffeaeformis in environmental settings where the presence of this potential toxin producer may represent a threat to human and animal health. In addition, the same basic approach can be adapted to a wider range of diatoms for the simultaneous detection of microorganisms used as biomarkers of different water quality levels.

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“…First step of LAMP mechanism: the non-cyclic reaction and formation of dual looped DNA strand. Modified from [14]. A) DNA synthesis initiated from FIP.…”

Section: Fig (2)mentioning

confidence: 99%

“…Second and third step of LAMP: the cyclic amplification and elongation leading to strand displacement DNA synthesis. Modified from [14]. Fig.…”

Section: Dna Extractionmentioning

confidence: 99%

Applying Loop-mediated Isothermal Amplification (LAMP) in the Diagnosis of Malaria, Leishmaniasis and Trypanosomiasis as Point-of-Care Tests (POCTs)

Dea-Ayuela

Galiana‐Roselló

Lalatsa

et al. 2018

CTMC

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One of the main objectives of the WHO is controlling transmission of parasitic protozoa vector-borne diseases. A quick and precise diagnosis is critical in selecting the optimal therapeutic regime that avoids unnecessary treatments and the emergence of resistance. Molecular assays based on loopmediated isothermal amplification (LAMP) techniques are a good alternative to light microscopy and antigen-based rapid diagnostic tests in developing countries, since they allow for a large amount of genetic material generated from a few copies of DNA, and use primers that lead to high sensitivity and specificity, while the amplification process can be performed in isothermal conditions without the need of sophisticated equipment to interpret the results. In this review, the main advances in the development of LAMP assays for the diagnosis of malaria, leishmaniasis and Chagas' disease are discussed as well as the feasibility of their implementation in developing countries and use as point-of-care diagnostic tests.

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Approaches for the detection of harmful algal blooms using oligonucleotide interactions

Cited by 14 publications

References 106 publications

Marine Biotoxins: Occurrence, Toxicity, Regulatory Limits and Reference Methods

Marine Biotoxins: Occurrence, Toxicity, Regulatory Limits and Reference Methods

Molecular Detection of a Potentially Toxic Diatom Species

Applying Loop-mediated Isothermal Amplification (LAMP) in the Diagnosis of Malaria, Leishmaniasis and Trypanosomiasis as Point-of-Care Tests (POCTs)

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