Approach to engineer tomato by expression of AtHMA4 to enhance Zn in the aerial parts

Kendziorek, Maria; Barabasz, Anna; Rudzka, Justyna; Tracz, Katarzyna; Mills, Rebecca F.; Williams, Lorraine E.; Antosiewicz, Danuta Maria

doi:10.1016/j.jplph.2014.04.017

Cited by 24 publications

(22 citation statements)

References 55 publications

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“…Tobacco plants (Nicotiana tabacum var. Xanthi) were grown in a growth chamber under the conditions described by Kendziorek et al (2014) Collected plant samples were immediately frozen in the liquid nitrogen and stored in - In parallel plants were grown under control conditions.…”

Section: Plant Material General Growth Conditions and Hydroponic Expmentioning

confidence: 99%

NtZIP11, a new Zn transporter specifically upregulated in tobacco leaves by toxic Zn level

Kozak

Papierniak

Barabasz

et al. 2019

Environmental and Experimental Botany

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Understanding the molecular mechanisms governing the uptake and accumulation of Zn in the leaves of tobacco plants exposed to high Zn concentrations is important from the perspective of phytoremediation of metal contaminated soil. This study identifies a new candidate gene, NtZIP11, which may contribute to Zn accumulation in tobacco leaves. NtZIP11 encodes a protein of 346 amino acids, with characteristic conserved sequences of the ZIP family of proteins. Phylogenetic analysis shows that NtZIP11 forms a distinct clade with other ZIP11 proteins. Transient expression of GFP-tagged ZIP11 in the abaxial epidermis of tobacco leaves demonstrates localization at the plasma membrane. Yeast complementation tests and growth assays indicate that NtZIP11 is involved in Zn but not Fe, Mn and Cd uptake. NtZIP11 complements the zrt1zrt2 mutant deficient in Zn uptake, but not the fet3fet4 mutant deficient in Fe uptake. Nor does it modify the sensitivity of wild-type yeast, DY1457, to increasing concentrations of Fe, Mn and Cd. NtZIP11 is expressed in both roots and leaves, with transcript abundance increasing with age. Noteworthy, the expression level of NtZIP11 is not modulated by Zndeficiency but is highly upregulated especially in the older leaves by high Zn concentrations (50 and 200 µM). Our data indicate that the primary role for NtZIP11 is in the uptake of Zn by the cells of tobacco leaves specifically when experiencing high Zn concentrations. It also likely contributes to maintaining a basal supply of Zn to cells at the level of the whole plant. Thus the present study contributes to broadening our understanding of Zn homeostasis mechanisms in tobacco, and clarifying the role of ZIP11 genes.

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Section: Plant Material General Growth Conditions and Hydroponic Expmentioning

confidence: 99%

NtZIP11, a new Zn transporter specifically upregulated in tobacco leaves by toxic Zn level

Kozak

Papierniak

Barabasz

et al. 2019

Environmental and Experimental Botany

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“…The medium was renewed every second day. The growth conditions applied were described by Kendziorek et al (2014).…”

Section: Methodsmentioning

confidence: 99%

“…RNA isolation, cDNA synthesis, and expression analysis were performed according to Kendziorek et al (2014) with minor modifications. The reaction was carried out in a volume of 15 µl, containing 0.2 µM primers and 6 µl of 80× diluted reaction mixture obtained after cDNA synthesis.…”

Section: Methodsmentioning

confidence: 99%

The ratio of Zn to Cd supply as a determinant of metal-homeostasis gene expression in tobacco and its modulation by overexpressing the metal exporter AtHMA4

Barabasz

Klimecka

Kendziorek

et al. 2016

EXBOTJ

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“…8). Plants exposed to high levels of metals such as Cu (Lequeux et al 2010, Mattos Jr et al 2010, Zn (Kendziorek et al 2014), nickel (Ni) (Kuhmar et al 2015) or cadmium (Cd) (Gratão et al 2015) has shown decreases in Fe levels of shoots/leaves and increase in roots. In this case, Fe is likely to become unavailable to plant growth, as confirmed by the reduction in the Fe-SOD activity of both rootstocks, even given the greater accumulation of nutrients in roots when plants were grown in the highest concentration of Cu (Fig.…”

Section: Copper and Iron Interactions In Citrus Treesmentioning

confidence: 99%

Photosynthesis is differently regulated during and after copper‐induced nutritional stress in citrus trees

Hippler

Dovis

Boaretto

et al. 2018

Physiologia Plantarum

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Antioxidant enzymatic responses in Citrus leaves under Cu-induced stress depends on rootstock genotypes. However, there is a lack of information about how woody plants recover growth capacity after exposure to elevated Cu and whether growth is affected by the redistribution of the metal to new vegetative parts and consequently whether photosynthesis is affected. Therefore, the biomass of plants and Cu concentrations in new leaf flushes were determined in young citrus trees grafted onto contrasting rootstocks [Swingle citrumelo (SW) and Rangpur lime (RL)]. Photosynthetic rate, chlorophyll fluorescence and antioxidant enzymatic systems were evaluated in plants previously grown in nutrient solution with Cu varying from low to high levels and with no added Cu. Both rootstocks exhibited reduced plant growth under Cu toxicity. However, trees grafted onto RL exhibited better growth recovery after Cu excess, which was dependent on the modulation of antioxidant enzyme activities in roots and leaves that maintained the integrity of the photosynthetic apparatus. In contrast, plants grafted onto SW exhibited a lower photosynthetic rate at the lowest available Cu concentration. Although the highest accumulation of Cu occurred in citrus roots, the redistribution of the nutrient to new vegetative parts was proportional to the Cu concentration in the roots.

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Approach to engineer tomato by expression of AtHMA4 to enhance Zn in the aerial parts

Cited by 24 publications

References 55 publications

NtZIP11, a new Zn transporter specifically upregulated in tobacco leaves by toxic Zn level

NtZIP11, a new Zn transporter specifically upregulated in tobacco leaves by toxic Zn level

The ratio of Zn to Cd supply as a determinant of metal-homeostasis gene expression in tobacco and its modulation by overexpressing the metal exporter AtHMA4

Photosynthesis is differently regulated during and after copper‐induced nutritional stress in citrus trees

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