Apport de la vitrification ovocytaire dans un laboratoire d’AMP. Vitrification des ovocytes : l’âge mûr

Boyer, Pierre; Montjean, Debbie; Tourame, P.; Boyer, M.

doi:10.1016/j.gyobfe.2013.07.021

Cited by 10 publications

(2 citation statements)

References 11 publications

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“…C'est dans cette indication que nous avons obtenu la validation méthodologique d'étude et de mise en pratique par un Comité de Protection des Personnes qui se prêtent aux recherches biomédicales (3 Avril 2008, référence 208 R06). L'étude avait permis d'inclure 87 femmes dans un délai de 9 mois, assurant un effectif suffisant pour l'évaluation interne (Boyer et al, 2014).…”

Section: Les Débuts En France En Amp Intraconjugale (Hors Don D'ovocyte)unclassified

“…Les données publiées en 2013 (Boyer et al, 2013(Boyer et al, , 2014 de la seule et unique validation française de la technique de vitrification ovocytaire étaient particulièrement encourageantes puisqu'elles montraient un taux de survie des ovocytes de 80,5 % vs. 90 % et plus en don et des taux de fécondation et de grossesse par transfert au moins égaux à ceux obtenus avec des ovocytes frais issus de la même cohorte. La maîtrise de la technique permet d'améliorer les taux de survie des ovocytes réchauffés à 86 %.…”

Section: Résultats De La Vitrification Ovocytaireunclassified

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La vitrification ovocytaire : un outil d’avenir

Boyer

Montjean

Siraudin

et al. 2015

Biologie Aujourd'hui

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Ovocyte vitrification was finally authorized by the new law voted in July 2011 upon the revision of the French bioethics law. Expected for 30 years, cryopreservation of female gametes had a major impact on Assisted Reproductive Technologies (ART) practice worldwide and in our country. It brought tremendous changes in the field of reproductive biology, from intraconjugal infertility management to gamete donation, through autologous cryopreservation for fertility preservation. Although it appears to be "obvious", ovocyte vitrification seems to be barely used as a routine technique in French IVF laboratories. We will discuss the events that led to the present situation. We will also tackle the expected benefits of ovocyte vitrification especially as an alternative to embryo freezing.

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Section: Les Débuts En France En Amp Intraconjugale (Hors Don D'ovocyte)unclassified

Section: Résultats De La Vitrification Ovocytaireunclassified

La vitrification ovocytaire : un outil d’avenir

Boyer

Montjean

Siraudin

et al. 2015

Biologie Aujourd'hui

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Chapter 1 Historical Background on Gamete and Embryo Cryopreservation

Ali

AlHarbi²,

Ali

2017

Methods in Molecular Biology

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This chapter describes the development of the science of cryopreservation of gametes and embryos of various species including human. It attempts to record in brief the main contributions of workers in their attempts to cryopreserve gametes and embryos. The initial difficulties faced and subsequent developments and triumphs leading to present-day state of the art are given in a concise manner. The main players and their contributions are mentioned and the authors' aim is to do justice to them. This work also attempts to ensure that credit is correctly attributed for significant advances in gamete and embryo cryopreservation. In general this chapter has tried to describe the historical development of the science of cryopreservation of gametes and embryos as accurately as possible without bias or partiality.

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Morphokinetics analysis of embryos derived from vitrified/warmed oocytes

Montjean

Geoffroy-Siraudin

Boyer

et al. 2015

J Assist Reprod Genet

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Purpose Oocyte vitrification is a worldwide used technique that has proved its worth. Although it was shown not to alter oocyte integrity, a recent study concluded that it may affect oocyte embryo development. As the morphology and kinetics of embryos derived from sibling fresh and vitrified oocytes have not been described previously, the present study evaluates cleavage rate, blastomeres size, fragmentation rate, and blastocyst formation in vitrified/warmed oocyte derived embryos (VODE) as compared with sibling fresh oocytes derived embryos (FODE). Methods This investigation included 90 infertility cases displaying large cohort of mature oocytes at pick up, divided into 2 groups after denudation. A part of oocytes underwent ICSI while others were vitrified. Oocyte warming cycles were performed when no pregnancy was achieved using fresh eggs. Zygote to blastocyst development was recorded prospectively in an image database up to day 5. Results VODE did not show major difference as compared with FODE in terms of cleavage rate, number of blastomeres, fragmentation rate, and blastomeres size. Furthermore, percentage of morulae at day 4 and blastocysts at day 5 are not affected by oocyte vitrification. Conclusion Although our results show that embryo development is not altered by oocyte vitrification, offspring follow-up is essential to exclude any adverse developmental effect of the technique.

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Apport de la vitrification ovocytaire dans un laboratoire d’AMP. Vitrification des ovocytes : l’âge mûr

Cited by 10 publications

References 11 publications

La vitrification ovocytaire : un outil d’avenir

La vitrification ovocytaire : un outil d’avenir

Chapter 1 Historical Background on Gamete and Embryo Cryopreservation

Morphokinetics analysis of embryos derived from vitrified/warmed oocytes

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