Applying proteomics to detect early signs of chronic kidney disease: where has the magic gone?

Klein, Jon B.

doi:10.1080/14789450.2017.1315303

Cited by 4 publications

(2 citation statements)

References 23 publications

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“…Notably, our results were further supported by transcriptomic data obtained from human kidney biopsies [15] and were validated in additional spontaneous and induced SLE mouse models. To our knowledge these proteins have not been identified previously in proteomics studies in other kidney diseases [39,40].…”

Section: Discussionmentioning

confidence: 83%

Proteomic analysis in lupus mice identifies Coronin-1A as a potential biomarker for lupus nephritis

et al. 2020

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Background: Approximately 50% of systemic lupus erythematosus (SLE) patients develop nephritis, which is among the most severe and frequent complications of the disease and a leading cause of morbidity and mortality. Despite intensive research, there are still no reliable lupus nephritis (LN) markers in clinical use that can assess renal damage and activity with a high sensitivity and specificity. To this end, the aim of this study was to identify new clinically relevant tissue-specific protein biomarkers and possible underlying molecular mechanisms associated with renal involvement in SLE, using mass spectrometry (MS)-based proteomics. Methods: Kidneys were harvested from female triple congenic B6.NZMsle1/sle2/sle3 lupus mice model, and the respective sex-and age-matched C57BL/6 control mice at 12, 24 and 36 weeks of age, representing presymptomatic, established and end-stage LN, respectively. Proteins were extracted from kidneys, purified, reduced, alkylated and digested by trypsin. Purified peptides were separated by liquid chromatography and analysed by high-resolution MS. Data were processed by the Progenesis QIp software, and functional annotation analysis was performed using DAVID bioinformatics resources. Immunofluorescence and multiple reaction monitoring (MRM) MS methods were used to confirm prospective biomarkers in SLE mouse strains as well as human serum samples.

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Section: Discussionmentioning

confidence: 83%

Proteomic analysis in lupus mice identifies Coronin-1A as a potential biomarker for lupus nephritis

et al. 2020

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“…Proteomic studies have produced less rapid success in this area than was initially predicted a decade ago 8 due to multiple challenges such as the complexity of proteomics technology; strict requirements for standardized specimen collection, processing and storage; difficulties in quantification of urinary markers with low abundances; and the large variation in urine protein excretion between individuals. Despite these difficulties, a urinary biomarker panel was derived from capillary electrophoresis-mass spectrometry (MS) analysis of urine samples of patients with CKD.…”

Section: Systems Biological Approaches To Develop New Dkd Biomarkersmentioning

confidence: 99%

The Promise of Systems Biology for Diabetic Kidney Disease

Brosius

2018

Advances in Chronic Kidney Disease

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Diabetic kidney disease (DKD) has a complex and prolonged pathogenesis involving many cell types in the kidney as well as extrarenal factors. It is clinically silent for many years after the onset of diabetes and usually progresses over decades. Given this complexity, a comprehensive and unbiased molecular approach is best suited to help identify the most critical mechanisms responsible for progression of DKD and those most suited for targeted intervention. Systems biological investigations provide such an approach since they examine the entire network of molecular changes that occur in a disease process in a comprehensive way instead of focusing on a single abnormal molecule or pathway. Systems biological studies can also start with analysis of the disease in humans, not in animal or cell culture models that often poorly reproduce the changes in human DKD. Indeed, in the last decade, systems biological approaches have led to the identification of critical molecular abnormalities in DKD and have directly led to development of new biomarkers and potential treatments for DKD.

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Quantification of molecular heterogeneity in kidney tissue by targeted proteomics

Hoyer

Dittrich

Bartram

et al. 2019

Journal of Proteomics

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Applying proteomics to detect early signs of chronic kidney disease: where has the magic gone?

Cited by 4 publications

References 23 publications

Proteomic analysis in lupus mice identifies Coronin-1A as a potential biomarker for lupus nephritis

Proteomic analysis in lupus mice identifies Coronin-1A as a potential biomarker for lupus nephritis

The Promise of Systems Biology for Diabetic Kidney Disease

Quantification of molecular heterogeneity in kidney tissue by targeted proteomics

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