Applying fluorescence spectroscopy and multivariable analysis to characterize structural composition of dissolved organic matter and its correlation with water quality in an urban river

Abstract

Excitation-emission matrix fluorescence spectroscopy combined with multivariable analysis was employed to discriminate structural component of dissolved organic matter (DOM) in Xihe River, an urban river, located in western Shenyang City of China, and to reveal its correlation with water quality. Eight water samples were collected along the river across the centralized wastewater-discharge region and the dispersed wastewater-discharge region. Water quality had deeply deteriorated in the river. Based on DOM fluorescence spectroscopic properties, DOM was composed of tyrosine-like, tryptophan-like, soluble microbial by-product-like, fulvic-like and humic-like materials, among which protein-like material was dominant in the DOM fractions. The DOM in the centralized wastewater-discharge region had a lower level of aromaticity than that in the dispersed wastewaterdischarge region, and its humification degree was lower than that in the latter too. Key factors for the trophic state contained NH 4 -N, DO, DOC, TP, U 2,n and U 4,n by multivariable analysis. DOM was mainly derived from treated and untreated industrial wastewater with weak biodegradability.

Characterization of CDOM of river waters in China using fluorescence excitation‐emission matrix and regional integration techniques

“…FRI has been widely used to quantitatively analyze and study the EEM fluorescence characteristics of CDOM [Chen et al, 2003;He et al, 2013;Wang et al, 2009]. As shown in Figure 3 and Table 2a, FRI divides EEM into five regions, each representing a CDOM fluorescence fraction: the tyrosine-like (R1), tryptophan-like (R2), microbial protein-like (R4), fulvic-like (R3), and humic-like (R5) fluorescent components [Chen et al, 2013;Yu et al, 2015]. The EEM spectrum varied with the intensities of the five marked fluorescence fractions for various types of river water samples.…”

“…Chai et al [2003] used EEM-FRI to investigate the composition and transformation of humic and fulvic acids from landfill. Yu et al [2015] also divided the EEM spectra into five defined regions and investigated the correlations between FRI-divided FDOM components and water quality in an urban river. Due to the FDOM components in river waters generally refractory and labile with varying environments (i.e., hydrologic, geographical, climatic, and landscape characteristics), it is important to investigate CDOM sources using EEM-FRI method in river waters for different river basins across China.…”

Urban stream Osówka in Szczecin – how efficiently as possible should the variability of the stream water quality be studied along the road of runoff in time?

“…While such changes would prevent the conduction of studies on the biohydrogeochemical processes taking place in examined waters eg. equilibria of processes of organic matter mineralization (Yu et al 2015). or balance processes between the forms of nitrogen (Lusk.…”

Relationships Between DOC and CDOM Based on the Total Carbon‐Specific Fluorescence Intensities for River Waters Across China

“…Compared with the peak‐picking method and EEM‐PARAFAC method, EEM‐FRI, as a quantitative technique, is associated with all wavelength‐dependent paired fluorescence intensity data in an EEM. Therefore, EEM‐FRI method can be used to investigate the dynamic of FDOM regions for river waters with site‐specific optical properties due to different hydrologic, geographical, climatic, and landscape characteristics, physical/chemical properties, land use/cover, soil type, and pollution (Yu et al, ; Zhao et al, ).…”