Applied photochemistry

Kagan, Jacques

doi:10.1016/b978-0-12-394320-0.50015-6

Cited by 7 publications

(1 citation statement)

References 42 publications

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“…A sequence motif identical to the chi element GCTGGTGG and a chi-like motif, with only one base substitution, TCTGGTGG, were found 28 and 10 nucleotides downstream and upstream to the breakpoint in intron 7 of FUS. In case L1686, the TLS737F-TLS918R CHOP31F-CHOP176R TLS737F-CHOP176R TLS737F-CHOP176R TLS737F-CHOP176R TLS737F-ERG837R EWS685F-CHOP176R TLS816F-TLS844R CHOP53F-CHOP105R TLS816F-CHOP105R TLS816F-CHOP105R TLS816F-CHOP105R TLS816F- ERG837R ERG810R EWS685F EWS223F TLS737F TLS816F TLS918R TLS844R CHOP31F CHOP53F CHOP105R CHOP176R , 1989;Kagan, 1993;Showe and Croce, 1987), the participating protooncogene did not always contain such motifs (Adachi and Tsujimoto, 1990;Boehm and Rabbitts, 1989;Kasai et al, 1992). A 45 Kd protein was reported to bind a homopurinehomopyrimidine present in the major breakpoint region of the BCL2 and in the IgH locus (Jaeger et al, 1993), suggesting a recombination mechanism distinct from that of the IG rearrangement.…”

Section: Resultsmentioning

confidence: 99%

Characteristic sequence motifs at the breakpoints of the hybrid genes FUS/CHOP, EWS/CHOP and FUS/ERG in myxoid liposarcoma and acute myeloid leukemia

et al. 1997

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We have sequenced the breakpoint regions in one acute myeloid leukemia (AML) with t(16;21)(p11;q22) resulting in the formation of a FUS/ERG hybrid gene and in four myxoid liposarcomas (MLS), three of which had the translocation t(12;16) (q13;p11) and a FUS/CHOP fusion gene and one with t(12;22;20)(q13;q12;q11) and an EWS/CHOP hybrid gene. The breakpoints were localized to intron 7 of FUS, intron 1 of CHOP, an intronic sequence of ERG and intron 7 of EWS. In two MLS cases with t(12;16) and in the AML, the breaks in intron 7 of FUS had occurred close to each other, a few nucleotides downstream from a TG dinucleotide repeat region. The break in the two MLS had occurred in the same ATGGTG hexamer and in the AML 40 nucleotides upstream from the hexamer. The third case of t(12;16) MLS had a break upstream and near a TCdinucleotide repeat region and a sequence similar to the chi bacterial recombination element was found tō ank the breakpoint. In the MLS with the EWS/ CHOP hybrid gene, the break in intron 7 of EWS had occurred close to an Alu sequence. Similarly, in all 4 MLS, the breaks in intron 1 of CHOP were near an Alu sequence. No Alu or other repetitive sequences were found 250 bp upstream or downstream from the break in the ERG intron involved in the AML case. In the AML, the MLS with ESW/CHOP and in one MLS with FUS/CHOP there were one, two and six, respectively, nucleotide identity between the contributing germline sequences in the breakpoint. In the other two MLS cases, two and three extra nucleotides of unknown origin were inserted between the FUS and CHOP sequences. At the junction and/or in its close vicinity, identical oligomers, frequently containing a trinucleotide TGG, were found in both partner genes. Our data thus show that all four genes-FUS, EWS, CHOP and ERG-contain characteristic motifs in the breakpoint regions which may serve as speci®c recognition sites for DNA-binding proteins and have functional importance in the recombination events taking place between the chromosomes. Di erent sequence motifs may, however, play a role in each individual case.

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Section: Resultsmentioning

confidence: 99%