Applications of lipid nanoparticles in gene therapy

Gene delivery to the rat retina by non-viral vectors based on chloroquine-containing cationic niosomes

“…Compared to subretinal injection, intravitreal injection represents an interesting alternative to deliver genetic material to the back of the eye, and therefore to access retinal structure. It is a less invasive route, more easily to perform, and higher doses can be delivered [46]. Consequently, large retinal surfaces can be transfected by this route of administration [47].…”

Gene Therapy in Cancer Treatment: Why Go Nano?

“…SLN is a solid lipid capped by a layer of surfactants in aqueous dispersion. There are numerous synthesis procedures, the most common used is high pressure homogenization [153,220]. To promote the functionalization with the genetic material several types of surfactants might be used.…”

“…Lipids nanoparticles charged with drugs or a condensed DNA core surrounded by lipid bilayers have been applied in retinitis pigmentosa or age-related macular degeneration [221]. The combination of DNA delivery using SLN and drugs was proposed as a promising strategy for cancer therapy [153]. Penumarthi and collaborators developed a SLN conjugated with DNA and showed the biocompatibility and the high transfection rate in dendritic cells [224].…”

Introduction to the Inaugural Issue of Journal of Cancer Diagnosis

“…At the moment, we must highlight many advances: a) liquid biopsy: formalin fixation and paraffin embedding, which had long been the default pathological biopsy medium, has now being supplemented with liquid biopsy, a way to profile the cancer genomes of patients; b) nanotechnology in diagnosis and treatment of cancer; for example, many authors have focused on the development of miRNA nanoformulations to achieve accumulation at the tumor site [11]; another authors have focused on lipid nanoparticles, a non-viral vectors for gene transfection, as an effective and safety alternative to potentially treat different diseases, such as cancer [12]; and c) cancer stem cells resistance to treatment [13].…”