Modern electronics products must have improved characteristics, including high-speed, high-density, and lowervoltage operations. With such designs, a poor PCB (printed circuit board) layout in the signal integrity (SI) system is affected by the noise and becomes unstable. Crosstalk is a major noise that interferes with SI. Generally, a guard trace is added between Victim and the other the Aggressor to prevent crosstalk. Two grounded vias are added between two ends of this guard trace, respectively, for reducing crosstalk. With the working frequency being higher and higher, crosstalk interferes more and more serious. Even, this guard trace will be as one noise source to affect Victim. In this paper, we present the effects of a guard trace with the optimal number of grounded vias that gives the maximum efficiency for preventing crosstalk in parallel double micro-strip lines in a high-speed PCB layout. In comparison with a guard trace with two terminal grounded vias, our swing of the near-end crosstalk is its 37%, and our swing of far-end crosstalk is its 45%.