Applications of Immobilised Boronic Acids

Dean, P.D.G.; Middle, Fiona A.; Longstaff, Colin; Bannister, Anne; Dembinski, Jadwiga J.

doi:10.1016/b978-0-12-166580-7.50068-2

Cited by 5 publications

(1 citation statement)

References 55 publications

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“…Immobilized phenylboronates (Dean et al, 1983) were investigated as a means of overcoming the problems of using free phenylboric acid. In this approach phenylboronate ligands are covalently attached to a support (usually agarose or polyacrylamide).…”

Section: Recovery Using Immobilized Boronatesmentioning

confidence: 99%

Boron based separations for in situ recovery of L-erythrulose from transketolase-catalyzed condensation

Chauhan

Powell

Woodley

1997

Biotechnol. Bioeng.

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In this article we report on the application of in situ product removal (ISPR) (the concurrent recovery of a product during the product formation process) as a means of improving the productivity of bioconversions. The Escherichia coli transketolase‐catalyzed condensation of glycolaldehyde with β‐hydroxypyruvate to yield L‐erythrulose (and carbon dioxide) was chosen as a model system. Those ISPR methods based on phenylboronate‐diol interactions showed greatest potential for use as a selective means of removing L‐erythrulose from the reaction medium. Soluble, insoluble, and immobilized boronates were investigated. Concentrations of free phenylboric acid of 100 mM and above were toxic to transketolase, thus rendering the use of these methods unsuitable for ISPR. However, one of the immobilized phenylboronate resins (Affi‐Gel 601®) was not toxic to the enzyme, although significant levels of nonspecific binding of both substrates were observed. When ISPR was performed on the model reaction using this resin with substrate feeding, it proceeded to completion. © 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 56: 345–351, 1997.

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Section: Recovery Using Immobilized Boronatesmentioning

confidence: 99%

Boron based separations for in situ recovery of L-erythrulose from transketolase-catalyzed condensation

Chauhan

Powell

Woodley

1997

Biotechnol. Bioeng.

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Affinity Chromatography

Batista‐Viera¹,

Janson²,

Carlsson³

2011

Methods of Biochemical Analysis

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Two types of resin-conjugated Tamiflu analogs were synthesized by modifying our original synthetic route of oseltamivir phosphate (Tamiflu). The prepared resins bound to influenza virus neuraminidase, the main target of Tamiflu. The resins will be useful for isolating and identifying presumed endogenous vertebrate proteins that interact with Tamiflu, which might relate to the rarely observed abnormal behavior exhibited by some influenza patients treated with Tamiflu.

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