Applications of genomic technologies to the study of organochlorine pesticide-induced reproductive toxicity in fish

García-Reyero, Natàlia; Denslow, Nancy D.

doi:10.1584/jpestics.31.252

Cited by 16 publications

(5 citation statements)

References 123 publications

(116 reference statements)

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“…The biological responses to external stressors, including toxicants, involve changes in normal patterns of gene expression [67,68]. Many responses are a direct result of the chemical, such as alterations in gene expression caused by the binding of a steroid hormone (or analogue) to a specific steroid hormone receptor, which acts as a TF and subsequently modulates (activates or represses) the transcription of its target genes.…”

Section: The Elucidation Of the Signaling Pathways And Transcription mentioning

confidence: 99%

Endocrine Disruption in the Omics Era: New Views, New Hazards, New Approaches

Oliveira¹,

Barata²,

Piña³

2016

TOBIOTJ

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Abstract:The genome revolution has brought about a complete change on our view of biological systems. The quantitative determination of changes in all the major molecular components of the living cells, the "omics" approach, opened whole new fields for all health sciences, including toxicology. Endocrine disruption, i.e., the capacity of anthropogenic pollutants to alter the hormonal balance of the organisms, is one of the fields of Ecotoxicology in which omics has a relevant role. In the first place, the discovery of scores of potential targets in the genome of almost any Metazoan species studied so far, each of them being a putative candidate for interaction with endocrine disruptors. In addition, the understanding that ligands, receptors, and their physiological functions suffered fundamental variations during animal evolution makes it necessary to assess disruption effects separately for each major taxon. Fortunately, the same deal of knowledge on genes and genomes powered the development of new high-throughput techniques and holistic approaches. Genomics, transcriptomics, proteomics, metabolomics, and others, together with appropriate prediction and modeling tools, will mark the future of endocrine disruption assessment both for wildlife and humans.

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Section: The Elucidation Of the Signaling Pathways And Transcription mentioning

confidence: 99%

Endocrine Disruption in the Omics Era: New Views, New Hazards, New Approaches

Oliveira¹,

Barata²,

Piña³

2016

TOBIOTJ

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“…While none of the genes were specific for a single individual exposure, a specific gene expression pattern was induced by each exposure. This exposure pattern is known as a compound signature and is being increasingly applied in ecotoxicology and risk assessment – .…”

Section: Resultsmentioning

confidence: 99%

Biomarker Discovery and Transcriptomic Responses in Daphnia magna exposed to Munitions Constituents

García-Reyero

Poynton

Kennedy

et al. 2009

Environ. Sci. Technol.

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Ecotoxicogenomic approaches are emerging as alternative methods in environmental monitoring because they allow insight into pollutant modes of action and help assess the causal agents and potential toxicity beyond the traditional end points of death, growth, and reproduction. Gene expression analysis has shown particular promise for identifying gene expression biomarkers of chemical exposure that can be further used to monitor specific chemical exposures in the environment. We focused on the development of gene expression markers to detect and discriminate between chemical exposures. Using a custom cDNA microarray for Daphnia magna, we identified distinct expression fingerprints in response to exposure at sublethal concentrations of Cu, Zn, Pb, and munitions constituents. Using the results obtained from microarray analysis, we chose a suite of potential biomarkers for each of the specific exposures. The selected potential biomarkers were tested in independent chemical exposures for specificity using quantitative reverse transcription polymerase chain reaction. Six genes were confirmed as differentially regulated bythe selected chemical exposures. Furthermore, each exposure was identified by response of a unique combination (suite) of individual gene expression biomarkers. These results demonstrate the potential for discovery and validation of novel biomarkers of chemical exposures using gene expression analysis, which could have broad applicability in environmental monitoring.

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Section: The Elucidation Of the Signaling Pathways And Transcription mentioning

confidence: 99%

Endocrine Disruption in the Omics Era: New Views, New Hazards, New Approaches

Oliveira¹,

Barata²,

Piña³

2015

TOBIOTJ

View full text Add to dashboard Cite

The genome revolution has brought about a complete change on our view of biological systems.The quantitative determination of changes in all the major molecular components of the living cells, the "omics" approach, opened whole new fields for all health sciences, including toxicology. Endocrine disruption, i.e., the capacity of anthropogenic pollutants to alter the hormonal balance of the organisms, is one of the fields of Ecotoxicology in which omics has a relevant role. In the first place, the discovery of scores of potential targets in the genome of almost any Metazoan species studied so far, each of them being a putative candidate for interaction with endocrine disruptors. In addition, the understanding that ligands, receptors, and their physiological functions suffered fundamental variations during animal evolution makes it necessary to assess disruption effects separately for each major taxon. Fortunately, the same deal of knowledge on genes and genomes powered the development of new high-throughput techniques and holistic approaches. Genomics, transcriptomics, proteomics, metabolomics, and others, together with appropriate prediction and modeling tools, will mark the future of endocrine disruption assessment both for wildlife and humans.

show abstract

Applications of genomic technologies to the study of organochlorine pesticide-induced reproductive toxicity in fish

Cited by 16 publications

References 123 publications

Endocrine Disruption in the Omics Era: New Views, New Hazards, New Approaches

Endocrine Disruption in the Omics Era: New Views, New Hazards, New Approaches

Biomarker Discovery and Transcriptomic Responses in Daphnia magna exposed to Munitions Constituents

Endocrine Disruption in the Omics Era: New Views, New Hazards, New Approaches

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