Applications of electrostatic stretch-and-positioning of DNA

Washizu, Masao; Kurosawa, Osamu; Arai, I.; Suzuki, Seiichi; Shimamoto, Nobuo

doi:10.1109/28.382102

Cited by 147 publications

(114 citation statements)

References 10 publications

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“…This interpretation is supported by the nearly steady decrease in measured ͑d / dt͒C. Whether the reported adhesion of DNA to aluminum 33 leads to a decreased ͑d / dt͒C by adhesion to the electrode surface, especially to that of the inactive electrodes, or to a rise of the apparent response due to adhesion exactly at the edges of the active electrodes where the influence on capacitance should be strongest is presently unclear. Purpose built electrodes with a less adsorbing surface and a smaller inactive area could help to clarify this question.…”

Section: Resultsmentioning

confidence: 89%

Dielectrophoresis of DNA: Quantification by impedance measurements

2010

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Dielectrophoretic properties of DNA have been determined by measuring capacitance changes between planar microelectrodes. DNA sizes ranged from 100 bp to 48 kbp, DNA concentrations from below 0.1 to 70 g / ml. Dielectrophoretic spectra exhibited maximum response around 3 kHz and 3 MHz. The strongest response was found for very long DNA ͑above 10 kbp͒ and for short 100 bp fragments, which corresponds to the persistence length of DNA. The method allows for an uncomplicated, automatic acquisition of the dielectrophoretic properties of submicroscopical objects without the need for labeling protocols or optical accessibility.

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Section: Resultsmentioning

confidence: 89%

Dielectrophoresis of DNA: Quantification by impedance measurements

2010

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“…It was also shown that fragments of different lengths could be sorted into ascending order of size, stretched across an inter-electrode gap. Later work [Washizu et al 1994a] demonstrated the same technique with smaller DNA fragments and a laser, and to collect the cut fragments. This work demonstrated that the manipulation of single molecular-scale objects (albeit relatively large molecules) could be manipulated and, in conjunction with the same group's work on proteins [Washizu et al 1994], formed the foundations of sub-micrometre dielectrophoresis research.…”

Section: Manipulation Of Single Nanoparticlesmentioning

confidence: 99%

AC electrokinetics: applications for nanotechnology

Hughes¹

2000

Nanotechnology

266

177

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The phenomena of dielectrophoresis and electrorotation, collectively referred to as AC electrokinetics, have been used for many years to study, manipulate and separate particles on the nanotechnology, that is the precise manipulation of particles on the nanometre scale. In this paper we present the principles of AC electrokinetics for particle manipulation, review the current state of AC Electrokinetic techniques for the manipulation of particles on the nanometre scale, and consider how these principles may be applied to nanotechnology.3

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“…Many of these techniques are relatively time consuming, costly and often lead to significant loss of the analyte. AC electrokinetic techniques, like dielectrophoresis (DEP) have been attractive because they allow cells [9][10][11], hmw-DNA biomarkers [12][13][14][15] and proteins [16] to be rapidly isolated and concentrated into specific microscopic locations. Dielectrophoresis (DEP) is an induced motion of particles produced by the dielectric differences between the particles and media in an AC electric field [17,18].…”

Section: Introductionmentioning

confidence: 99%

An AC electrokinetic method for enhanced detection of DNA nanoparticles

Krishnan

Heller

2009

Journal of Biophotonics

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Journal of BIOPHOTONICSIn biomedical research and diagnostics it is a challenge to isolate and detect low levels of nanoparticles and nanoscale biomarkers in blood and other biological samples. While highly sensitive epifluorescent microscope systems are available for ultra low level detection, the isolation of the specific entities from large sample volumes is often the bigger limitation. AC electrokinetic techniques like dielectrophoresis (DEP) offer an attractive mechanism for specifically concentrating nanoparticles into microscopic locations. Unfortunately, DEP requires significant sample dilution thus making the technology unsuitable for biological applications. Using a microelectrode array device, special conditions have been found for the separation of hmw-DNA and nanoparticles under high conductance (ionic strength) conditions. At AC frequencies in the 3000-10 000 Hz range, 10 mm microspheres and human T lymphocytes can be isolated into the DEP low field regions, while hmw-DNA and nanoparticles can be concentrated into microscopic high field regions for subsequent detection using an epifluorescent system.Final separation and detection of 150 ng/mL OliGreen fluorescent stained high molecular weight (hmw) DNA in 1 Â TBE buffer (109 mS/m). The DEP field was applied at 10 000 Hz and 10 volts pk-pk for 5 minutes to group of nine microelectrodes. The green fluorescent image (Ex 505 nm, Em 520 nm) shows fluorescence from the OliGreen stained hmw DNA concentrated on the 80-micron diameter microelectrodes with the far right column of unactivated microelectrodes showing no concentration of hmw-DNA.

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Applications of electrostatic stretch-and-positioning of DNA

Cited by 147 publications

References 10 publications

Dielectrophoresis of DNA: Quantification by impedance measurements

Dielectrophoresis of DNA: Quantification by impedance measurements

AC electrokinetics: applications for nanotechnology

An AC electrokinetic method for enhanced detection of DNA nanoparticles

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