Applications of CRISPR/Cas9-based genome editing in the plant biology

Zheng, Yang; Li, Qianqian; Ye, Mingwang; Chen, Aie; Wang, Hongyang

doi:10.3906/bot-2103-50

Cited by 9 publications

(5 citation statements)

References 109 publications

(115 reference statements)

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“…CRISPR-Cas9-mediated gene-editing tools, including PE, have been widely applied in plant cells [ 24 ], to increase yield, regulate metabolites, improve stress resistance, etc. [ 25 ].…”

Section: Discussionmentioning

confidence: 99%

Development and Optimization of CRISPR Prime Editing System in Photoautotrophic Cells

et al. 2022

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Prime editor (PE), a versatile editor that allows the insertion and deletion of arbitrary sequences, and all 12-point mutations without double-strand breaks (DSB) and a donor template, dramatically enhances research capabilities. PE combines nickase Cas9(H840A) and reverse transcriptase (RT), along with prime editing guide RNA (pegRNA). It has been reported in several plant species, but a weak editing efficiency has led to a decrease in applications. This study reports an optimized-prime editor (O-PE) for endogenous gene editing in Arabidopsis thaliana cells, with an average 1.15% editing efficiency, which is 16.4-fold higher than previously reported. Meanwhile, we observed an increase in indels when testing alternative reverse transcriptase and found out that nCas9(H840A) fused to non-functional reverse transcriptase was responsible for the increase. This work develops an efficient prime editor for plant cells and provides a blueprint for applying PE in other photoautotrophic cells, such as microalgae, that have a high industrial value.

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“…CRISPR-Cas9-mediated gene-editing tools, including PE, have been widely applied in plant cells [ 24 ], to increase yield, regulate metabolites, improve stress resistance, etc. [ 25 ].…”

Section: Discussionmentioning

confidence: 99%

Development and Optimization of CRISPR Prime Editing System in Photoautotrophic Cells

et al. 2022

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“…Krishna et al [ 104 ] further highlight CRISPR/Cas9′s role in conferring molecular immunity against drought stress in tomato, underlining its effectiveness in bolstering plant resilience. The versatility of CRISPR/Cas9 is also evident in the research of Zheng et al [ 105 ], who demonstrated its application in enhancing stress resistance across different plant species, thereby showcasing its broad applicability in plant stress management. Moreover, Park et al [ 106 ] presented evidence of CRISPR/Cas9′s capability to improve both abiotic and biotic stress tolerance in rice, underscoring its potential as a comprehensive tool for enhancing plant resilience to a wide array of environmental challenges, including drought.…”

Section: Crispr-cas9 and Genome Editingmentioning

confidence: 98%

Advanced Biotechnological Interventions in Mitigating Drought Stress in Plants

Şimşek,

Isak,

Dönmez

et al. 2024

Plants

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This comprehensive article critically analyzes the advanced biotechnological strategies to mitigate plant drought stress. It encompasses an in-depth exploration of the latest developments in plant genomics, proteomics, and metabolomics, shedding light on the complex molecular mechanisms that plants employ to combat drought stress. The study also emphasizes the significant advancements in genetic engineering techniques, particularly CRISPR-Cas9 genome editing, which have revolutionized the creation of drought-resistant crop varieties. Furthermore, the article explores microbial biotechnology’s pivotal role, such as plant growth-promoting rhizobacteria (PGPR) and mycorrhizae, in enhancing plant resilience against drought conditions. The integration of these cutting-edge biotechnological interventions with traditional breeding methods is presented as a holistic approach for fortifying crops against drought stress. This integration addresses immediate agricultural needs and contributes significantly to sustainable agriculture, ensuring food security in the face of escalating climate change challenges.

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“…Scope of CRISPR In the field of plant biology, the initial application of CRISPR/Cas9-based genome editing (Zheng et al, 2021). Demonstrated its extensive adaptability in the model species Arabidopsis and in the staple crop rice.…”

Section: Crispr/ Cas Genementioning

confidence: 99%

Battling arid adversity: unveiling the resilience of cotton in the face of drought and innovative mitigation approaches

BIBI,

LIHONG,

MAHMOOD

et al. 2024

Not Bot Horti Agrobo

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Climate change has had significant impacts on agriculture, particularly on cotton production, where drought has emerged as a major threat worldwide. Long and intense dry periods in cotton-growing regions have become more frequent and severe. Drought stress severely affects various aspects of cotton plants, including chlorophyll pigments, carbohydrate metabolism, and enzyme activities related to fiber development, such as vacuolar invertase and sucrose synthase. Furthermore, drought stress disrupts the movement of nutrients toward the reproductive tissues in cotton, resulting in compromised pollen function, propagative failure, and fiber characteristics. To tackle these issues, scientists have made advancements in creating drought-resistant cotton varieties through transgenic methods or molecular breeding techniques, genome editing, CRISPR/Cas9, utilizing quantitative trait loci (QTL). Moreover, the application of plant growth regulators and mineral elements has displayed the potential to improve cotton’s ability to endure drought stress while also enhancing fiber yield and quality. These approaches activate stress-responsive signaling pathways, which could contribute to mitigating reproductive failure and improving fiber characteristics. While the impact of drought stress on cotton plants has been extensively studied, the variations in fiber quality resulting from drought stress are not yet completely understood. Current research has been focused on unraveling the mechanisms underlying these changes, including the physiological, biochemical, and molecular alterations during the multiplicative growth phase that contribute to poor fiber development. Understanding these mechanisms will facilitate the development of novel strategies to alleviate the adverse impact of worldwide weather changes on cotton growth and fiber quality. This research focuses on the drought stress in cotton cultivation and explores its different effects on cotton morphology, physiology, crop yield, and fiber characteristics as well as mechanisms by which cotton exhibits drought tolerance and highlights innovative strategies to mitigate drought stress.

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Applications of CRISPR/Cas9-based genome editing in the plant biology

Cited by 9 publications

References 109 publications

Development and Optimization of CRISPR Prime Editing System in Photoautotrophic Cells

Development and Optimization of CRISPR Prime Editing System in Photoautotrophic Cells

Advanced Biotechnological Interventions in Mitigating Drought Stress in Plants

Battling arid adversity: unveiling the resilience of cotton in the face of drought and innovative mitigation approaches

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