Applications of capillary electrophoresis with chemiluminescence detection in clinical, environmental and food analysis. A review

Lara, Francisco J.; Airado‐Rodríguez, Diego; Moreno‐González, David; Huertas-Pérez, José F.; Garcı́a-Campaña, Ana M.

doi:10.1016/j.aca.2016.01.046

Cited by 61 publications

(19 citation statements)

References 119 publications

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“…Optical labels such as colorimetric nanoparticles (85)(86)(87), fluorescent tags (88), and chemiluminescent labels (89) are increasingly being used for DNA hybridization assays. However, due to simple instrumentation, increased sensitivity, and low background, chemiluminescent label-based techniques are preferred over fluorescence-based detection (90,91). Because of their easy manipulation under an external magnetic field, surface-modified magnetic particles can be used for enrichment of the target molecules, and this permits high-throughput and automated detection platforms (92)(93)(94)(95).…”

Section: Nucleic Acid-based Testsmentioning

confidence: 99%

Virological and Immunological Outcomes of Coinfections

et al. 2018

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SUMMARYCoinfections involving viruses are being recognized to influence the disease pattern that occurs relative to that with single infection. Classically, we usually think of a clinical syndrome as the consequence of infection by a single virus that is isolated from clinical specimens. However, this biased laboratory approach omits detection of additional agents that could be contributing to the clinical outcome, including novel agents not usually considered pathogens. The presence of an additional agent may also interfere with the targeted isolation of a known virus. Viral interference, a phenomenon where one virus competitively suppresses replication of other coinfecting viruses, is the most common outcome of viral coinfections. In addition, coinfections can modulate virus virulence and cell death, thereby altering disease severity and epidemiology. Immunity to primary virus infection can also modulate immune responses to subsequent secondary infections. In this review, various virological mechanisms that determine viral persistence/exclusion during coinfections are discussed, and insights into the isolation/detection of multiple viruses are provided. We also discuss features of heterologous infections that impact the pattern of immune responsiveness that develops.

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Section: Nucleic Acid-based Testsmentioning

confidence: 99%

Virological and Immunological Outcomes of Coinfections

et al. 2018

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“…The use of CE and ME in clinical protein analysis has not shown the expected growth that was expected. There have been some advances in the types of detectors used such as CL , LIF , LED‐LIF , and MS . An interesting study on protein analysis in the vitreous humor of the eye was reported by Reich et al.…”

Section: Applications In Different Clinical Fieldsmentioning

confidence: 99%

Recent advances in CE and microchip‐CE in clinical applications: 2014 to mid‐2017

Phillips

2017

Electrophoresis

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Recent advances in CE and microchip-CE in clinical applications: 2014 to mid-2017CE and microchip CE (ME) are powerful tools for the analysis of a number of different analytes and have been applied to a variety of clinical fields and human samples. This review will present an overview of the most recent applications of these techniques to different areas of clinical medicine during the period of 2014 to mid-2017. CE and ME have been applied to clinical chemistry, drug detection and monitoring, hematology, infectious diseases, oncology, endocrinology, neonatology, nephrology, and genetic screening. Samples examined range from serum, plasma, and urine to lest utilized materials such as tears, cerebral spinal fluid, sweat, saliva, condensed breath, single cells, and biopsy tissue. Examples of clinical applications will be given along with the various detection systems employed.

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“…Although the MS detection technique has a great advantage for the analysis of complex samples, a much higher cost of running the MS instrument limits its high throughput. Chemiluminescence (CL) detection systems, known to have a very sensitive detection nature, have been developed for CE, and have been applied to the analysis of complex matrices such as clinical medicine and food [20,21]. Luminol-oxidant systems are the most frequently used CL reactions, for instance, luminol-H 2 O 2 [22], luminol-H 2 O 2 -horseradish peroxidase [23], luminol-Fe(III) [24], and luminol-H 2 O 2 -Cu(II) [25] have been reported.…”

Section: Introductionmentioning

confidence: 99%

Determination of Sulfonamide Residues in Food by Capillary Zone Electrophoresis with On-Line Chemiluminescence Detection Based on an Ag(III) Complex

Dai

Duan

et al. 2017

IJMS

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The presence of sulfonamide (SA) residues in foods is largely due to the raising of animals with sulfonamide antibiotics added or polluted feedstuff. In this paper, a sensitive method was developed for the determination of the residues of three sulfonamides in animal-derived food; the SAs include sulfadimidine (SDD), sulfadiazine (SDZ), and sulfathiazole (STZ). The method is based on capillary zone electrophoresis (CE) with online chemiluminescence (CL) detection, using an Ag(III) complex as an oxidant. These SAs have an inhibiting effect on the Ag(III)–luminol CL reaction. The electrophoretic buffer is 12.0 mM sodium borate. Under a set of optimized conditions, the linear ranges for the detections were found to be 10.0–200 µg·mL−1 for SDD and SDZ, and 2.0–50.0 µg·mL−1 for STZ. The detection limits were 2.75, 3.14, and 0.65 µg·mL−1 for SDD, SDZ, and STZ, respectively. Relative standard deviations (RSD) for the peak heights were between 2.1% and 2.8% (n = 7). The proposed method was used in the analysis of the SAs in samples from pork meat, chicken meat, and milk, showing satisfactory detection results. A reaction mechanism was also proposed for the Ag(III)–luminol–SA CL reactions. The method has potential applications for the monitoring of residue levels of the three SAs in food, providing food safety data.

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Applications of capillary electrophoresis with chemiluminescence detection in clinical, environmental and food analysis. A review

Cited by 61 publications

References 119 publications

Virological and Immunological Outcomes of Coinfections

Virological and Immunological Outcomes of Coinfections

Recent advances in CE and microchip‐CE in clinical applications: 2014 to mid‐2017

Determination of Sulfonamide Residues in Food by Capillary Zone Electrophoresis with On-Line Chemiluminescence Detection Based on an Ag(III) Complex

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