Applications of capillary electrophoresis on microchip

Dolnı́k, Vladislav; Liu, Shaorong

doi:10.1002/jssc.200500243

Cited by 107 publications

(76 citation statements)

References 139 publications

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“…Understandably, utilizing the potential of microfluidics and lab-on-a-chip led to an early realization of CE-on-a-chip [39]. The small size, low consumption of sample, chemicals and power and a wide variety of applications of the microfluidic chips present a tremendous potential for the development of portable analytical instrumentation [40].…”

Section: Conventional Capillary-based Ce Versus Ce-on-a-chipmentioning

confidence: 99%

“…Chip-based separation technologies have been commercialized by numerous companies (e.g., Agilent, Caliper, Cepheid, Fluidigm, Gyros and Micronics) [40]. Chip-based CE (chip-CE) can be utilized in proteomic analysis [41], DNA separation, including sequencing [42][43][44], fragment sizing and genotyping [45], analysis of low-molecular-weight compounds (e.g., explosive residues and warfare agents) [46], food analysis [47], and analysis of pharmaceuticals, drugs and various analytes in body fluids [48].…”

Section: Conventional Capillary-based Ce Versus Ce-on-a-chipmentioning

confidence: 99%

“…Unlike chip-CE, which has been reviewed numerous times in recent years [40,41,[53][54][55], no review has focused specifically on P-CE instruments using a capillary as the CE-separation format. This appraisal of current status and future development options in P-CE is structured into three sections.…”

Section: Scope Of This Reviewmentioning

confidence: 99%

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Portable capillary-based (non-chip) capillary electrophoresis

Ryvolová

Preisler

Brabazon

et al. 2010

TrAC Trends in Analytical Chemistry

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Miniaturized, portable instrumentation has been gaining popularity in all areas of analytical chemistry. Capillary electrophoresis (CE), due to its main strengths of high separation efficiency, relatively short analysis time and low consumption of chemicals, is a particularly suitable technique for use in portable analytical instrumentation. In line with the general trend in miniaturization in chemistry utilizing microfluidic chips, the main thrust of portable CE (P-CE) systems development is towards chip-based miniaturized CE. Despite this, capillary-based (non-chip) P-CE systems have certain unmatched advantages, especially in the relative simplicity of the regular cylindrical geometry of the CE capillary, maximal volume-to-surface ratio, no need to design and to fabricate a chip, the low costs of capillary compared to chip, and better performance with some detection techniques. This review presents an overview of the state of the art of P-CE and literature relevant to future developments. We pay particular attention to the development and the potential of miniaturization of functional parts for P-CE. These include components related to sample introduction, separation and detection, which are the key elements in P-CE design. The future of P-CE may be in relatively simple, rugged designs (e.g., using a short piece of capillary fixed to a chip-sized platform on which injection and detection parts can be mounted). Electrochemical detection is well suited for miniaturization, so is probably the most suitable detection technique for P-CE, but optical detection is gaining interest, especially due to miniaturized light sources (e.g., light-emitting diodes).

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Section: Conventional Capillary-based Ce Versus Ce-on-a-chipmentioning

confidence: 99%

Section: Conventional Capillary-based Ce Versus Ce-on-a-chipmentioning

confidence: 99%

See 1 more Smart Citation

Portable capillary-based (non-chip) capillary electrophoresis

Ryvolová

Preisler

Brabazon

et al. 2010

TrAC Trends in Analytical Chemistry

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“…The sample is loaded and separated in a 10-to 100-μm bore of 0.1-to 1-m long fused silica capillary or anisotropicaly etched silicon micro-channel (microchip capillary electrophoresis, MCE, reviewed in ref. [231]). The spatial separation of analyte is a subject of analytical performance.…”

Section: High-performance Capillary Electrophoresis (Hpce) Of the No mentioning

confidence: 99%

Bioanalytical profile of the l-arginine/nitric oxide pathway and its evaluation by capillary electrophoresis

Boudko

2007

Journal of Chromatography B

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This review briefly summarizes recent progress in fundamental understanding and analytical profiling of the L-arginine/nitric oxide (NO) pathway. It focuses on key analytical references of NO actions and on the experimental acquisition of these references in vivo, with capillary electrophoresis (CE) and high-performance capillary electrophoresis (HPCE) comprising one of the most flexible and technologically promising analytical platform for comprehensive high-resolution profiling of NO-related metabolites. Second aim of this review is to express demands and bridge efforts of experimental biologists, medical professionals and chemical analysis-oriented scientists who strive to understand evolution and physiological roles of NO and to develop analytical methods for use in biology and medicine.

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“…Microchip capillary electrophoresis (CE) coupled with laser-induced fluorescence (LIF) detection offers CE separations with speed, sensitivity, ease of automation and low consumption of reagents [1][2][3]. Many techniques developed in traditional CE have been transferred onto microchip CE with appropriate modifications [3][4][5][6][7].…”

Section: Introductionmentioning

confidence: 99%

Frontal analysis in microchip CE: A simple and accurate method for determination of protein–DNA dissociation constant

et al. 2007

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Equilibrium constants, such as the dissociation constant (K d ), are a key measurement of noncovalent interactions that are of importance to the proper functioning of molecules in living systems. Frontal analysis (FA) is a simple and accurate capillary electrophoresis (CE) method for the determination of K d . Microchip CE coupled with laser-induced fluorescence (LIF) detection was used to determine K d of protein-DNA interactions using the FA method. A model system of immunoglobulin E (IgE) and the IgE-binding aptamer was selected to demonstrate the capability of FA in microchip CE. Because the fluorescence emission was dependent on the dye migration velocity, the velocity of the free aptamer was adjusted to be the same as that of the aptamer-IgE complex by setting up individual separation voltage configurations for the free and bound aptamers. The ratio of the free and bound aptamers in the equilibrium mixture was directly measured from the heights of their plateaus detected at 1.0 cm from the intersection of the microchip, and no internal standard was needed. The K d of the IgE-aptamer pair was determined as 6 ± 2 nM which is consistent with the reported results (8 nM).

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Applications of capillary electrophoresis on microchip

Cited by 107 publications

References 139 publications

Portable capillary-based (non-chip) capillary electrophoresis

Portable capillary-based (non-chip) capillary electrophoresis

Bioanalytical profile of the l-arginine/nitric oxide pathway and its evaluation by capillary electrophoresis

Frontal analysis in microchip CE: A simple and accurate method for determination of protein–DNA dissociation constant

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