Applications of Anterior Segment Optical Coherence Tomography in Cornea and Ocular Surface Diseases

Han, Sang Beom; Liu, Yu-Chi; Mohamed-Noriega, Karim; Mehta, Jodhbir S.

doi:10.1155/2016/4971572

Cited by 76 publications

(67 citation statements)

References 101 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Anterior LC thickness has been reported in the literature in the range of 8–27 μm using electron microscopy, light microscopy, and microspherules (Danysh, Czymmek, Olurin, Sivak, & Duncan, ). We used OCT as a noncontact, cross‐section imaging technique to perform our measurements ex vivo as it is widely used for both qualitative and quantitative measurements of the anterior segment of the eye (Han, Liu, Noriega, & Mehta, ). Our mean measured thickness of 35 μm is higher compared with the previous reported values.…”

Section: Discussionmentioning

confidence: 99%

Characterizing human decellularized crystalline lens capsules as a scaffold for corneal endothelial tissue engineering

Bogerd

Dhubhghaill

Zakaria

2018

J Tissue Eng Regen Med

View full text Add to dashboard Cite

The idea of transplanting a sheet of laboratory‐grown corneal endothelium dates back to 1978; however, the ideal scaffold is still lacking. We hypothesized that human crystalline lens capsules (LCs) could qualify as a scaffold and aimed to characterize the properties of this material for endothelial tissue engineering. LCs were isolated from donor eyes, stored at −80 °C, and decellularized with water and trypsin‐EDTA. The decellularization was investigated by nuclear staining and counting and the capsule thickness was determined by optical coherence tomography and compared with Descemet's membrane (DM). Transparency was examined by spectrometry, and collagenase degradation was performed to evaluate its resistance to degradation. Cell‐scaffold interaction was assessed by measuring focal adhesions surface area on LC and plastic. Finally, primary corneal endothelial cells were grown on LCs to validate the phenotype. Trypsin‐EDTA decellularized most effectively, removing 99% of cells. The mean LC thickness was 35.76 ± 0.43 μm, whereas DM measured 25.93 ± 0.26 μm (p < .0001). Light transmission was 90% for both LC and DM. On a collagenase challenge, LC and amniotic membrane were digested after 13 hr, whereas DM was digested after 17 hr. The surface area of focal adhesions for cells grown on coated LCs was at least double that compared with other conditions, whereas tight junctions, ion pumps, and hexagonal morphology were well maintained when endothelial cells were cultured on LCs. In conclusion, LCs demonstrate excellent scaffolding properties for tissue engineering and sustain the cell phenotype and can be considered a suitable substrate for ocular tissue engineering or as a template for future scaffolds.

show abstract

Section: Discussionmentioning

confidence: 99%

Characterizing human decellularized crystalline lens capsules as a scaffold for corneal endothelial tissue engineering

Bogerd

Dhubhghaill

Zakaria

2018

J Tissue Eng Regen Med

View full text Add to dashboard Cite

show abstract

“…Evaluation of corneal surgical interventions with SS-OCT covers a large variety of techniques, such as refractive surgery, keratoplasty, intrastromal corneal rings and others ( Fig. 13.10) [1,11,12]. In general, SS-OCT measurements have been shown to have better repeatability of corneal biometrical measurements compared with Scheimpflug, for corneal graft evaluation [8].…”

Section: Ss-oct and Cornea Evaluationmentioning

confidence: 99%

Anterior Segment OCT

Asam

Polzer

Tafreshi

et al. 2019

High Resolution Imaging in Microscopy and Ophthalmology

View full text Add to dashboard Cite

“…AS‐OCT can be categorised into time‐domain and frequency‐domain OCT. In time‐domain OCT, a moving reference mirror is used to obtain serial information about tissue reflectivity to infrared light, which in turn corresponds to tissue depth . Therefore, time‐domain OCT is limited by the time taken for the reference mirror to scan the tissue plane .…”

mentioning

confidence: 99%

“…Therefore, time‐domain OCT is limited by the time taken for the reference mirror to scan the tissue plane . Time‐domain OCT systems include the Visante OCT (Carl Zeiss Meditec, Oberkochen, Germany) and slitlamp OCT (Heidelberg Engineering GmbH, Heidelberg, Germany) . The Visante OCT has a wavelength of 1,310 nm, a 16 mm scan width, and 6 mm scan depth .…”

mentioning

confidence: 99%

Anterior segment optical coherence tomography and its clinical applications

Wang

Cornish

Grigg

et al. 2019

Clinical and Experimental Optometry

View full text Add to dashboard Cite

Anterior segment optical coherence tomography (AS-OCT) has become one of the cornerstones of non-contact imaging modalities for assessing such structures as the cornea, anterior chamber angle, aqueous outflow pathway, sclera, and ocular surface structures. As such, it has a broad range of clinical applications, which have been independently reported in the literature. This paper aims to present a review of extant literature on the utility of AS-OCT and its efficacy in clinical applications, and to evaluate the quality of available evidence. The following databases were searched from inception to 24 June 2018: Medline via Ovid, Cochrane Central Register of Controlled Trials, PubMed, World Health Organization International Clinical Trials Registry Platform, EMBASE, and CINAHL. Bibliographies of identified papers were hand searched. Inclusion criteria: articles describing or assessing the use of OCT for visualising the AS. The authors excluded studies without an identified primary outcome variable. One author independently selected studies, extracted data, and assessed for risk of bias using PRISMA guidelines. This review included 82 studies, of which there were 11 cohort studies, 37 case series, 10 case studies, 21 comparative observational studies, and three non-systematic review articles. Primary outcome variables included anterior chamber angle, angle opening distance, angle recess area, trabecular iris angle, trabeculairis space area, corneal thickness, tear meniscus height, tear meniscus area, tear meniscus volume, and the morphology of AS structures, including the ocular surface, blebs, flaps, and graft sites. This review attempts to encompass the breadth and depth of evidence for AS-OCT in the arena of diagnostics, therapeutics, and prognostics. At the same time, it brings to light the dearth of high-level evidence on this topic, suggesting the important role of randomised controlled trials and meta-analyses for the future validation of this technology.

show abstract

Applications of Anterior Segment Optical Coherence Tomography in Cornea and Ocular Surface Diseases

Cited by 76 publications

References 101 publications

Characterizing human decellularized crystalline lens capsules as a scaffold for corneal endothelial tissue engineering

Characterizing human decellularized crystalline lens capsules as a scaffold for corneal endothelial tissue engineering

Anterior Segment OCT

Anterior segment optical coherence tomography and its clinical applications

Contact Info

Product

Resources

About