Applications of a capillary based X-ray microfluorescence system

Bernasconi, Gabriel D.; Haselberger, N.; Markowicz, A.; Valković, V.

doi:10.1016/0168-583x(94)95298-1

Cited by 11 publications

(6 citation statements)

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“…3 In order to follow the trends in the XRF field, the laboratory at Seibersdorf contributed to the development of portable XRF spectrometers and relevant methodologies for in situ applications 4 as well as a capillary-based x-ray microfluorescence system for microanalysis and microtomography. 5,6 To increase the direct benefits for laboratories in member states, the XRF laboratory at Seibersdorf provided analytical services (mostly for cross-check analysis) and organised worldwide proficiency tests (PTs) for biological materials and air particulates collected on filters-the PTs are now organised systematically, often in support of the laboratories involved in regional TC projects in the Asia and Pacific Region, Europe and Latin America.…”

Section: Short History -Why An Xrf Laboratory At Iaea?mentioning

confidence: 99%

Activities in the IAEA X‐ray fluorescence laboratory at Seibersdorf

et al. 2006

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This paper describes the specific philosophy behind the functioning of the IAEA XRF Laboratory at Seibersdorf and its role in the XRF community. Some examples of the research and development activities and the results obtained are given. They include methodological development and construction of XRF instruments in order to extend the applicability range of the XRF technique, particularly in support of applications of the analytical technique in developing IAEA member states. The contribution of the laboratory to the training in methodology and applications of XRF techniques and to development of QA/QC procedures is also emphasised.

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Section: Short History -Why An Xrf Laboratory At Iaea?mentioning

confidence: 99%

Activities in the IAEA X‐ray fluorescence laboratory at Seibersdorf

et al. 2006

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“…X‐ray microfluorescence (μXRF) technique is a method, based on localized excitation of a microscopically small (on the order of μm) area of the sample that allows the investigation of changes in the elemental composition. This is particularly important in view of the fact that normal and pathological biochemical processes occur at the micro and submicron range . Synchrotron radiation (SR) generates quite strong X‐rays (and other electromagnetic waves, such as ultra violet, visible, and infrared light).…”

Section: Introductionmentioning

confidence: 99%

Zinc distribution in human prostate carcinoma cell line using synchrotron X‐ray microfluorescence

et al. 2017

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a Cancer is a worldwide public health problem, and its incidence in the world grew by 20% in the last decade. Zinc, an essential trace element, is involved in many cellular processes. Concerning prostate cancer, Zn could inhibit the growth of tumor cells, by inducing cell cycle arrest or apoptosis. X-ray microfluorescence (μXRF) is an elemental analysis technique that allows mapping biologically important elements at a submillimeter scale, with high sensitivity and negligible damage to the sample. In this study, we investigated cell viability through colorimetric cytotoxicity assay (MTT) and the behavior of human prostate cell lines obtained from normal (RWPE-1) and tumorigenic (DU145) epithelium, in three-dimensional spheroid cultures after supplementation with ZnCl 2 for 24 and 48 hr using synchrotron μXRF. The measurements were performed at the Synchrotron Light National Laboratory (Campinas, Brazil). The results of μXRF showed that Zn intensity decreased in the DU145 tumor cells independent of supplementation or treatment time, and in normal cells, the intensity increased with supplementation and treatment time. The MTT assay results showed no significant differences, which indicates that the cell viability did not change. Our results indicate that μXRF can be used as an important tool to understand the mechanism of the loss of ability to capture zinc by prostate cancer cells.

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“…The system further consists of a Si(Li) detect or, with a resolution of 170 eV for the 5.90 keV line, coupled to a com puter controlled ADC -card. Further details about the system have been given elsewhere [11,13,14].…”

Section: Methodsmentioning

confidence: 99%