Application timing of nitric oxide ameliorates on deleterious effects of salinity on growth and fruit quality of strawberry cv. ‘Selva’

Abstract

Abstract. BACKGROUND:The exogenous application of nitric oxide (NO) has proved to protect plant against deleterious impact of salinity. OBJECTIVE: The probable influence of application timing has been neglected in most studies. In order to evaluate the influence of application timing of NO on 'Selva' strawberry plants the following experiments were carried out. METHOD: After establishment of 'Selva' daughter plants they were sprayed using 50 or 75 M NO solution (Sodium nitroprusside was used as NO donor), one week before, simultaneously and one week after start of an established 40 mM NaCl salinity stress. RESULTS: Indicated that saline conditions had adverse influence on plants and that NO at both concentrations ameliorated these salinity effects as plants treated with 50 or 75 M of NO had larger shoot and root dry weight, higher number of inflorescences and quality parameters such as TSS in comparison to salinity stress exposed plants. Time of application was important, and plants treated with NO one week before beginning of salinity stress had highest ameliorative effect. CONCLUSION: It seems that application of NO prior the stress increases the stress avoidance strategy of plants.

Comparison of fruit quality characteristics and polyphenolic compounds in seven Iranian pomegranate cultivars

“…The pigment content was calculated as cyaniding-3-glucosid, where MW=449.2 and ε=26.900. 25 The total polyphenols concentration of fruits was measured by the Folin-Ciocalteau reagent using gallic acid as standard. The juice (1ml) was mixed with 5 ml Folin-Ciocalteau reagent (previously diluted 10-fold with distilled water) and 4ml sodium bicarbonate (7.5%w/v), and the mixture was diluted to 100 ml with distilled water.…”

“…Total polyphenolics content was expressed as gallic acid equivalents (the concentration of gallic acid was established from a calibration curve) in mg/100 ml juice (mg GAE.100 ml -1 juice). 25 Ascorbic acid concentration in fruits was measured spectrophotometrically. To 100µl of fruit juice 10 ml of methaphosphoric acid (1%) was added, then to 1 ml of this mixture 9 ml of indophenol (50µM) was added and vortexed and read with spectrometer at 515 nm.…”

“…The results were expressed as mg.100 -1 ml juice. 25 Antioxidant activity was assessed according to the method of Brand-Williams et al 26 Briefly, 100µl of pomegranate juice diluted in the ratio of 1:100 with methanol:water (6:4 v/v) was mixed with 2 ml of 0.1 mM DPPH in methanol. The mixtures were shaken vigorously and left to stand for 30 min.…”