Application potential of biogenically synthesized silver nanoparticles using Lythrum salicaria L. extracts as pharmaceuticals and catalysts for organic pollutant degradation
Abstract:This study was designed to evaluate the optimal conditions for the eco-friendly synthesis of silver nanoparticles (AgNPs) using Lythrum salicaria L. (Lythraceae) aqueous extracts and their potential application and safe use.
“…The presence of a single SPR peak could explain the spherical shape of AgNPs [ 32 ]. The obtained results were in accordance with other studies focusing on AgNPs synthesized using L. salicaria extracts, in which the SPR band was observed at 415 nm [ 16 ] or in the 396–415 nm range [ 17 ]. The calculated concentration of AgNPs in the colloidal solution was found to be 2 × 10 −10 mol/L.…”
Section: Resultssupporting
confidence: 92%
“…To the best of our knowledge, the present study is the only one that has used the Taguchi model to establish the reaction conditions for AgNPs synthesized using an L. salicaria extract. Moreover, the traditional optimization of synthesis can be found in a single study carried out by Srećković et al, which led to the following conditions: 20 mM AgNO 3 concentration, 25 ℃, pH 12, and 30 min reaction time [ 17 ]. In both cases, the synthesis was optimal at alkaline pH, which can be explained by the change in dissociation constants values for functional groups involved in the reduction process, which leads to an increase in the availability of compounds for the synthesis process [ 28 ].…”
Section: Resultsmentioning
confidence: 99%
“…Successful biological AgNPs synthesis using L. salicaria extract has recently been reported in the literature, although the studies were conducted in order to incorporate the nanoparticles in nanohybrids, with cellulose, chitosan and lignocellulose [ 16 ]. Other comparative research was conducted on AgNPs synthesized using aerial parts and root extracts [ 17 ]. Thus, the novelty of the present study is associated with AgNPs synthesis via a L. salicaria aqueous extract, establishing optimal reaction conditions, for the first time, using the Taguchi design.…”
This research describes an eco-friendly green route for the synthesis of AgNPs using an aqueous extract of Lythrum salicaria. Taguchi design was used to optimize the synthesis method, taking into account various working conditions. The optimum parameters were established using a 3 mM AgNO3 concentration, a 1:9 extract:AgNO3 volume ratio, a pH value of 8, 60 ℃ temperature, and 180 min reaction time. The synthesized AgNPs were characterized using UV-Vis and FTIR spectroscopy, and TEM and EDX analysis. The SPR band at 410 nm, as well as the functional groups of biomolecules identified by FTIR and the EDX signals at ~3 keV, confirmed the synthesis of spherical AgNPs. The average AgNPs size was determined to be 40 nm, through TEM, and the zeta potential was −19.62 mV. The antimicrobial assay showed inhibition against S. aureus and C. albicans. Moreover, the results regarding the inhibition of lipoxygenase and of peroxyl radical-mediated hemolysis assays were promising and justify further antioxidant studies.
“…The presence of a single SPR peak could explain the spherical shape of AgNPs [ 32 ]. The obtained results were in accordance with other studies focusing on AgNPs synthesized using L. salicaria extracts, in which the SPR band was observed at 415 nm [ 16 ] or in the 396–415 nm range [ 17 ]. The calculated concentration of AgNPs in the colloidal solution was found to be 2 × 10 −10 mol/L.…”
Section: Resultssupporting
confidence: 92%
“…To the best of our knowledge, the present study is the only one that has used the Taguchi model to establish the reaction conditions for AgNPs synthesized using an L. salicaria extract. Moreover, the traditional optimization of synthesis can be found in a single study carried out by Srećković et al, which led to the following conditions: 20 mM AgNO 3 concentration, 25 ℃, pH 12, and 30 min reaction time [ 17 ]. In both cases, the synthesis was optimal at alkaline pH, which can be explained by the change in dissociation constants values for functional groups involved in the reduction process, which leads to an increase in the availability of compounds for the synthesis process [ 28 ].…”
Section: Resultsmentioning
confidence: 99%
“…Successful biological AgNPs synthesis using L. salicaria extract has recently been reported in the literature, although the studies were conducted in order to incorporate the nanoparticles in nanohybrids, with cellulose, chitosan and lignocellulose [ 16 ]. Other comparative research was conducted on AgNPs synthesized using aerial parts and root extracts [ 17 ]. Thus, the novelty of the present study is associated with AgNPs synthesis via a L. salicaria aqueous extract, establishing optimal reaction conditions, for the first time, using the Taguchi design.…”
This research describes an eco-friendly green route for the synthesis of AgNPs using an aqueous extract of Lythrum salicaria. Taguchi design was used to optimize the synthesis method, taking into account various working conditions. The optimum parameters were established using a 3 mM AgNO3 concentration, a 1:9 extract:AgNO3 volume ratio, a pH value of 8, 60 ℃ temperature, and 180 min reaction time. The synthesized AgNPs were characterized using UV-Vis and FTIR spectroscopy, and TEM and EDX analysis. The SPR band at 410 nm, as well as the functional groups of biomolecules identified by FTIR and the EDX signals at ~3 keV, confirmed the synthesis of spherical AgNPs. The average AgNPs size was determined to be 40 nm, through TEM, and the zeta potential was −19.62 mV. The antimicrobial assay showed inhibition against S. aureus and C. albicans. Moreover, the results regarding the inhibition of lipoxygenase and of peroxyl radical-mediated hemolysis assays were promising and justify further antioxidant studies.
“… 30 Because of their environmentally-friendly nature, biosynthetic processes are being researched for the synthesis of Ag NPs. 37 In biosynthesis methods, the reaction time for the reduction of silver ions requires more time due to the slow rate of the reaction. For example, the synthesis of Ag NPs by bacteria requires 24–120 h to complete the reduction process, with high polydispersity and aggregation of the NPs.…”
Combining Ocimum sanctum and Trigonella foenum-graecum L leaf water extracts synergistically act as a reducing and capping agent for the synthesis of narrow polydisperse silver nanoparticles with controlled sizes depending on the precursor (AgNO3) concentration.
The hemolytic activity assay is a versatile tool for fast primary toxicity studies. This work presents a systematic study of the hemolytic properties of ArgovitTM silver nanoparticles (AgNPs) extensively studied for biomedical applications. The results revealed an unusual and unexpected bell-shaped hemolysis curve for human healthy and diabetic donor erythrocytes. With the decrease of pH from 7.4 and 6.8 to 5.6, the hemolysis profiles for AgNPs and AgNO3 changed dramatically. For AgNPs, the bell shape changed to a step shape with a subsequent sharp increase, and for AgNO3 it changed to a gradual increase. Explanations of these changes based on the aggregation of AgNPs due to the increase of proton concentration were suggested. Hemolysis of diabetic donor erythrocytes was slightly higher than that of healthy donor erythrocytes. The meta-analysis revealed that for only one AgNPs formulation (out of 48), a bell-shaped hemolysis profile was reported, but not discussed. This scarcity of data was explained by the dominant goal of studies consisting in achieving clinically significant hemolysis of 5–10%. Considering that hemolysis profiles may be bell-shaped, it is recommended to avoid extrapolations and to perform measurements in a wide concentration interval in hemolysis assays.
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