Green Synthesis and Characterization of Silver Nanoparticles Using a Lythrum salicaria Extract and In Vitro Exploration of Their Biological Activities

Abstract

This research describes an eco-friendly green route for the synthesis of AgNPs using an aqueous extract of Lythrum salicaria. Taguchi design was used to optimize the synthesis method, taking into account various working conditions. The optimum parameters were established using a 3 mM AgNO3 concentration, a 1:9 extract:AgNO3 volume ratio, a pH value of 8, 60 ℃ temperature, and 180 min reaction time. The synthesized AgNPs were characterized using UV-Vis and FTIR spectroscopy, and TEM and EDX analysis. The SPR band at 410 nm, as well as the functional groups of biomolecules identified by FTIR and the EDX signals at ~3 keV, confirmed the synthesis of spherical AgNPs. The average AgNPs size was determined to be 40 nm, through TEM, and the zeta potential was −19.62 mV. The antimicrobial assay showed inhibition against S. aureus and C. albicans. Moreover, the results regarding the inhibition of lipoxygenase and of peroxyl radical-mediated hemolysis assays were promising and justify further antioxidant studies.

Green Synthesis of Silver Nanoparticles Using Salvia verticillata and Filipendula ulmaria Extracts: Optimization of Synthesis, Biological Activities, and Catalytic Properties

“…The bands at 1404 and 1448 cm −1 in SVE and FUE spectra, may be assigned to C-O bending vibrations of carboxylic acids or esters, O-H bending vibration of alcohol and phenols, and bending vibration of N-H in amides. The medium broad bands at 1265 and 1073 cm −1 are found in the SVE specter and in FUE at 1234 and 1035 cm −1 ; this is also the C–O stretching mode of the alcohols, carboxylic acids, esters, ethers, carbohydrates residues, and flavonoids (C-O-C group) [ 28 , 32 , 33 , 34 ].…”

“…This transfer reduces activating energy for CR reduction and stabilizes the system [ 53 ]. AgNPs also represent a suitable surface for attaching both reactants, CR and BH 4 ‾ , and allowing the reaction to take place [ 34 ]. Fauda et al [ 58 ] have shown that light can also initiate silver nanoparticle catalysis.…”