Application of water-soluble nanofilters for collection of airborne Mycobacterium tuberculosis DNA in hospital wards

Vladimirsky, Mikhail; Shipina, L K; Makeeva, E.S.; Alyapkina, Yu. S.; Mikheev, Andrei Y.; Морозов, В. Н.

doi:10.1016/j.jhin.2016.02.009

Cited by 5 publications

(1 citation statement)

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“…Pressure-driven flow of a sample through a membrane makes it possible to collect the analyte from a relatively large volume and thereby improve the analytical characteristics of the biosensor. Several studies demonstrate the implementation of this approach to collect an analyte from the air using a vacuum cleaner [8,9]. For liquid samples, cyclic pumping through the membrane for gradual depletion of the analyte from the solution also looks promising [10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Pressure-Driven Sample Flow through an Electrospun Membrane Increases the Analyte Adsorption

Maslakova

Prusakov

Sidorova

et al. 2023

Micro

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Electrospun polymer membranes are regarded as prospective biosensor components due to their large specific surface area and diverse opportunities for chemical modifications. However, their intricate porous structure can impede diffusion and render some analyte-binding sites inaccessible. To overcome these diffusion limitations and improve analyte adsorption onto the polymer, a pressure-driven sample flow through the membrane can be employed. To date, the efficiency of pressure-driven analyte delivery into these membranes has not been quantified. Here, we compare forced flow and passive sample diffusion through poly(dioxanone) electrospun membranes. We examine two model analytes, BSA and interleukin-1 beta (IL1b), to address both non-specific and specific binding. Following exposure of the membranes to the test solutions, we measured the residual concentrations of the analytes using fluorometry and enzyme-linked immunosorbent assay (ELISA) techniques. The pressure-driven sample loading was superior to passive diffusion, with a 2.8–11.5-fold change for physical adsorption and a 2.4–3.4-fold difference for specific binding. Our data can be useful for the development of immunoassays and microfluidic devices.

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