A fieldable electrostatic air sampler enabling tuberculosis detection in bioaerosols

Sousa, Nuno Rufino de; Sandström, Niklas; Shen, Lei; Håkansson, Kathleen; Vezozzo, Rafaella; Udekwu, Klas I.; Croda, Júlio; Rothfuchs, Antonio Gigliotti

doi:10.1016/j.tube.2019.101896

Cited by 17 publications

(21 citation statements)

References 38 publications

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“…To investigate this, we decided to swab various surfaces of the Coriolis after air sampling to see if any parts other than the collector cone became positive after collection. We chose to aerosolize Bg spores, the Anthrax simulant, since we have successfully extracted Bg by surface swabs in the past ( Rufino de Sousa et al , 2020 ). Thus, we aerosolized Bg spores in the chamber and used the Coriolis to collect Bg bioaerosols.…”

Section: Resultsmentioning

confidence: 99%

“…Contained aerosol dispersal experiments were performed inside a large, airtight, flexible polyvinyl chloride enclosure mounted on a metal–support frame (Solo Containment, UK). The enclosure measures 270 cm ( L ) × 165 cm ( W ) × 255 cm ( H ) with an inner volume of 9.3 m 3 ( Rufino de Sousa et al , 2020 ). It was assembled and kept inside a Biosafety Level (BSL)-2 laboratory.…”

Section: Methodsmentioning

confidence: 99%

“…In the above studies the Coriolis was used mainly in conjunction with culture and nucleic acid amplification methods, although microscopy and in some cases spoligotyping and sequencing were also performed. In addition, flow cytometry, solid-phase cytometry, and immunoassays have been employed to detect aerosolized microbes or their simulants collected on the Coriolis ( Langer et al , 2012 ; Le Goff et al , 2012 ; Rufino de Sousa et al , 2020 ). The Coriolis has also been used to detect airborne traces of the explosive trinitrotoluene through an electrochemical assay ( Caygill et al , 2013 ).…”

Section: Introductionmentioning

confidence: 99%

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Operative and Technical Modifications to the Coriolis® µ Air Sampler That Improve Sample Recovery and Biosafety During Microbiological Air Sampling

Sousa

Shen

Silcott³

et al. 2020

Annals of Work Exposures and Health

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Detecting infectious aerosols is central for gauging and countering airborne threats. In this regard, the Coriolis® µ cyclonic air sampler is a practical, commercial collector that can be used with various analysis methods to monitor pathogens in air. However, information on how to operate this unit under optimal sampling and biosafety conditions is limited. We investigated Coriolis performance in aerosol dispersal experiments with polystyrene microspheres and Bacillus globigii spores. We report inconsistent sample recovery from the collector cone due to loss of material when sampling continuously for more than 30 min. Introducing a new collector cone every 10 min improved this shortcoming. Moreover, we found that several surfaces on the device become contaminated during sampling. Adapting a high efficiency particulate air-filter system to the Coriolis prevented contamination without altering collection efficiency or tactical deployment. A Coriolis modified with these operative and technical improvements was used to collect aerosols carrying microspheres released inside a Biosafety Level-3 laboratory during simulations of microbiological spills and aerosol dispersals. In summary, we provide operative and technical solutions to the Coriolis that optimize microbiological air sampling and improve biosafety.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Operative and Technical Modifications to the Coriolis® µ Air Sampler That Improve Sample Recovery and Biosafety During Microbiological Air Sampling

Sousa

Shen

Silcott³

et al. 2020

Annals of Work Exposures and Health

Self Cite

View full text Add to dashboard Cite

show abstract

“…To investigate this, we decided to swab various surfaces of the Coriolis after air sampling to see if any parts other than the collector cone became positive after collection. We chose to aerosolize Bacillus globigii ( Bg ) spores, the Anthrax simulant, since we have successfully extracted Bg by surface swabs in the past (Rufino de Sousa et al, 2020). Thus, we aerosolized Bg spores in the chamber and used the Coriolis to collect Bg bioaerosols.…”

Section: Resultsmentioning

confidence: 99%

“…Introduction of a cumulative sampling protocol and a HEPA-filter adaptation to the device improved these shortcomings. We have recently used the HEPA-modified Coriolis with the cumulative sampling protocol in our aerosol chamber to investigate the performance of a portable electrostatic air sampler for tuberculosis (Rufino de Sousa et al, 2020). A similar HEPA-modified Coriolis has also been used in a clinical, experimental setting to quantify M. tuberculosis from human bioaerosols (Patterson et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Operative and technical modifications to the Coriolis^®µ air sampler that improve sample recovery and biosafety during microbiological air sampling

Sousa

Shen

Silcott

et al. 2020

Preprint

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23Detecting infectious aerosols is central for gauging and countering airborne threats. In 24 this regard the Coriolis ® µ cyclonic air sampler is a practical, commercial collector that can be 25 used with various analysis methods to monitor pathogens in air. However, information on how 26 to operate this unit under optimal sampling and biosafety conditions is limited. We investigated 27 Coriolis performance in aerosol dispersal experiments with polystyrene microspheres and 28 Bacillus globigii spores. We report inconsistent sample recovery from the collector cone due 29 to loss of material when sampling continuously for more than 30 min. Introducing a new 30 collector cone every 10 min improved this shortcoming. Moreover, we found that several 31 surfaces on the device become contaminated during sampling. Adapting a HEPA-filter system 32 to the Coriolis prevented contamination without altering collection efficiency or tactical 33 deployment. A Coriolis modified with these operative and technical improvements was used 34 to collect aerosols carrying microspheres released inside a Biosafety Level-3 laboratory during 35 simulations of microbiological spills and aerosol dispersals. In summary, we provide operative 36 and technical solutions to the Coriolis that optimize microbiological air sampling and improve 37 biosafety. 38 39 Abstract word count: 180 40 41 42

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Micro and Nanoscale Technologies for Diagnosis of Viral Infections

Nasrollahi

Haghniaz

Hosseini

et al. 2021

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Viral infection is one of the leading causes of mortality worldwide. The growth of globalization significantly increases the risk of virus spreading, making it a global threat to future public health. In particular, the ongoing coronavirus disease 2019 (COVID‐19) pandemic outbreak emphasizes the importance of devices and methods for rapid, sensitive, and cost‐effective diagnosis of viral infections in the early stages by which their quick and global spread can be controlled. Micro and nanoscale technologies have attracted tremendous attention in recent years for a variety of medical and biological applications, especially in developing diagnostic platforms for rapid and accurate detection of viral diseases. This review addresses advances of microneedles, microchip‐based integrated platforms, and nano‐ and microparticles for sampling, sample processing, enrichment, amplification, and detection of viral particles and antigens related to the diagnosis of viral diseases. Additionally, methods for the fabrication of microchip‐based devices and commercially used devices are described. Finally, challenges and prospects on the development of micro and nanotechnologies for the early diagnosis of viral diseases are highlighted.

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A fieldable electrostatic air sampler enabling tuberculosis detection in bioaerosols

Cited by 17 publications

References 38 publications

Operative and Technical Modifications to the Coriolis® µ Air Sampler That Improve Sample Recovery and Biosafety During Microbiological Air Sampling

Operative and Technical Modifications to the Coriolis® µ Air Sampler That Improve Sample Recovery and Biosafety During Microbiological Air Sampling

Operative and technical modifications to the Coriolis^®µ air sampler that improve sample recovery and biosafety during microbiological air sampling

Micro and Nanoscale Technologies for Diagnosis of Viral Infections

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A fieldable electrostatic air sampler enabling tuberculosis detection in bioaerosols

Cited by 17 publications

References 38 publications

Operative and Technical Modifications to the Coriolis® µ Air Sampler That Improve Sample Recovery and Biosafety During Microbiological Air Sampling

Operative and Technical Modifications to the Coriolis® µ Air Sampler That Improve Sample Recovery and Biosafety During Microbiological Air Sampling

Operative and technical modifications to the Coriolis®µ air sampler that improve sample recovery and biosafety during microbiological air sampling

Micro and Nanoscale Technologies for Diagnosis of Viral Infections

Contact Info

Product

Resources

About

Operative and technical modifications to the Coriolis^®µ air sampler that improve sample recovery and biosafety during microbiological air sampling