Application of ultrasound contra infection with secondary hydatidosis in mice

Ramdan, Baraa B.; Ali, Asmaa A.

doi:10.33899/ijvs.2019.126132.1240

Cited by 5 publications

(5 citation statements)

References 21 publications

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“…As regards animal sex, the female has an infection rate of 9.69% compared to males at 7.09%, and the primary infected muscle is the esophagus, found in 76.3%. Then the infection rate in both tracheal muscles and tongue is 35.3% and 33.8%, respectively, and finally, the diaphragm muscles are involved in 18.71% due to the abundance of stray dogs and cats (40,41).…”

Section: Discussionmentioning

confidence: 99%

The prevalence of parasitic infections among slaughtered animals in mechanical abattoir

Felefel¹,

Shabian²,

Eassa³

et al. 2023

IJVS

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Gastrointestinal parasites, such as helminths and protozoa, are abundant parasitic agents of livestock, particularly ruminants. The current study aims to determine the prevalence rate of parasitic infections in large ruminate animals slaughtered in the mechanical abattoir in Alexandria governorate, Egypt. The prevalence was identified through the records of parasitic infections detected in the fecal matter of large ruminate animals in addition to the recorded postmortem examination findings. Three hundred sixty-four slaughtered large ruminant animals were selected, labeled, and subjected to fecal sampling and postmortem examination. Each fecal sample was tested by three different techniques; the formalin-ether concentration, Ziehl-Neelsen hot-stained, and saturated saline flotation to diagnose all parasite types. One hundred thirty positive cases (35.71%) were determined during the fecal sample examination, and 76 cases (20.87%) were found in postmortem examination. Formalin-ether processing of fecal samples yielded the highest number of pathogens; Entamoeba species were detected in 98 fecal samples (26.69%), and Fasciola species eggs were detected in 14 samples (3.84%). The postmortem examination revealed Fasciola hepatica and F. gigantica in 13 animals (3.57%), mixed lung and liver hydatid cysts in 32 animals (8.79%), whereas liver hydatid cysts only were identified in 15 animals (4.12%). Finally, Sarcocystosis infection was detected in 3.57%. In conclusion, the parasitic infections of large ruminant animals are a leading cause of pathogenicity in large animals, leading to economic losses. Prevention and control measures must be implemented by mechanical abattoirs according to the hazard analysis critical control point system.

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Section: Discussionmentioning

confidence: 99%

The prevalence of parasitic infections among slaughtered animals in mechanical abattoir

Felefel¹,

Shabian²,

Eassa³

et al. 2023

IJVS

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“…Many techniques were used against infestation with hydatid disease in experiential animals, such as irradiation with laser, direct electrical current, probiotics and ultrasound (31)(32)(33)(34)(35)(36)(37), all techniques revealed considerable influence to the disease. All the techniques exhibited valuable efficiency against protoscoleces in vitro and cystic echinococcosis in mice.…”

Section: Discussionmentioning

confidence: 99%

Effect of selenium nanoparticles against protoscoleces of Echinococcus granulosus in vitro and hydatid cysts in mice

Mohammed¹,

Ali²

2022

IJVS

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This study determined the influence of selenium nanoparticles on the vitality of the protoscoleces of Echinococcus granulosus in vitro; seven concentrations were used: 50, 100, 150, 200, 250, 350, and 500 µg/ml for different exposure times: ten, twenty, thirty, and sixty minutes, respectively. Albino mice Mus musculus were injected with protoscoleces exposed to nanoparticles at 100, 150, and 200 µg/ml concentrations for 60 minutes. In contrast, control groups were injected with non-exposed protoscoleces. Mice were dissected three, four-, and five-months post infestation. Many criteria were relied on: numbers, weights, diameters of growing hydatid cysts, and their reduction proportion. The outcomes uncovered an apparent influence of selenium nanoparticles on the viability of protoscoleces of Echinococcus granlosus by the increase in exposure time in vitro, as well as diminish in the numbers of the larvae in processed mice versus the unprocessed collection; no cysts evolved inside processed mice at the concentration 200 µg/ml, threeand four-months post-infection. In contrast, the reduction rate was 90% in mice injected with exposed protoscoleces at the concentration of 150 µg/ml next 4 and 5 months of infection.

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“…Apply 20 ul of stain on a slide using a light microscope and examine it. Because protoscolices membranes excluded eosin, bright green protoscolices were regarded as living, whereas red protoscolices were judged dead due to pigment acceptance (17).…”

Section: Assessment Of E Granulosus Protoscolices Viabilitymentioning

confidence: 99%

Secondary bacterial infection of hydatid cysts infected livestock animals (In vitro study)

Yahya

Mohi-Aldeen

2022

IJVS

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Isolation and identification of the bacteria-infected hydatid cysts of livestock animals were carried out. The study lasted for five months. A total of 302 slaughtered livestock were checked to find hydatid cyst naturally infected livers and lungs of sheep and goat at local Mosul abattoir/ Nineveh/ Iraq. Each cysts' aspirated fluid was cultured separately on blood or nutrient agar at 37C for 24 hours then detected secondary bacteria. Each bacterium was incubated independently with 1000 viable protoscolices in a culture tube containing tryptic soy broth at 37°C and examined every two hours for 6 hours, followed by 12, 24, 36, and 48 hours afterward. The degeneration rate of protoscolices resulting from treatment with bacteria isolated depending on viability was studied in vitro. The results were revealed that the liver was the preponderant affected organ in sheep and goats. About 57.1% of sheep and 50% of goats harbored fertile cysts. Escherichia coli, Klebsiella pneumonia, Staphylococcus aureus, Proteus mirabilis, and Pseudomonas aeruginosa bacteria were observed in the infected cysts. The most common bacteria infect the cyst was Escherichia coli. The protoscoleces treated with the bacterial isolates had completely deteriorated, whereas 97% of protoscoleces throughout the control groups were still alive and intact even after completing the incubation time. The experiments detect a significant time-dependent scolicidal effect on decreased viability of protoscolices in vitro study. This could pave the door for more research into the scolicidal validity of bacteria or their by-products as protoscolices both in vitro and in vivo.

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Application of ultrasound contra infection with secondary hydatidosis in mice

Cited by 5 publications

References 21 publications

The prevalence of parasitic infections among slaughtered animals in mechanical abattoir

The prevalence of parasitic infections among slaughtered animals in mechanical abattoir

Effect of selenium nanoparticles against protoscoleces of Echinococcus granulosus in vitro and hydatid cysts in mice

Secondary bacterial infection of hydatid cysts infected livestock animals (In vitro study)

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