Application of tyramide signal amplification system to immunohistochemistry: A potent method to localize antigens that are not detectable by ordinary method

Toda, Yoshinobu; Kono, Katsuhiko; Abiru, Hitoshi; Kokuryo, Kumiko; Endo, Mareyuki; Yaegashi, Hiroshi; Fukumoto, Manabu

doi:10.1046/j.1440-1827.1999.00875.x

Cited by 117 publications

(87 citation statements)

References 8 publications

(9 reference statements)

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“…Immunohistochemistry was carried out as described. 16 To semi-quantitate the immunostaining results, the slides were scored independently by two evaluators (J.K. and H.H.) for AID staining.…”

Section: Methodsmentioning

confidence: 99%

Activation-induced cytidine deaminase links bile duct inflammation to human cholangiocarcinoma

et al. 2008

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Chronic inflammation plays a critical role in oncogenesis in various human organs. Epidemiological studies have demonstrated that patients with primary sclerosing cholangitis have a predisposition to develop cholangiocarcinoma (CC). However, the molecular mechanisms that account for the development of bile duct carcinomas are not well defined. We recently provided evidence that activation-induced cytidine deaminase (AID), a member of the DNA/ RNA editing enzyme family, is implicated in human tumorigenesis via its mutagenic activity. We found here that ectopic AID production is induced in response to tumor necrosis factor-␣ ( C holangiocarcinoma (CC) is an epithelial neoplasm that originates from the bile duct and can occur at any level of the biliary tree. 1,2 The incidence CC is increasing worldwide; it is the second most common primary hepatobiliary malignancy. 2 Although most CC arise in the absence of apparent risk factors, chronic inflammation of the biliary epithelium plays a critical role for their development. 2 In fact, primary sclerosing cholangitis (PSC) is the commonest predisposing condition for cholangiocarcinogenesis, and the prevalence of CC in patients with PSC ranges from 9% to 23%, with a cumulative annual risk of 1.5% per year of the disease. 1 Other risk factors for cholangiocarcinogenesis are also associated with chronic biliary tract inflammation, including chronic choledocholithiasis, liver fluke infestation, hepatolithiasis, Caroli's disease, and hepatitis C viral infection. 1 It has been hypothesized that the increased risk of CC in these conditions occurs because of chronic epithelial inflammation leading to cell proliferation, along with enhanced production of endogenous mutagens in the bile. 1 However, the precise molecular mechanism that accounts for the development of CC on the basis of chronic biliary tract inflammation remains unsolved.

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Section: Methodsmentioning

confidence: 99%

Activation-induced cytidine deaminase links bile duct inflammation to human cholangiocarcinoma

et al. 2008

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“…First, we immunohistochemically stained the lung tissue specimens of six IPF patients, two nonspecific interstitial pneumonia (NSIP) patients, and a control (normal area distant from the lesion of surgically diagnosed organising pneumonia) for NGAL using a conventional method [5]. We also performed sequential immunofluorescent staining for NGAL and MMP-9.…”

Section: To the Editormentioning

confidence: 99%

Neutrophil gelatinase-associated lipocalin in idiopathic pulmonary fibrosis

Ikezoe

Handa

Mori

et al. 2014

European Respiratory Journal

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“…Mucin and p53 IHC staining was performed by the tyramide signal amplification-avidin-biotin complex method [24]. We used monoclonal antibodies against MUC5AC (Novocastra, Newcastle-upon-Tyne, UK; diluted 1:100) as a marker for gastric foveolar cells, MUC6 (Novocastra; 1:100) as a marker for gastric mucous neck cells and pyloric glands, MUC2 (Novocastra; 1:100) as a marker for intestinal goblet cells, CD10 (Novocastra; 1:100) as a marker for the small intestinal brush border, and p53 (Novocastra, NCL-p53-Do7).…”

Section: Mucin Phenotype and P53 Expressionmentioning

confidence: 99%

HER2 expression and its clinicopathological features in resectable gastric cancer

et al. 2012

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Background A recent randomized controlled trial (Trastuzumab for Gastric Cancer [ToGA] study) established standard scoring criteria of human epidermal growth factor receptor 2 (HER2) for gastric cancer and demonstrated the efficacy of trastuzumab for treating metastatic gastric cancer. The aim of the present study was to evaluate the frequency of HER2-positive cases by application of the standard criteria in patients with resectable gastric cancer and to examine the relationships between HER2 expression and prognosis, mucin phenotype, p53 status, and clinicopathological features. Methods A total of 213 patients were included in this retrospective study. All tumor samples were examined for HER2 expression by immunohistochemistry (IHC), HER2 amplification by in situ hybridization, and mucin and p53 expression by staining for CD10, MUC2, MUC5AC, MUC6, and p53.Results HER2-positive tumors were identified in 25 patients (11.7 %). HER2-positive cases were more frequently found in men, older patients, and in the intestinal histological type (P = 0.0048, 0.0309, and \0.0001, respectively). Although no association was found between HER2 overexpression and mucin phenotype, the expression of CD10 and p53 was significantly correlated with HER2 positivity (P = 0.0079 and 0.013). The overall survival of HER2-negative and -positive patients was not significantly different. However, in patients with stage III/ IV, overall survival was worse in HER2-positive patients (P = 0.0149). In a comparison between dual-color in situ hybridization (DISH) and fluorescence in situ hybridization (FISH), four IHC2?/3? cases that were DISH-positive were judged as negative by FISH. Conclusions Our study indicated that HER2 expression was less frequent in resectable gastric cancer than in metastatic gastric cancer. The impact of HER2 expression on survival was limited. DISH was superior to FISH for evaluating cases with limited HER2 expression.

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Application of tyramide signal amplification system to immunohistochemistry: A potent method to localize antigens that are not detectable by ordinary method

Cited by 117 publications

References 8 publications

Activation-induced cytidine deaminase links bile duct inflammation to human cholangiocarcinoma

Activation-induced cytidine deaminase links bile duct inflammation to human cholangiocarcinoma

Neutrophil gelatinase-associated lipocalin in idiopathic pulmonary fibrosis

HER2 expression and its clinicopathological features in resectable gastric cancer

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