Application of two types of Trypanosoma cruzi amastigotes of different virulence to ELISA for Chagas' disease.

Maldonado, M; Ichinose, Yuki; Samudio, Margarita; Arias, Antonieta De; Sakamoto, Makoto; Azorero, Ricardo Moreno; Kanbara, Hiroji

doi:10.2149/tmh1973.18.325

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1994

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“…This assay was performed with an ELISA kit as previously reported (Maldonado et al, 1990) . Antibody titers were determined as the highest dilution of the test serum which gave an optical density 0.8.…”

Section: Elisamentioning

confidence: 99%

The gelatin particle indirect agglutination test, a means of simple and sensitive serodiagnosis of Chagas' disease.

Yamashita

Watanabe

Maldonado

et al. 1994

Jap. J. Trop. Med. Hyg.

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Abstract:An indirect agglutination test using a Trypanosoma cruzi antigen-coated gelatin particles was employed to diagnose trypanosomiasis in Paraguay.Results with this test were quite comparable to those obtained with enzyme-linked immunosorbent assay (ELISA) . Furthermore, nonspecific reaction to the gelatin particles alone was not found in either acute or chronic infection. This method is more convenient than the ELISA, since the antigen-conjugated particles is stable for at least 1 year at 4°C and since the test itself is short and simple to perform and does not require specialized equipment.

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Section: Elisamentioning

confidence: 99%

The gelatin particle indirect agglutination test, a means of simple and sensitive serodiagnosis of Chagas' disease.

Yamashita

Watanabe

Maldonado

et al. 1994

Jap. J. Trop. Med. Hyg.

Self Cite

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show abstract

Application of two types of Trypanosoma cruzi amastigotes of different virulence to ELISA for Chagas' disease.

Abstract: Trypanosoma cruzi amastigotes derived from fibroblast cultures and from cell-free liquid medium were comparatively evaluated as solid-phase antigens of ELISA for Chagas' disease. Amastigotes were obtained from the high-and low-virulent clones of the Tulahuen strain and from the G-1 strain.

Cited by 1 publication

References 17 publications

The gelatin particle indirect agglutination test, a means of simple and sensitive serodiagnosis of Chagas' disease.

The gelatin particle indirect agglutination test, a means of simple and sensitive serodiagnosis of Chagas' disease.

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