Application of TMA-PEG to promote C-phycocyanin extraction from S. platensis in the PEG ATPS

Wang, Feng; Liu, Yanhua; Ma, Yong; Cui, Zhenggang; Shao, Lin-Lin

doi:10.1016/j.procbio.2016.11.006

Cited by 17 publications

(7 citation statements)

References 38 publications

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“…The influence of temperature on C‐PC extraction process has been reported by a few researchers . Silveira et al showed that a change in the extraction temperature from 23.6 to 41 °C reduced the purity and concentration of C‐PC, on average, 0.05 and 0.02 mg/mL, respectively, and 25 °C was the best temperature for C‐PC extraction.…”

Section: Resultsmentioning

confidence: 94%

“…When C-PC was extracted by aqueous two-phase system, the temperature of 25 C promoted a higher purity extract. 36 Pan-utai et al 37 verified that extraction at 25 C promoted the higher C-PC yield from S. platensis when compared to 4 and −20 C.…”

Section: Resultsmentioning

confidence: 99%

“…Martínez et al reported that an increase in temperature of the PEF from 25 to 40 °C influenced C‐PC extraction yield positively, whereas purity did not change. When C‐PC was extracted by aqueous two‐phase system, the temperature of 25 °C promoted a higher purity extract . Pan‐utai et al verified that extraction at 25 °C promoted the higher C‐PC yield from S. platensis when compared to 4 and −20 °C.…”

Section: Resultsmentioning

confidence: 99%

“…The influence of temperature on C-PC extraction process has been reported by a few researchers. 7,18,36,37 Silveira et al 18 showed that a change in the extraction temperature from 23.6 to 41 C reduced the purity and concentration of C-PC, on average, 0.05 and 0.02 mg/mL, respectively, and 25 C was the best temperature for C-PC extraction. Martínez et al 7 reported that an increase in temperature of the PEF from 25 to 40 C influenced C-PC extraction yield positively, whereas purity did not change.…”

Section: Resultsmentioning

confidence: 99%

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Cell pretreatment with ethylenediaminetetraacetic acid for selective extraction of C‐phycocyanin with food grade purity

Sala

Moraes

Kalil

2018

Biotechnology Progress

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C‐phycocyanin (C‐PC) is a natural blue dye, and depending on its purity, which is measured by the ratio between the absorbance of the chromophore (A620) and the absorbance of the proteins (A280), it can be used in food (purity > 0.7), cosmetics (purity > 1.5), and therapeutic treatments (purity > 4.0). Several physical, chemical, and enzymatic methods of extraction are reported, however, few are able to extract C‐PC with purity above 0.7. An innovative method of C‐PC extraction with food grade purity from wet Spirulina platensis biomass is proposed. The cells were pretreated with ethylenediaminetetraacetic acid and subsequent C‐PC extraction was performed with tris‐(hydroxymethyl) aminomethane‐SO4 buffer. C‐PC was released after 12 h of cell pretreatment. Six variables of the extraction process were evaluated. The extraction temperature significantly influenced C‐PC extraction yield and purity. In the best condition of cell pretreatment and extraction, C‐PC with purity of 1.0 and extraction yield of 129.0 mg/g could be obtained to be used as a food dye without any purification process. Lastly, an ultrafiltration process was integrated and C‐PC was concentrated 8.8‐fold, resulting in purity of 1.6 and recovery of 93.4%.

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Section: Resultsmentioning

confidence: 94%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 2 more Smart Citations

Cell pretreatment with ethylenediaminetetraacetic acid for selective extraction of C‐phycocyanin with food grade purity

Sala

Moraes

Kalil

2018

Biotechnology Progress

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“…The freezing-thawing method is predominantly applied in the laboratory-scale extraction of C-PC. Generally, the cell suspension is frozen (at −20 °C) and thawed (at 25 °C) repeatedly, four or five times [ 25 , 26 ]. The influence of the cycles of freezing-thawing on the yield of phycocyanin is shown in Figure 4 .…”

Section: Resultsmentioning

confidence: 99%

Application of an Ultrafine Shearing Method for the Extraction of C-Phycocyanin from Spirulina platensis

2017

Molecules

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Cell disruption is an important step during the extraction of C-phycocyanin from Spirulina platensis. An ultrafine shearing method is introduced and combined with soaking and ultrasonication to disrupt the cell walls of S. platensis efficiently and economically. Five kinds of cell disruption method, including soaking, ultrasonication, freezing-thawing, soaking-ultrafine shearing and soaking-ultrafine shearing-ultrasonication were applied to break the cell walls of S. platensis. The effectiveness of cell breaking was evaluated based on the yield of the C-phycocyanin. The results show that the maximum C-phycocyanin yield was 9.02%, achieved by the soaking-ultrafine shearing-ultrasonication method, followed by soaking (8.43%), soaking-ultrafine shearing (8.89%), freezing and thawing (8.34%), and soaking-ultrasonication (8.62%). The soaking-ultrafine shearing-ultrasonication method is a novel technique for breaking the cell walls of S. platensis for the extraction of C-phycocyanin.

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Extraction, purification and stability of C-phycocyanin from Arthrospira platensis

Fabre

Niangoran

Gaignard

et al. 2022

Eur Food Res Technol

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C-phycocyanin is a highly valuable phycobiliprotein from Arthrospira platensis. However, its extraction, purification and conservation currently limit its commercial use. We review here the most common techniques and less conventional methods. Simple incubation in phosphate buffer at neutral pH can give high yields (>100 mg/g) and even high protein purity (> 0.7) if the parameters (buffer concentration, temperature, incubation time, Arthrospira platensis. state etc.) are chosen correctly. This method is preferable to incubation in distilled water or acid solutions. Some mechanical, physical or thermal treatments can improve the extraction yield and accelerate the release of C-phycocyanin. Ultrasound-assisted extraction has been widely studied and probes generally give higher extraction yields than baths (> 100 mg/g) in less than 30 minutes. This technique can be coupled with others, such as freeze-thaw methods, to improve protein release. Indeed, freeze-thaw cycles are an efficient destructuring technique that can be used alone or as a pretreatment. Bead mills or pulsed electric fields can also be used to extract C-phycocyanin, but there is room for improvement in the choice of operating parameters. For purification of the extracted pigments, salting out or aqueous two-phase extraction can be used to increase phycocyanin grade. More advanced purification methods, mostly based on chromatography, can provide addition improvement. C-phycocyanin stability is dependent principally on pH and temperature, and is higher between pH 5 and pH 7 and at temperatures below 40°C, but the use of various preservatives or conditioning can increases its lifetime.

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Application of TMA-PEG to promote C-phycocyanin extraction from S. platensis in the PEG ATPS

Cited by 17 publications

References 38 publications

Cell pretreatment with ethylenediaminetetraacetic acid for selective extraction of C‐phycocyanin with food grade purity

Cell pretreatment with ethylenediaminetetraacetic acid for selective extraction of C‐phycocyanin with food grade purity

Application of an Ultrafine Shearing Method for the Extraction of C-Phycocyanin from Spirulina platensis

Extraction, purification and stability of C-phycocyanin from Arthrospira platensis

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