Application of an Ultrafine Shearing Method for the Extraction of C-Phycocyanin from Spirulina platensis

Abstract

Cell disruption is an important step during the extraction of C-phycocyanin from Spirulina platensis. An ultrafine shearing method is introduced and combined with soaking and ultrasonication to disrupt the cell walls of S. platensis efficiently and economically. Five kinds of cell disruption method, including soaking, ultrasonication, freezing-thawing, soaking-ultrafine shearing and soaking-ultrafine shearing-ultrasonication were applied to break the cell walls of S. platensis. The effectiveness of cell breaking was evaluated based on the yield of the C-phycocyanin. The results show that the maximum C-phycocyanin yield was 9.02%, achieved by the soaking-ultrafine shearing-ultrasonication method, followed by soaking (8.43%), soaking-ultrafine shearing (8.89%), freezing and thawing (8.34%), and soaking-ultrasonication (8.62%). The soaking-ultrafine shearing-ultrasonication method is a novel technique for breaking the cell walls of S. platensis for the extraction of C-phycocyanin.

Cell-wall disruption and characterization of phycocyanin from microalgae: Spirulina platensis using Catalytic ozonation

“…Microwave and supercritical CO2 requires very high investment costs, a lot of energy and resource competencies with less satisfactory results [6], [18]- [20]. On the other hand, solvent, acid/base and hydrolytic enzyme requires chemicals with high purity and setting conditions that are quite difficult to achieve [6], [11], [21], [22]. Low cell-wall disruption yield was confirmed using homogenization, ultrasonication, and fenton chemical [6].…”

Spirulina, The importance and Significance of Cloning

“…The presence of phycocyanin specific promoter sequence, which is the strongest promoter in Spirulina, and 20% of the spirulina proteins weight is expressed by this promoter. [10][11][12] 4. Given the limitations available, few studies have been performed on optimizing the Spirulina cloning pathway [19][20][21].…”

“…Spirulina has been known for many years for its high protein, vitamins, essential amino acids and essential fatty acids [9]. 55-70% of its dry matter is composed of spirulina protein [5,[10][11][12] and it is a rich source of vitamins especially B12 (commonly found in animal tissues) and a precursor of vitamin A (beta-carotene) and minerals especially iron. It contains a small amount of ^-linolenic acid (GLA) and also contains other beneficial herbal chemicals that are valuable to health [5].…”

POTENSI PIGMEN FIKOBILIPROTEIN SEBAGAI AGEN ANTIOKSIDAN DAN TOKSISITAS HAYATI DARI SIANOBAKTERIA Chroococcus turgidus (Potency of Phycobiliprotein Pigment as Antioxidant and Biological Toxicity Agents from Cyanobacteria Chroococcus turgidus)

“…Proses ekstraksi merupakan hal yang krusial sehingga kesesuaian metode dalam proses ini akan menentukan keberhasilan dan banyaknya yield pigmen fikobiliprotein yang diperoleh. Proses ekstraksi fikobiliprotein dari sianobakteria bisa saja sulit karena resistensi dinding sel dan ukuran sianobakteri yang kecil (Yu, 2017;Yu et al, 2017). Terdapat beberapa metode dalam ekstraksi fikobiliprotein, salah satunya menggabungkan pemecahan dinding sel dan ekstraksi fikobiliprotein yang larut dalam air.…”