Application of Tissue Microarrays to Study the Influence of Dexamethasone on NF-κB Expression of Pancreas in Rat with Severe Acute Pancreatitis

Zhang, Xi Ping; Zhang, Ling; Xu, Hong; Xu, Yan; Cheng, Qi; Wang, Jian Mei; Shen, Haikang

doi:10.1007/s10620-007-9867-4

Cited by 16 publications

(17 citation statements)

References 27 publications

(28 reference statements)

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“…Although enormous studies of SAP pathogenesis have been conducted, its exact mechanism remains unclear. Presently, researchers have proven that the activation of NF-κB plays an extremely important role in the development of SAP [5,13]. As one important signaling molecule, it could modulate the expression of numerous genes, such as genes of cytokines, chemotactic factor, macrophage chemotactic peptide, cellular adhesion molecule, growth factor, immune receptor, and acute phase reactive protein [14][15][16][17].…”

Section: Discussionmentioning

confidence: 99%

Role of Intrapulmonary Expression of Inducible Nitric Oxide Synthase Gene and Nuclear Factor κB Activation in Severe Pancreatitis-associated Lung Injury

et al. 2010

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The aim of this study is to explore the relationship of intrapulmonary activation of nuclear factor-kappaB (NF-kappaB) and the expression of inducible nitric oxide synthase (iNOS) mRNA with pulmonary injury in rats with severe acute pancreatitis (SAP). Fifty-four Sprague Dawley rats were randomly divided into three groups: sham operation (control) group (n = 18), SAP group (n = 18), and pyrrolindine dithiocarbamate (PDTC) pretreated group (n = 18). A SAP model was induced by retrograde injected 5% sodium taurocholate into the bile-pancreatic duct (1 ml/kg). PDTC-pretreated SAP rats were given 100 mg/kg body weight PDTC intraperitoneally before pancreatitis was induced. Six rats from each group were sacrificed at 3, 6, and 12 h after modeling. Activation of NF-kappaB in pulmonary tissues and pancreas tissues was detected by immunohistochemical methods. Intrapulmonary expression of iNOSmRNA was assayed by fluorogenic quantitative reverse transcription polymerize chain reaction. The expression of NF-kappaB in the SAP group in pulmonary tissues was enhanced significantly at any measure point compared with control group (58.4 +/- 10.8 vs. 3.8 +/- 1.8, 119.8 +/- 17.8 vs. 5.2 +/- 2.4, and 90.2 +/- 14.4 vs. 4.7 +/- 2.2, P < 0.01). But the expressions of NF-kappaB in the PDTC group were significantly lower than those in SAP group (54.3 +/- 9.6 vs. 58.4 +/- 10.8, 93.9 +/- 7.9 vs. 119.8 +/- 17.8, and 82.2 +/- 13.3 vs. 90.2 +/- 14.4, P < 0.05). The number of positive cells in SAP group and PDTC group reached its peak at 6 h and then declined. The expression of iNOSmRNA in PDTC groups was significantly weaker than that in SAP group (2.0 +/- 0.8 vs. 2.2 +/- 1.9, 2.4 +/- 1.2 vs. 4.6 +/- 1.8, and 1.5 +/- 0.8 vs. 3.2 +/- 1.5, P < 0.05). The activation of NF-kappaB may be involved in the SAP lung injury through regulating the expression of iNOSmRNA. PDTC might inhibit the activation of NF-kappaB and then reduce the expression of iNOSmRNA and effectively alleviate the severity of lung injury.

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Section: Discussionmentioning

confidence: 99%

Role of Intrapulmonary Expression of Inducible Nitric Oxide Synthase Gene and Nuclear Factor κB Activation in Severe Pancreatitis-associated Lung Injury

et al. 2010

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“…After inserting a segmental epidural catheter into the duodenum cavity via the hole, it was inserted into the biliary-pancreatic duct, in the direction of the papilla, in a retrograde position. This was followed by retrograde transfusion of 3.5% sodium taurocholate 0.1 mL/100 g by microinjection pump at the speed of 0.2 mL/min, and then the hole in the duodenum lateral wall was sutured [2][3][4][5][6] .…”

Section: Animal Grouping and Rat Sap Model Preparationmentioning

confidence: 99%

Influence of dexamethasone on mesenteric lymph node of rats with severe acute pancreatitis

Zhang¹,

Xu²,

Jiang³

et al. 2008

WJG

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AIM:To study the influence and mechanisms of dexamethasone on mesenteric lymph node of rats with severe acute pancreatitis (SAP). METHODS:The SAP rats were assigned to model, treated or sham-operated groups. The mortality, pathological changes of mesenteric lymph nodes, expression levels of NF-kB, P-selectin, Bax, Bcl-2 and caspase-3 protein and changes in apoptotic indexes in lymph nodes were observed at 3, 6 and 12 h after operation. The blood levels of endotoxin, superoxide dismutase (SOD), malondialdehyde (MDA), and endothelin-1 (ET-1) in blood were determined. RESULTS: SOD content, expression of Bax protein and apoptotic index were significantly higher in the treated group than in the model group at different time points (P < 0.05 or P < 0.01). Other blood-detecting indexes and histopathological scores of mesenteric lymph nodes were lower in the treated than in the model group (P < 0.05, P < 0.01 or P < 0.01). NF-kB protein expression was negative in all groups. Comparing P-selectin and caspase-3 expression levels among all three groups, there was no marked difference between the model and treated group. CONCLUSION: Dexamethasone can protect mesenteric lymph nodes. The mechanism may be by reducing the content of inflammatory mediators in the blood and inducing lymphocyte apoptosis.

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“…Additionally, micro-array technology was applied to immunohistochemistry and TUNEL assay, thus saving a lot of manpower and material resources, improving the efficiency of experiments, reducing experimental error, providing ease of designing experimental controls, and bringing benefits to the preservation of the original wax blocks [28]. Salvia miltiorrhizae, is able to provide some protection against lung injury in SAP and OJ rats though its effect is limited.…”

Section: Discussionmentioning

confidence: 99%

Effect of Salvia miltiorrhizae on Pulmonary Apoptosis of Rats with Severe Acute Pancreatitis or Obstructive Jaundice

Zhang

Chengjun

et al. 2009

Inflammation

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To investigate the effect of apoptosis about Salvia miltiorrhizae injection on the lungs of SAP and OJ rats. Total 288 rats were used for SAP-associated experiments and OJ-associated experiments, respectively. The rats were randomly divided into sham-operated, model control and treated group. According to the difference of time points after operation, the SAP rats in each group were subdivided into 3, 6 and 12 h groups while the OJ rats were divided into 7, 14, 21 and 28 days groups. The pathological changes, expression levels of Bax protein and apoptotic indexes in the lungs of SAP or OJ rats were observed, and the mortality rates of SAP or OJ rats were recorded, respectively. The numbers of dead SAP and OJ rats in treated groups declined. The pathological changes in the lungs of SAP or OJ rats in treated groups were relieved to varying degrees. There was no marked difference in pathological severity scores and the positive staining intensity of Bax protein between treated groups and model control groups (all P > 0.05). Salvia miltiorrhizae has some protective effect on the lungs of rats with SAP or OJ which may be related apoptosis although our results can not find significant difference between treated groups and model control groups.

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Application of Tissue Microarrays to Study the Influence of Dexamethasone on NF-κB Expression of Pancreas in Rat with Severe Acute Pancreatitis

Cited by 16 publications

References 27 publications

Role of Intrapulmonary Expression of Inducible Nitric Oxide Synthase Gene and Nuclear Factor κB Activation in Severe Pancreatitis-associated Lung Injury

Role of Intrapulmonary Expression of Inducible Nitric Oxide Synthase Gene and Nuclear Factor κB Activation in Severe Pancreatitis-associated Lung Injury

Influence of dexamethasone on mesenteric lymph node of rats with severe acute pancreatitis

Effect of Salvia miltiorrhizae on Pulmonary Apoptosis of Rats with Severe Acute Pancreatitis or Obstructive Jaundice

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