Application of Time-Resolved Fluorometry of Lanthanide in Glycobiology

Development of luminescent Sm(III) chelates containing hexadentate to nonadentate ligands: synthesis, photophysical properties and coupling to biomolecules

“…Lanthanide chelates have been widely used in bioaffinity assays during the last two decades [1][2][3][4].…”

Carbohydrate Microarray

“…However, sometimes the sensitivity is relatively low toward the use of low molecular-weight carbohydrates, though the problem can be overcome by labeling sugars with heavy metal ions [17]. In addition, fluorescence polarization and two-photon fluorescence correlation have been applied to study lectin-glycan interactions [18][19][20][21]. The most applicable technique is carbohydrate microarrays which immobilize oligosaccharides to a solid supports are developed and widely used to measure the carbohydrate binding properties of proteins, cells, or viruses [22][23][24][25].…”