Application of the redox potential measurementbased rapid method in the microbial hygienic control

Erdősi, O.; Szakmár, K.; Reichart, O.; Székely-Körmöczy, P.; Laczay, Péter

doi:10.1556/aalim.2011.0005

Cited by 13 publications

(9 citation statements)

References 5 publications

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“…Redox potential was measured by a multichannel reader, a device named 'MicroTester' that monitored redox potential in maximum 64 samples simultaneously (4 blocks of 16 tube test units) and registered values using the manufacturer's Windows-based software (Erdősi et al, 2012). The parameter of decision threshold (DT) was set to be -0.6 mV/min drop rate in this study; this DT has been established previously (Erdősi et al, 2012).…”

Section: Redox Potential Measurement Setupmentioning

confidence: 99%

“…We used a highly selective media, the Z-broth that provides growth support to only P. aeruginosa without any inhibitory substance (Szita et al, 2007). Z-broth has been shown to be a suitable means of accelerated selective P. aeruginosa detection in a study where bacterial growth was detected by impedance measurement (Erdősi et al, 2012). However, impedance/conductance measurements can be in error due to even slight temperature variations and they also demand high hardware cost and skilled personnel.…”

Section: Introductionmentioning

confidence: 99%

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Semiautomated detection ofPseudomonas aeruginosafrom diverse water samples using highly selective Z-broth and a redox potential monitor

Szita

Szakmár

Bernáth

et al. 2020

Preprint

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Highlights •A method was applied for detection of viable Pseudomonas aeruginosa in 739 water samples. •Redox potential monitoring of Z-broth cultures exclusively registered growth of P. aeruginosa. •The method is highly selective, reliable and partly automated, with lower labor costs. •The method is suited for testing of water and hospital environments for P. aeruginosa. AbstractPseudomonas aeruginosa is a facultative bacterial pathogen with increasing public health risk potential. Contaminated water in hospital environments is a growing cause of multidrugresistent P. aeruginosa nosocomial infections that are life-threatening and are costly to treat. P. aeruginosa is common in natural water bodies, but it is not unusual in drinking water and has been detected in bottled water, also. Suppliers in Europe must eliminate live forms of the bacterium in drinking water to meet human consumption requirements. Laboratory testing for the presence of viable P. aeruginosa is mostly done manually using culture media but conductance/impedimetry measurements for detection are also available. In order to improve expedience and cost efficiency as well as to automate the detection and the data registration, we applied the highly selective Z-broth culture media and redox potential monitoring to detect P. aeruginosa from water. The Z-broth is based on only a few, stable chemicals that provide consistency of quality and long shelf life. It limits growth to P. aeruginosa and, thus, eliminates the need for subsequent microbiological identification steps of the European Standard procedure defined in ISO 16 266:2006ISO 16 266: (2018. A redox potential monitor was used in this work, that simultaneously recorded 64 sample curves and automatically marked positive samples reliably within 24 hours after sample initialization. In comparison, the standard method requires additional tests that prolongs identification to some days. Practical applicability and reliability of the method in this paper was demonstrated by testing a total of 739 water samples of which 222 were tap water, 342 well water and 145 had been taken from swimming pools.It is considered this method is well suited to process large numbers of samples for purposes of detecting P. aeruginosa contamination with relative ease, little cost in shortened time.Using Z-broth in combination with redox potential monitoring can be recommended for central laboratories for routine testing of drinking water for live P. aeruginosa presence. As well, using this method for testing of fluids and surfaces for P. aeruginosa contamination would be advantageous in such environments as the hospital industry, swimming pools and spas, where cleaning routines and P. aeruginosa transmission prevention protocols could be made much more resultful and cost-effective due to ease and speed of processing both spot check and water samples.

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Section: Redox Potential Measurement Setupmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Semiautomated detection ofPseudomonas aeruginosafrom diverse water samples using highly selective Z-broth and a redox potential monitor

Szita

Szakmár

Bernáth

et al. 2020

Preprint

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“…Furthermore, it can determine whether or not a unit ensures appropriate hygienic conditions during its activity. In our present work, besides conventional methods, the MicroTester instrument (Erdősi et al, 2012;Reichart et al, 2007), which indicates the growth of bacteria through a change in redox potential, was used.…”

Section: Introductionmentioning

confidence: 99%

“…Correspondingly, the MicroTester instrument enables the use of culture media for the standard microbiological processes as well. The shape of the redox curve is related to different microbial groups that differ from one another; by this means, in certain cases, the method makes it possible for the rough identification of the microbial group (Erdősi et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Hygiene assessments of school kitchens based on the microbiological status of served food

Tóth¹,

Szakmár

Dunay

et al. 2018

Acta Sci Pol Technol Aliment

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“…The instrumental procedure based on redox potential measurement was originally developed for rapid determination of viable count of several microorganisms in water, milk, foods, hygienic samples, etc. (rEichart et al, 2007;Erdősi et al, 2012). The aim of the present work was to develop a protocol for rapid detection of Salmonella by the combination of the redox potential based and real-time PCR methods in a simple time and cost-effective manner.…”

mentioning

confidence: 99%

Rapid detection ofSalmonellain food by redox-potential measurement based method combined with real-time PCR

Erdősi¹,

Szakmár²,

Reichart³

et al. 2014

Acta Alimentaria

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The classical ISO (2002) standard as reference method and the combination of redox potential measurement with real-time PCR technique were applied to detect Salmonella in milk, egg, broiler meat, and artificially contaminated egg samples. Food samples of 25 g were homogenized in 225 ml of RVS broth to prepare the basic suspension of the comparative tests. In the combined method the redox potential measurement technique serves as the selective enrichment system of the real-time PCR equipment. The reliable screening of Salmonella-free, negative samples by the redox potential measurement technique needed only 24 h. These negative samples determined by the PCR and the classical standard method in all cases proved to be negative as well. In case of positive redox result the Salmonella from the enriched suspension of the redox test-cell was identified by real-time PCR in 3 hours, instead of the conventional biochemical identification. Comparing our protocol to the ISO (2002) standard method, the total detection time of Salmonella presence/absence was less than 24 h contrary to the 114 h of the conventional method.

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Application of the redox potential measurementbased rapid method in the microbial hygienic control

Cited by 13 publications

References 5 publications

Semiautomated detection ofPseudomonas aeruginosafrom diverse water samples using highly selective Z-broth and a redox potential monitor

Semiautomated detection ofPseudomonas aeruginosafrom diverse water samples using highly selective Z-broth and a redox potential monitor

Hygiene assessments of school kitchens based on the microbiological status of served food

Rapid detection ofSalmonellain food by redox-potential measurement based method combined with real-time PCR

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