Application of the multisubstrate testing method to the characterization of marine microbial fouling communities on metals and alloys

Беленева, И. А.; Kharchenko, U. V.; Kovalchuk, Yu. L.

doi:10.1134/s1063074010020100

Cited by 5 publications

(1 citation statement)

References 4 publications

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“…According to the mentioned method, the functional properties of the soil communities are studied by its reaction (direct microscopy) by applying into the soil samples the different artificial substrates, that is, glucose, mannitol, asparagines, citrate, succinate, etc. Further, the method proposed by Yakushev and Byzov (2008), Beleneva et al (2010), etc. is commonly used in soil and marine microbiology for studying the development of specific active-functioning microbial communities, depending on the applied substrate.…”

Section: Introductionmentioning

confidence: 99%

Control of urban soils toxicity by evaluation of bacterial community changes

Mynbayeva¹,

Seilova²,

Amirasheva³

et al. 2014

Afr. J. Microbiol. Res.

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Since 1990, Almaty City's soils has been considerably polluted by heavy metals, which resulted in its' unhealthy ecological conditions. Almaty soils are light chestnut soils with the following physical and chemical properties: packing, 1.6±0.5 g•cm-3 ; available air space, 36.1 ± 9.4%; salt composition of the soil aqueous extract, 0.12±0.04%; hygroscopity (water-absorbing quality), 17.2±1.8, quantity of exchangeable cations, mg• eqv•100 g soil-59.1±4.2 Ca 2+ , 8.4±0.5 Mg 2+ , 0.6±0.1 K + , 0.3±0.04 Na + ; humus, 1.6±0.4%; С total , 0.9±0.2%; N total , 0.14±0.02. The purpose of this research was to determine the degree of soil toxicity by the method of multisubstrate testing (MST) for the integral studies of Almaty city soils' bacterial communities, polluted by heavy metals (HM). This method measures soil bacterial communities' activity in terms of its uptake intensity of 47 different organic substrates. Their stunted condition was determined through a decreased amount of consumed substrates ("N") and urban soils' metabolic activity decay: total biomass ("W"), load upon ecosystem or disturbance "d" coefficient. It was found that: "d" ecoefficient equaled 1.0 for the baseline soil and "d" ratio equaled 0.6 for urban soils. Ecological soil state was assessed using MST method, looking into soil bacterial communities' activity in terms of its uptake intensity of different organic substrates. This method was developed in the last decade of the twentieth century for the purpose of investigation of soil microbial communities' state. Thus, urban soil samples displayed worse uptake intensity than the baseline soil. Hence, the MST method may be used as the original method of evaluation of urban soil toxicity degree.

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Section: Introductionmentioning

confidence: 99%