Application of the Multiplex PCR Method for Discrimination of Artemisia iwayomogi from Other Artemisia Herbs

Molecular Authentication of 21 Korean Artemisia Species (Compositae) by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Based on trnL-F Region of Chloroplast DNA

“…Furthermore, Artemisia herbs are generally available in dried or sliced forms in the market. 9,10) For these reasons, Artemisia herbs are likely to be traded under their medicinal names in the market and their names may be used incorrectly or without regard to efficacy for some diseases.…”

“…Various molecular techniques detecting different genetic information have been employed for identification of plant species. Lee et al 9,10) developed the sequence characterized amplified region (SCAR) marker for discriminating 'Aeyup' and 'Haninjin' from other Artemisia herbs. However, more DNA markers are needed to develop Artemisia herbs industrially and to provide consumers with correct materials for medicinal purposes.…”

Discrimination of Kalopanax pictus from its varieties and other ‘Kalopanacis Cortex’ plants by multiplex polymerase chain reaction (PCR)

“…To discriminate medicinal herbal materials, subjective methods frequently based on the morphological features of plants and/or the observations of trained individuals are generally applied (Lee et al, 2008). However, these methods are likely to be inappropriate to identify samples of stem barks that morphologically resemble each other, especially when the stem barks are in a dried and sliced state.…”

“…Furthermore, we designed primer sets to amplify the DNA markers of Z. ailanthoides, E. variegata, and common DNA markers of both K. pictus and its varieties. In previous studies, medicinal plants such as Artemisia iwayomogi and A. asphodeloides were discriminated efficiently from taxonomically related plants by using the multiplex PCR method, which is more accurate and efficient than conventional PCR: conventional PCR amplifies only 1 DNA marker of a specific plant in each PCR process (Herve et al, 1999;Jigden et al, 2010;Lee et al, 2008). Using our designed primer sets, we aimed to develop a specific multiplex PCR method to not only discriminate K. pictus from its varieties and other KC plants but also identify the KC plants in a single PCR amplification.…”

Discrimination of three Scrophularia plants utilizing ‘Scrophularia Radix’ by DNA markers based on internal transcribed spacer (ITS) sequences

“…However, these methods are not always sufficient for the identification of herbs that morphologically resemble each other, particularly when the herbs are in a dried and/or sliced state, the common forms of herbs when they are traded (Lee et al, 2008). To overcome the problem of these methods, various molecular biology techniques utilizing different genetic information of organism, are used for the discrimination of plant species (McClelland and Welsh, 1994).…”