Application of the MAIEA Assay to the Kell Blood Group System

Petty, A. C.; Daniels, Geoff; Tippett, Patricia

doi:10.1111/j.1423-0410.1994.tb00313.x

Cited by 24 publications

(10 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Antibodies to most of these and other Kell antigens have been used in this study to provide control data with which to compare the results obtained with TOU and IAN. The re sults we obtained with antibodies to K (KEL1), k (KEL2), Kpa (KEL3), Kpb (KEL4), Ku (KEL5), Jsa (KEL6), Jsb (KEL7), Wka (KEL17) and K18 (KEL18) were in agreement with Petty [7,9]. Although Ula was not included in this study, Petty has shown that this antigen is close to the BRIC107 epitope [9], In addition, we have confirmed, using the MAIEA assay, that the low-incidence antigens K23 (KEL23) [12] and K24 (KEL24) [13] are carried on the Kell glycoprotein.…”

Section: Discussionsupporting

confidence: 74%

“…The incompat ibility of the RBCs from all available TOU family members with TOU serum and the unavailability of I AN family mem- 58 Jones/Reid/Oyen/Hams/Moscarelli/Co/ Leger/Beal/Cardillo bers has meant that we have been unable to show that this antigen is inherited and thus an ISBT number cannot cur rently be allocated to the antigen. The relative locations of the following Kell antigens us ing the MAIEA assay have previously been described [7,9] : K/k (KEL1/KEL2), K22 (KEL22), Ul" (KEL10), Jsa/Jsb (KEL6/KEL7), K14 (KEL14), Kpc (KEL21), K18 (KEL18), Kpa (KEL3), Kpb (KEL4), Ku (KEL5) and Wka (KEL17). Antibodies to most of these and other Kell antigens have been used in this study to provide control data with which to compare the results obtained with TOU and IAN.…”

Section: Discussionmentioning

confidence: 99%

“…Results were expressed as a ratio of absorb ance obtained with antigen-positive and antigen-negative RBCs tested in parallel. As recommended by Petty [9], a ratio of greater than 2:1 was considered as a positive result.…”

Section: Methodsmentioning

confidence: 99%

“…Antibodies to Kpa (KEL3 ), Kpb (KEL4) and Kpc (KEL21) gave similar re action patterns showing complete inhibition with BRIC68 and BRIC203 and high ratios with BRIC107 and BRIC18. BRIC203 has been described as anti-Kpbc [9] and it is there- RBCs from TOU were not agglutinated by IAN semm and RBCs from IAN were not agglutinated by TOU serum. Thus, TOU and IAN are mutually compatible and we pro pose to name the antigen TOU.…”

Section: Maiea Assaymentioning

confidence: 99%

See 3 more Smart Citations

A Novel Common Kell Antigen, TOU, and Its Spatial Relationship to Other Kell Antigens

Jones¹,

Reid²,

Oyen³

et al. 1995

Vox Sanguinis

View full text Add to dashboard Cite

A 47-year-old native American (TOU) was admitted to hospital for hip surgery. His serum agglutinated all red blood cells (RBCs) tested except Ko and DTT- treated RBCs and was weakly reactive with RBCs known to have a weak expression of Kell antigens, namely Kmod, McLeod, Kp(a+b-) (KEL:3,-4) and K:-13 (KEL:-13) phenotypes. RBCs from three siblings, a son and a daughter were incompatible with TOU’s antibody. TOU’s RBCs had the common Kell phenotype: K- k+ Kp(a-b+c-) Ku+ Js(a-b+) Ul(a-) K:ll, -17 K:14,-24 K: 12,13,18,19, 22,-23 (KEL:-1,2,-3,4,5,-6,7,-10,11,12,13,14,-17,18,19,-21,22,-23,-24). Since TOU’s RBCs were not agglutinated by an unidentified Kell-related antibody (IAN), tests were performed to show that TOU and IAN were mutually compatible. IAN is a Latino female hospitalised for a hysterectomy. The TOU antigen was shown to be located on the Kell glycoprotein by a monoclonal antibody immobilisation of erythrocyte antigen (MAIEA) assay. The unique pattem of reactivity obtained with TOU and IAN antibodies using this assay indicated the TOU epitope to be in an area remote from other Kell antigens, namely K, k, Kp^a, Kp^b, Kp^c, Ku, Js^a, Js^b Ul^a, K11, K12, K13, K14, Wk\ K18, K19, K22 and K24 (KEL1, KEL2, KEL3, KEL4, KEL5, KEL6, KEL7, KEL11, KEL12, KEL13, KEL14, KEL17, KEL18, KEL19, KEL21, KEL22 and KEL24) but close to the low-incidence antigen K23 (KEL23). Investigation of antibodies to previously identified antigens on the Kell glycoprotein by MAIEA using the mouse monoclonal antibodies BRIC18, BRIC68, BRIC107 and BRIC203 has identified six patterns of reactivity and has provided evidence for Kp^c being located in the same region as Kp^a and Kp^b.

show abstract

Section: Discussionsupporting

confidence: 74%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Maiea Assaymentioning

confidence: 99%

See 2 more Smart Citations

A Novel Common Kell Antigen, TOU, and Its Spatial Relationship to Other Kell Antigens

Jones¹,

Reid²,

Oyen³

et al. 1995

Vox Sanguinis

View full text Add to dashboard Cite

show abstract

“…While the manuscript was being reviewed, Petty et al [15] published results of the MAIEA assay and multiple an tibodies in the Kell blood group system. Our results are in agreement with their report.…”

Section: Discussionmentioning

confidence: 99%

K12 Is Located on the Kell Blood Group Protein in Proximity to K/k and Js^a/Js^b

Reid¹,

Øyen²,

Redman³

et al. 1995

Vox Sanguinis

View full text Add to dashboard Cite

K12 is a high-prevalence antigen, with no known antithetical partner, that is associated with the Kell blood group system. We report studies on the 5th propositus (MS) with the K:-12 phenotype and the second case to show that the K12 is inherited. The anti-K12 in his serum did not destroy antigen-positive incompatible red cells transfused on at least 3 occasions over 6 years. Red cell membranes from MS possessed the Kell protein that was indistinguishable from control membranes. K12 antigen was shown by immunoprécipitation to be on a protein with an apparent molecular mass of 93,000 and by monoclonal antibody immobilization of erythrocyte antigens (MAIEA) assay to be on the Kell protein in proximity to K/k and Js^a/Js^b antigens. These data remove the K:-12 phenotype from its current Kell-related (or para Kell) status and elevates the K12 antigen to a bona fide member of the Kell blood group system.

show abstract

Other Red Cell Antigens

Klein¹,

Anstee²

2005

Mollison's Blood Transfusion in Clinical Medicine

View full text Add to dashboard Cite

Application of the MAIEA Assay to the Kell Blood Group System

Cited by 24 publications

References 17 publications

A Novel Common Kell Antigen, TOU, and Its Spatial Relationship to Other Kell Antigens

A Novel Common Kell Antigen, TOU, and Its Spatial Relationship to Other Kell Antigens

K12 Is Located on the Kell Blood Group Protein in Proximity to K/k and Js^a/Js^b

Other Red Cell Antigens

Contact Info

Product

Resources

About