Application of the holographic phase contrast method for 3D imaging of phase micro-objects

We have developed the digital holographic interference microscope (DHIM) for real time three -dimensional (3-D) imaging and quantitative study of phase microscopic objects (MOs). The instrument integrates holographic interference microscope with digital processing of interferograms. The experimental procedure, allowing calculating microscopic objects thickness and 3-D imaging from the measured phase shift, is presented. The method has been applied to study 3-D morphology of red blood cells in norm, in pathology and under influence of physical and chemical factors in vivo. It has been found that morphologic changes of erythrocyte population had nonspecific character. Our investigations have proved that erythrocytes 3-D morphology reflects the state of a living organism and the level of its biological response on different external factors influence.

show abstract

<title>Erythrocytes three-dimensional morphology by digital holographic interference microscopy</title>

Tishko

Titar

Tishko

2008

SPIE Proceedings

View full text Add to dashboard Cite

show abstract

Shot noise, refractive index tomography and aberrations estimation in digital holographic microscopy

Charrière¹

2007

View full text Add to dashboard Cite

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

hi@scite.ai

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Application of the holographic phase contrast method for 3D imaging of phase micro-objects

Cited by 2 publications

References 1 publication

<title>Erythrocytes three-dimensional morphology by digital holographic interference microscopy</title>

<title>Erythrocytes three-dimensional morphology by digital holographic interference microscopy</title>

Shot noise, refractive index tomography and aberrations estimation in digital holographic microscopy

Contact Info

Product

Resources

About