2010
DOI: 10.1016/j.scitotenv.2009.09.056
|View full text |Cite
|
Sign up to set email alerts
|

Application of the denaturing gradient gel electrophoresis (DGGE) technique as an efficient diagnostic tool for ciliate communities in soil

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1

Citation Types

1
12
0

Year Published

2012
2012
2023
2023

Publication Types

Select...
5
4

Relationship

1
8

Authors

Journals

citations
Cited by 42 publications
(13 citation statements)
references
References 39 publications
(43 reference statements)
1
12
0
Order By: Relevance
“…PCR amplification and DGGE of whole coral samples. From crushed and extracted samples, ciliate 18S rRNA genes were amplified with an un‐nested PCR approach (Jousset et al ., 2010). Three 10 µl replicates of each sample were run using 8 ng of DNA product (PCR mixture as above) with the forward primer CilF (5′‐TGGTAGTGTATTGGACWACCA‐3′) with a 36 bp GC clamp (Muyzer and Smalla, 1998) attached to the 5′ end and CilDGGE‐r (5′‐TGAAAACATCCTTGGCAACTG‐3′).…”
mentioning
confidence: 99%
“…PCR amplification and DGGE of whole coral samples. From crushed and extracted samples, ciliate 18S rRNA genes were amplified with an un‐nested PCR approach (Jousset et al ., 2010). Three 10 µl replicates of each sample were run using 8 ng of DNA product (PCR mixture as above) with the forward primer CilF (5′‐TGGTAGTGTATTGGACWACCA‐3′) with a 36 bp GC clamp (Muyzer and Smalla, 1998) attached to the 5′ end and CilDGGE‐r (5′‐TGAAAACATCCTTGGCAACTG‐3′).…”
mentioning
confidence: 99%
“…T-RFs that were enriched in heavy fractions of 13 C treatments but not in 12 C treatments were regarded as 13 C labeled, even if those T-RFs occurred in the light fractions of 13 C treatments. The eukaryotic primer set, as well as primers targeting two ubiquitously distributed bacterivorous flagellate groups, i.e., Chrysophyceae and Kinetoplastidae, and the ciliates (24,25) were applied. T-RFLP analysis was conducted as described in a previous study (26) using the restriction enzymes MspI, RsaI, MseI, and Cfr and RsaI for 18S rRNA amplicons of total eukaryotes, ciliates, Kinetoplastidae, and Chrysophyceae, respectively.…”
Section: Identification Of 13 C-labeled 18s Rrna Phylotypes By T-rflpmentioning
confidence: 99%
“…We analyzed the ciliate community using DGGE modified after Jousset et al (2010). A fragment of the 18S rRNA gene was amplified from extracted DNA by seminested PCR.…”
Section: Dgge Analysis Of Ciliate Communitymentioning
confidence: 99%
“…Ciliates are a monophyletic group of protists, which allows us to use a molecular approach based on the SSU rRNA gene to analyze the community structure of soil ciliates (Lara et al 2007;Jousset et al 2010;Shimano et al 2012). One of the greatest advantages of studying ciliates is that some high-level clades (i.e., orders and classes) are characterized by common metabolic and life-history features (Lara and Acosta-Mercado 2012), which rationalizes the use of molecular-based information of the ciliate communities as soil bioindicators.…”
Section: Introductionmentioning
confidence: 99%