Application of supercritical gel drying method on fabrication of mechanically improved and biologically safe three-component scaffold composed of graphene oxide/chitosan/hydroxyapatite and characterization studies

Yılmaz, Pelin Köseoğlu; Er, Elif Öztürk; Bakırdere, Sezgin; Ülgen, Kutlu Ö.; özbek, Belma

doi:10.1016/j.jmrt.2019.08.043

Cited by 29 publications

(14 citation statements)

References 61 publications

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“…Moreover, scaffolds composed of CS, GO and HAp improved MC3T3-E1 cell viability compared to the material consisting only CS and GO. However, this effect is strictly related to the component ratio in the scaffold [ 36 ]. Thus, our results indicate that PEG may also improve the cell proliferation and may potentially be used as an additive for preparation of the gradient hydrogels dedicated for tissue engineering.…”

Section: Resultsmentioning

confidence: 99%

Gradient Chitosan Hydrogels Modified with Graphene Derivatives and Hydroxyapatite: Physiochemical Properties and Initial Cytocompatibility Evaluation

Kosowska

Domalik-Pyzik

Sekuła-Stryjewska

et al. 2020

IJMS

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In this study, we investigated preparation of gradient chitosan-matrix hydrogels through a novel freezing–gelling–thawing method. The influence of three types of graphene family materials (GFM), i.e., graphene oxide (GO), reduced graphene oxide (rGO), and poly(ethylene glycol) grafted graphene oxide (GO-PEG), as well as hydroxyapatite (HAp) on the physicochemical and biological properties of the composite hydrogels was examined in view of their potential applicability as tissue engineering scaffolds. The substrates and the hydrogel samples were thoroughly characterized by X-ray photoelectron spectroscopy, X-ray diffractometry, infrared spectroscopy, digital and scanning electron microscopy, rheological and mechanical analysis, in vitro chemical stability and bioactivity assays, as well as initial cytocompatibility evaluation with human umbilical cord Wharton’s jelly mesenchymal stem cells (hUC-MSCs). We followed the green-chemistry approach and avoided toxic cross-linking agents, using instead specific interactions of our polymer matrix with tannic acid, non-toxic physical cross-linker, and graphene derivatives. It was shown that the most promising are the gradient hydrogels modified with GO-PEG and HAp.

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Section: Resultsmentioning

confidence: 99%

Gradient Chitosan Hydrogels Modified with Graphene Derivatives and Hydroxyapatite: Physiochemical Properties and Initial Cytocompatibility Evaluation

Kosowska

Domalik-Pyzik

Sekuła-Stryjewska

et al. 2020

IJMS

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“…Out of 71 relevant article titles collected from SCOPUS, PubMed, and Google Scholar, only 36 were included after screening the title, abstract, and content for in vitro degradation and cytotoxicity testing for the biocompatibility of eggshell-based hydroxyapatite. The articles came from several countries, such as India [ 11 , 14 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 ], Bangladesh [ 41 , 42 ], China [ 43 ], Korea [ 25 , 44 , 45 ], Turkey [ 46 , 47 , 48 ], Thailand [ 49 , 50 ], Brazil [ 26 ], Italy [ 51 ], USA [ 52 ], Romania [ 2 ], Vietnam [ 53 , 54 ], Colombia [ 55 ], and Egypt [ 56 ]. The study selection of the articles included in the systematic review is shown in Figure 1 .…”

Section: Resultsmentioning

confidence: 99%

“…The addition of Si and Mg to the hydroxyapatite composite can increase the viability and proliferation of cells in osteoblasts [55]. Yilmaz et al [47] also tested HA/CS/GO composites, in which the addition of graphite was shown to increase the osteoconductivity of the materials. In addition, zirconia [31] and wollastonite compounds obtained from rice husk ash also did not have a biological cell toxicity effect [30].…”

Section: Discussionmentioning

confidence: 99%

In Vitro Degradation and Cytotoxicity of Eggshell-Based Hydroxyapatite: A Systematic Review and Meta-Analysis

et al. 2021

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Objective: This review focuses on the in vitro degradation of eggshell-based hydroxyapatite for analyzing the weight loss of hydroxyapatite when applied in the human body. Cytotoxicity tests were used to observe cell growth and morphological effects. A systematic review and meta-analysis were conducted to observe the weight loss and viable cells of hydroxyapatite when used for implants. Method: Based on the Population, Intervention, Comparison, and Outcome (PICO) strategy, the articles used for literature review were published in English on SCOPUS, PubMed, and Google Scholar from 1 January 2012 to 22 May 2021. Data regarding existing experiments in the literature articles the in vitro degradation and cytotoxicity testing of eggshell-based hydroxyapatite determined the biocompatibility of the materials. A meta-analysis was conducted to calculate the mean difference between the solutions and soaking times used for degradation and the stem cells used for cytotoxicity. Results: From 231 relevant studies, 71 were chosen for full-text analysis, out of which 33 articles met the inclusion criteria for degradation and cytotoxicity analysis. A manual search of the field of study resulted in three additional articles. Thus, 36 articles were included in this systematic review. Significance: The aim of this study was to highlight the importance of the biocompatibility of eggshell-based hydroxyapatite. The weight loss and viability cells of eggshell-based hydroxyapatite showed optimum results for viable cells requirements above 70%, and there is a weight loss of eggshell-based hydroxyapatite for a material implant. The meta-analysis indicated significant differences in the weight loss of eggshell-based hydroxyapatite materials with different soaking times and solutions used. The various kinds of stem cells for incubation of cultured cells in contact with a device, either directly or through diffusions with various kinds of stem cells from animals and humans, yielded viability cells above 70%.

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“…In the TGA profile of the α-TCP/GS sponge, there was no significant weight loss at high temperatures (above 600 • C); this could be explained by use of 60 mg α-TCP/GS (96.2 wt% of α-TCP content). Yilmaz et al [23] revealed that there is no significant change in the weight of hydroxyapatite even at high temperatures. Our findings indicated that α-TCP maintained its thermal stability.…”

Section: Discussionmentioning

confidence: 99%

Bone Regeneration by Dedifferentiated Fat Cells Using Composite Sponge of Alfa-Tricalcium Phosphate and Gelatin in a Rat Calvarial Defect Model

et al. 2021

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Mechanical and resorbable scaffolds are in high demand for stem cell-based regenerative medicine, to treat refractory bone defects in craniofacial abnormalities and injuries. Multipotent progenitor cells, such as dedifferentiated fat (DFAT) cells, are prospective sources for regenerative therapies. Herein, we aimed to demonstrate that a composite gelatin sponge (α-TCP/GS) of alfa-tricalcium phosphate (α-TCP) mixed with gelatin scaffolds (GS), with/without DFATs, induced bone regeneration in a rat calvarial defect model in vivo. α-TCP/GS was prepared by mixing α-TCP and 2% GS using vacuum-heated methods. α-TCP/GS samples with/without DFATs were transplanted into the model. After 4 weeks of implantation, the samples were subjected to micro-computed tomography (μ-CT) and histological analysis. α-TCP/GS possessed adequate mechanical strength; α-TCP did not convert to hydroxyapatite upon contact with water, as determined by X-ray diffraction. Moreover, stable α-TCP/GS was formed by electrostatic interactions, and verified based on the infrared peak shifts. μ-CT analyses showed that bone formation was higher in the α-TCP/GS+ DFAT group than in the α-TCP/GS group. Therefore, the implantation of α-TCP/GS comprising DFAT cells enhanced bone regeneration and vascularization, demonstrating the potential for healing critical-sized bone defects.

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Application of supercritical gel drying method on fabrication of mechanically improved and biologically safe three-component scaffold composed of graphene oxide/chitosan/hydroxyapatite and characterization studies

Cited by 29 publications

References 61 publications

Gradient Chitosan Hydrogels Modified with Graphene Derivatives and Hydroxyapatite: Physiochemical Properties and Initial Cytocompatibility Evaluation

Gradient Chitosan Hydrogels Modified with Graphene Derivatives and Hydroxyapatite: Physiochemical Properties and Initial Cytocompatibility Evaluation

In Vitro Degradation and Cytotoxicity of Eggshell-Based Hydroxyapatite: A Systematic Review and Meta-Analysis

Bone Regeneration by Dedifferentiated Fat Cells Using Composite Sponge of Alfa-Tricalcium Phosphate and Gelatin in a Rat Calvarial Defect Model

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