Application of ³¹P NMR spectroscopy to monitor chemotherapy‐associated changes of serum phospholipids in patients with malignant lymphomas

Abstract

31P spectra were obtained from 22 healthy volunteers and 35 patients with malignant lymphomas. Sera from patients were collected at the time of diagnosis and at several time-points during therapy. Long-term follow-up studies showed a good correlation between the 31P NMR spectra of sera and the clinically evident response of the disease to the chemotherapy. During therapy leading to remission resonance from phospholipids increased progressively resulting in spectra similar to those seen in normal sera. By contrast, in patients who did not respond to therapy the intensities of the phospholipid peaks remained relatively low or became progressively reduced as the disease progressed. To understand the source of the spectral differences, we also examined the concentrations of high-density lipoprotein, low-density lipoprotein, cholesterol, and triglycerides. In individuals responding to the treatment, both high-density lipoprotein and cholesterol increased to the point where they were statistically equivalent to those from healthy volunteers.

Metabonomic profiling of renal cell carcinoma: High-resolution proton nuclear magnetic resonance spectroscopy of human serum with multivariate data analysis

“…Alterations in blood lipid metabolism have been found in patients with thyroid [24] and other cancers [25,26]. The significant increase in the serum lipid concentration and concomitant decrease in the PCho/choline concentration we observed in RCC patients are consistent with those reported in an earlier study of RCC tissue [17].…”

Lipid changes occuring in the course of hematological cancers

“…In this study, however, the changes in the lipid fractions in these patients were less pronounced and limited to the HDL-C level. We previously documented an increase in HDL-C and TC levels after effective chemotherapy in patients with malignant lymphomas [17]. It appears reasonable that these lipid profile alterations could serve as one of the prognostic factors for an answer to treatment and remission.…”

31P NMR analysis of phospholipids in crude extracts from different sources: improved efficiency of the solvent system

“…As an example, the clinical analysis of serum PLs is a sensitive indicator in the monitoring of treatment in leukaemia. 9 The analysis of phospholipids from any kind of biological source requires a lipidic extraction10 followed by chemical analysis using chromatographic and/or spectroscopic techniques.11h14 The separation of the PL components from a mixture by thin-layer chromatography (TLC) or high-performance liquid chromatog-raphy (HPLC) followed by chemical analysis of the total phosphorus content or by spectrophotometric (UVÈvisible) or spectroÑuorimetric detection is required. These complicated procedures need chemical derivatizations and calibration using suitable internal standards.…”