31P NMR analysis of phospholipids in crude extracts from different sources: improved efficiency of the solvent system

Characterization of buffalo milk by 31P-nuclear magnetic resonance spectroscopy

“…We used as solvent a mixture of triethylamine/dimethylformamide/guanidinium hydrochloride (Bosco et al, 1997;Culeddu et al, 1998), which has been reported to overcome partition problems connected with the biphasic solvent and slightly enlarge the range of 31 P-NMR chemical shifts, thus improving the resolution. Furthermore, a lower chemical shift dependence on the phospholipid concentration is observed in this solution.…”

Analysis of phospholipid molecular species in brain by31P NMR spectroscopy

“…Branca et al (28) and Metz and Dunphy (19) describe a method for reliably obtaining a single-phase CHCl 3 -CH 3 OH-H 2 O solvent composition with reproducible and adequately resolved PL chemical shifts. Alternatively, another solvent system can be used (30).…”

Determination of Phospholipids in Olive Oil by 31P NMR Spectroscopy

“…Both factors result in significantly broad 31 P NMR resonances that prevent resolution and hence quantitation of the constituents phospholipids. In previous works, the formation of aggregates has been confronted by using detergents (16,19,26,27) or alternative mixtures of appropriate solvents (16,28). In the present study, the solvent effect on the 31 P NMR spectra profile was tested by dissolving the olive oil extracts in chloroform and in a mixture of CDCl 3 /MeOH/D 2 O-EDTA-Cs + (1.0 M) (400:80:5 v/v).…”

“…In the present study, the solvent effect on the 31 P NMR spectra profile was tested by dissolving the olive oil extracts in chloroform and in a mixture of CDCl 3 /MeOH/D 2 O-EDTA-Cs + (1.0 M) (400:80:5 v/v). The mixture of CDCl 3 / MeOH/ D 2 O-EDTA-Cs + was found most useful among several other solvents that were investigated (14,16). Figure 2 compares the spectra of phospholipids extracted from a virgin olive oil sample of KORONEIKI cultivar originating from Heraklion in chloroform and CDCl 3 /MeOH/D 2 O-EDTA-Cs solvents.…”

“…As phospholipids give a single signal in the spectrum, and different phospholipids are characterized by specific resonance frequencies (due to different chemical structures of the phospholipids), separation of the mixture components is not necessary to analyze it. 31 P NMR spectroscopy has been used to quantitate phospholipids from different sources, including lecithins (14)(15)(16)(17), lipids (18,19), and biological fluids and tissues (17)(18)(19)(20)(21)(22)(23)(24). A comparison study (17) for the determination of phospholipids in lecithins has shown that TLC, HPLC, and 31 P NMR methods gave comparable results concerning the total amounts of phospholipids, as well as the amounts of phospholipids classes in the samples used.…”