Application of statistical experimental design for optimization of downstream process for recovery of pullulan produced by Aureobasidium pullulans HP-2001

Abstract

The optimal conditions of the downstream process for recovery of pullulan produced by Aureobasidium pullulans HP-2001 were examined using response surface method (RSM). The optimal amount of diatomite in filter press and the optimal flow rate in a continuous flow centrifuge for removal of cells from the culture broth of A. pullulans HP-2001 were found to be 5.0% (v/v) and 2.0 L/min. Based on central composite design (CCD) experiments and analysis of variance (ANOVA) indicated that the optimal conditions for recovery of pullulan from the supernatant by precipitation were the volume ratio of ethanol (or isopropanol) to supernatant of 3.0 : 1.0, the reaction time of 29.5 h, and the reaction temperature of 20.2 o C. The expected maximal recovery yields of pullulan using ethanol and isopropanol under optimized conditions were 79.2 and 85.5%, respectively.

Development of Improved Process with Treatment of Cellulase for Isolation of Ampelopsin from Dried Fruits of Ampelopsis grossedentata

“…It had two chambers; the filter area and volume of each chamber were 0.11 m 2 and 1.7 L (Kim et al 2011). A continuous flow centrifuge (Tubular Type Centrifuge, Kokusan Chemical Co. Ltd., Tokyo, Japan) was used for removal of insoluble materials after hydrolysis.…”