Application of Reverse Genetics in Functional Genomics of Potyvirus

Kannan, Maathavi; Zainal, Zamri; Ismail, Ismanizan; Baharum, Syarul Nataqain; Bunawan, Hamidun

doi:10.3390/v12080803

Cited by 4 publications

(3 citation statements)

References 100 publications

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“…Full-length infectious clones are universal, indispensable tools for virus biology research and the development of experimental systems for investigating diseases (Pasin, Menzel and Daròs 2019 , Kannan et al . 2020 ). They have been generated for members of Potyvirus (Domier et al .…”

Section: Biotech Appeal Of Non-core Modulesmentioning

confidence: 99%

Proteome expansion in thePotyviridaeevolutionary radiation

Pasin

Daròs

Tzanetakis

2022

FEMS Microbiology Reviews

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Potyviridae, the largest family of known RNA viruses (realm Riboviria), belongs to the picorna-like supergroup and has important agricultural and ecological impacts. Potyvirid genomes are translated into polyproteins, which are in turn hydrolyzed to release mature products. Recent sequencing efforts revealed an unprecedented number of potyvirids with a rich variability in gene content and genomic layouts. Here we review the heterogeneity of non-core modules that expand the structural and functional diversity of the potyvirid proteomes. We provide a family-wide classification of P1 proteinases into the functional Types A and B, and discuss pretty interesting sweet potato potyviral ORF (PISPO), putative zinc fingers, and alkylation B (AlkB) – non-core modules found within P1 cistrons. The atypical modules inosine triphosphate pyrophosphatase (ITPase/HAM1), as well as the pseudo tobacco mosaic virus-like coat protein (TMV-like CP) are discussed alongside homologs of unrelated virus taxa. Family-wide abundance of the multitasking helper component proteinase (HC-pro) is revised. Functional connections between non-core modules are highlighted to support host niche adaptation and immune evasion as main drivers of the Potyviridae evolutionary radiation. Potential biotechnological and synthetic biology applications of potyvirid leader proteinases and non-core modules are finally explored.

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Section: Biotech Appeal Of Non-core Modulesmentioning

confidence: 99%

Proteome expansion in thePotyviridaeevolutionary radiation

Pasin

Daròs

Tzanetakis

2022

FEMS Microbiology Reviews

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“…Potyviruses exist as flexuous rod-shaped particles ranging from 720 to 900 nm in length with an ssRNA genome of approximately 10 kb. Potyviruses have only an ORF encoding a large polyprotein, which is processed into ten functional proteins by three viral proteinases [53]. P3N-PIPO protein is produced using a viral polymerase slippage mechanism [54].…”

Section: Potyvirusesmentioning

confidence: 99%

Plant Virus-Based Tools for Studying the Function of Gene and Noncoding RNA in Cucurbits

Zhou,

Li,

Geng

2023

Biological and Abiotic Stress in Cucurbitaceae Crops

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Cucurbits are economically important crops worldwide. The genomic data of many cucurbits are now available. However, functional analyses of cucurbit genes and noncoding RNAs have been impeded because genetic transformation is difficult in many cucurbitaceous plants. The cucurbits-infecting plant viruses can be modified into useful tools for functional genomic studies in cucurbits, which provide alternative ways for rapid characterization of gene and noncoding RNA functions. This review will focus on the advancement and application of plant viruses-based gene silencing, gene expressing, and noncoding RNA regulation tools for studying the development, fruits, and stress of cucurbits. The features, advantages, and disadvantages of different plant virus vectors will be discussed in detail. We hope this review will provide guidance for studies on cucurbitaceous plants.

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“…To characterise a virus at the molecular level, constructing an infectious clone is necessary to study viral replication, range of host species, movement, pathogenicity, and genomic regions' roles [4]. However, the construction of an infectious clone that involves the cloning of many subgenomic cDNA fragments is frequently laborious [5]. The construction of an infectious clone is still gruelling and many obstacles remain including unpredictability and toxicity in bacterial host-virus sequences [6,7].…”

Section: Introductionmentioning

confidence: 99%

Construction of Infectious Clones of Begomoviruses: Strategies, Techniques and Applications

Saad

Akbar

et al. 2021

Biology

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Begomovirus has become a potential threat to the agriculture sector. It causes significant losses to several economically important crops. Given this considerable loss, the development of tools to study viral genomes and function is needed. Infectious clones approaches and applications have allowed the direct exploitation of virus genomes. Infectious clones of DNA viruses are the critical instrument for functional characterization of the notable and newly discovered virus. Understanding of structure and composition of viruses has contributed to the evolution of molecular plant pathology. Therefore, this review provides extensive guidelines on the strategy to construct infectious clones of Begomovirus. Also, this technique’s impacts and benefits in controlling and understanding the Begomovirus infection will be discussed.

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Application of Reverse Genetics in Functional Genomics of Potyvirus

Cited by 4 publications

References 100 publications

Proteome expansion in thePotyviridaeevolutionary radiation

Proteome expansion in thePotyviridaeevolutionary radiation

Plant Virus-Based Tools for Studying the Function of Gene and Noncoding RNA in Cucurbits

Construction of Infectious Clones of Begomoviruses: Strategies, Techniques and Applications

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