Construction of Infectious Clones of Begomoviruses: Strategies, Techniques and Applications

Saad, Mohd Zuwairi Mat; Sau, Aziz Ramlee; Akbar, Muhamad Afiq; Baharum, Syarul Nataqain; Ramzi, Ahmad Bazli; Talip, Noraini; Bunawan, Hamidun

doi:10.3390/biology10070604

Cited by 10 publications

(3 citation statements)

References 135 publications

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“…Furthermore, the infectious viral DNAs can also be applied in determining the virulence of viral DNAs, screening virus resistance, studying viral gene function and virus-vector-host interaction, etc. [ 52 , 53 , 54 ].…”

Section: Discussionmentioning

confidence: 99%

Genetic Diversity and Geographic Distribution of Cucurbit-Infecting Begomoviruses in the Philippines

Neoh

Lai

Lin

et al. 2023

Plants

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Cucurbits are important economic crops worldwide. However, the cucurbit leaf curl disease (CuLCD), caused by whitefly-transmitted begomoviruses constrains their production. In Southeast Asia, three major begomoviruses, Tomato leaf curl New Delhi virus (ToLCNDV), Squash leaf curl China virus (SLCCNV) and Squash leaf curl Philippines virus (SLCuPV) are associated with CuLCD. SLCuPV and SLCCNV were identified in Luzon, the Philippines. Here, the genetic diversity and geographic distribution of CuLCD-associated begomoviruses in the Philippines were studied based on 103 begomovirus detected out of 249 cucurbit samples collected from 60 locations throughout the country in 2018 and 2019. The presence of SLCCNV and SLCuPV throughout the Philippines were confirmed by begomovirus PCR detection and viral DNA sequence analysis. SLCuPV was determined as a predominant CuLCD-associated begomovirus and grouped into two strains. Interestingly, SLCCNV was detected in pumpkin and bottle gourd without associated viral DNA-B and mixed-infected with SLCuPV. Furthermore, the pathogenicity of selected isolates of SLCCNV and SLCuPV was confirmed. The results provide virus genetic diversity associated with CuLCD for further disease management, especially in developing the disease-resistant cultivars in the Philippines as well as Southeast Asia.

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Section: Discussionmentioning

confidence: 99%

Genetic Diversity and Geographic Distribution of Cucurbit-Infecting Begomoviruses in the Philippines

Neoh

Lai

Lin

et al. 2023

Plants

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“…Begomovirus menyerang berbagai jenis tanaman dikotil serta merupakan kelompok virus penyebab penyakit yang penting pada banyak komoditas hortikultura di berbagai daerah tropis maupun subtropis (Saunders et al, 2000;Saad et al, 2021).…”

Section: Pendahuluanunclassified

Identifikasi Virus Penyebab Penyakit Kuning Keriting pada Cabai di Kabupaten Gowa, Sulawesi Selatan

Hamdayanty,

Hardina

2023

Agrikultura

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Cabai merah merupakan salah satu komoditas sayuran yang banyak digemari oleh masyarakat Indonesia baik sebagai bumbu dapur maupun pelengkap makanan. Tingkat produksi cabai merah masih berfluktuasi. Salah satu penyebabnya yakni adanya serangan Pepper yellow leaf curl virus (PepYLCV). Tujuan penelitian ini adalah mengidentifikasi secara molekuler penyakit PepYLCV dan melaporkan keberadaan penyakit tersebut pada pertanaman cabai di Kabupaten Gowa, Sulawesi Selatan. Pengambilan sampel tanaman cabai dilakukan di tiga kecamatan berbeda di Kabupaten Gowa dimana cabai banyak ditanam. Sampel yang diambil selanjutnya diekstraksi yang dilanjutkan dengan proses PCR, sekuen nukleotida, dan analisis filogenetika. Gejala infeksi virus pada tanaman cabai yang ditemukan pada tiga kecamatan di Kabupaten Gowa adalah mosaik kuning dan hijau, daun menggulung, melengkung ke atas dan/atau ke bawah, dan tanaman kerdil. Keparahan penyakit dan kejadian penyakit PepYLCV di Kabupaten Gowa masing-masing 78% dan 44%. Gejala infeksi virus pada tanaman cabai positif terinfeksi Begomovirus dan hasil analisis sekuensing menunjukkan bahwa virus tersebut adalah PepYLCV. PepYLCV di Kabupaten Gowa memiliki kekerabatan yang dekat dengan PepYLCV Bali. Hasil penelitian ini merupakan laporan pertama keberadaan PepYLCV di Sulawesi Selatan.

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“…Begomoviruses, which constitute the largest genera within Geminiviridae are transmitted by B. tabaci. The genomic size of begomoviruses (monopartite or bipartite) ranges from 2.7 to 3.0 kb (Figure 1) [16]. The DNA-A of the bipartite begomoviruses comprises six incomplete open reading frames (ORFs), which encode various proteins, including AC1/Rep, AC2/TrAP, AC3/REn, AV2, AV1/CP, and AC4 proteins [17,18].…”

Section: Introductionmentioning

confidence: 99%

A Review of Interactions between Plants and Whitefly-Transmitted Begomoviruses

Naveed,

Islam,

Jafir

et al. 2023

Plants

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The transmission of plant viruses from infected to healthy host plants is a process in which insects play a major role, using various transmission strategies. Environmental factors have an impact on the transmission of viruses and the subsequent development of infections or diseases. When viruses are successful, plant virus diseases can reach epidemic proportions. Many plants across different regions are vulnerable to viral infections transmitted by the whitefly vector. Begomoviruses, which are transmitted by whiteflies, represent a significant threat to agriculture worldwide. The review highlights the mechanisms of virus acquisition and transmission by whiteflies and explores the factors influencing these interactions. Understanding the impacts of these changes is crucial for managing the spread of pests and mitigating damage to crops. It underscores the need for continued research to elucidate the mechanisms driving plant–insect–virus interactions and to identify new approaches for sustainable pest management.

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Construction of Infectious Clones of Begomoviruses: Strategies, Techniques and Applications

Cited by 10 publications

References 135 publications

Genetic Diversity and Geographic Distribution of Cucurbit-Infecting Begomoviruses in the Philippines

Genetic Diversity and Geographic Distribution of Cucurbit-Infecting Begomoviruses in the Philippines

Identifikasi Virus Penyebab Penyakit Kuning Keriting pada Cabai di Kabupaten Gowa, Sulawesi Selatan

A Review of Interactions between Plants and Whitefly-Transmitted Begomoviruses

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