Application of response surface methodology to optimize the production of antimicrobial metabolites by <i>Micromonospora</i> Y15

Wang, Liping; Zhang, Meng; Li, Yuntao; Cui, Yunyun; Zhang, Yu; Wang, Zhengquan; Wang, Mingfu; Huang, Yuliang

doi:10.1080/13102818.2017.1356689

Cited by 15 publications

(12 citation statements)

References 34 publications

(52 reference statements)

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“…In addition, RSM has been used to optimize antimicrobial metabolite production by up to 3.86-fold, in Micromonospora sp. Y15 a sea mud isolate from the South China Sea [ 149 ]. With respect to marine fungi, RSM has also been employed to optimize culture conditions for the production of penicilazaphilone C, a new antineoplastic and azaphilone from the marine-derived fungus Penicillium sclerotiorum M-22 [ 150 ].…”

Section: High Throughput Methods To Streamline Cultivation Based Bmentioning

confidence: 99%

Extending the “One Strain Many Compounds” (OSMAC) Principle to Marine Microorganisms

et al. 2018

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Genomic data often highlights an inconsistency between the number of gene clusters identified using bioinformatic approaches as potentially producing secondary metabolites and the actual number of chemically characterized secondary metabolites produced by any given microorganism. Such gene clusters are generally considered as “silent”, meaning that they are not expressed under laboratory conditions. Triggering expression of these “silent” clusters could result in unlocking the chemical diversity they control, allowing the discovery of novel molecules of both medical and biotechnological interest. Therefore, both genetic and cultivation-based techniques have been developed aimed at stimulating expression of these “silent” genes. The principles behind the cultivation based approaches have been conceptualized in the “one strain many compounds” (OSMAC) framework, which underlines how a single strain can produce different molecules when grown under different environmental conditions. Parameters such as, nutrient content, temperature, and rate of aeration can be easily changed, altering the global physiology of a microbial strain and in turn significantly affecting its secondary metabolism. As a direct extension of such approaches, co-cultivation strategies and the addition of chemical elicitors have also been used as cues to activate “silent” clusters. In this review, we aim to provide a focused and comprehensive overview of these strategies as they pertain to marine microbes. Moreover, we underline how changes in some parameters which have provided important results in terrestrial microbes, but which have rarely been considered in marine microorganisms, may represent additional strategies to awaken “silent” gene clusters in marine microbes. Unfortunately, the empirical nature of the OSMAC approach forces scientists to perform extensive laboratory experiments. Nevertheless, we believe that some computation and experimental based techniques which are used in other disciplines, and which we discuss; could be effectively employed to help streamline the OSMAC based approaches. We believe that natural products discovery in marine microorganisms would be greatly aided through the integration of basic microbiological approaches, computational methods, and technological innovations, thereby helping unearth much of the as yet untapped potential of these microorganisms.

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Section: High Throughput Methods To Streamline Cultivation Based Bmentioning

confidence: 99%

Extending the “One Strain Many Compounds” (OSMAC) Principle to Marine Microorganisms

et al. 2018

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“…Table 4 shows the design and obtained experimental responses from PBD and the runs produced considerably improved results for kalafungin production. The effects of extraction parameters and production of antimicrobial metabolites from Micromonospora and relative yield was assessed by a three-level threefactor Box-Behnken design and reported [37]. Yang et al optimized the effective culture conditions of amoxicillin degrading bacteria using RSM [38].…”

Section: Media Optimization For Culture Conditions By Box-behnken Designmentioning

confidence: 99%

Statistical optimization of fermentation media for beta lactamase inhibitor kalafungin production from marine Streptomyces sp. SBRK1

Rajam¹,

Kannan²,

Muthamil³

et al. 2021

J App Biol Biotech

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β-lactamase inhibitors are potent synergistic drugs to reverse the drug resistant populations to sensitive ones. The production of such commercially important compounds needs much consideration on its production from the source organism. In the present study, a marine Streptomyces derived β-lactamase inhibitory compound kalafungin was optimized under solid state fermentation conditions. This study intended to facilitate the production of β-lactamase inhibitor by applying statistical methods of optimizing the culturing process variables for secondary metabolite biosynthesis. The initial optimization revealed starch as a main carbon source played a vital role in kalafungin production at 0.1% NaCl concentration by one-factor-at-a-time method. Further it was carried forward to maximize the productivity of kalafungin through fractional factorial design and the optimum medium composition by Response Surface Methodology (RSM). Plackett-Burman design and Box-Behnken design experiments have given the maximum production of 51.4 mg/L of kalafungin at 29°C for 8 days of incubation which is 25.06% more than the unoptimized conditions. The experimental values are closer to predicted values and it has strongly supported the use of RSM in fermentation condition optimization for kalafungin production.

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“…Carbon sources are essential components in the culture media. Several reports showed that the optimum antimicrobial agent production depends upon the type and concentration of carbon sources used in culture media such as (starch, glucose, maltose, fructose, glycerol, and molasses) (Amin et al, 2017a;Amin et al, 2017b;Pharm, 2010;Taurino et al, 2011;Wang et al, 2017;Abdelwahed et al, 2012). Also, the type and amount of nitrogen sources such as ammonium sulfate, ammonium nitrate, ammonium chloride, peptone, soya bean meal, and yeast extract greatly influence the antimicrobial production by Actinobacteria (Amin et al, 2017a;Amin et al, 2017b;Ahmad et al, 2017;Taurino et al, 2011;Wang et al, 2017;Abdelwahed et al, 2012).…”

Section: Factors Affecting Antibiotic Productionmentioning

confidence: 99%

Microbiological and molecular insights on rare Actinobacteria harboring bioactive prospective

et al. 2020

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Background: Actinobacteria is as a group of advanced filamentous bacteria. Rare Actinobacteria are of special interest as they are rarely isolated from the environments. They are a major source of important bioactive compounds. Determining the proper strategy for the identification of Actinobacteria harboring biosynthetic gene clusters and producing bioactive molecules is a challenging platform. Methodology: In this review, we discuss a consequence of microbiological and molecular methods for the identification of rare Actinobacteria. In addition to that, we shed light on rare Actinobacteria's significance in antibiotic production. We also clarified molecular approaches for the manipulation of novel biosynthetic gene clusters via PCR screening, fosmid libraries, and Illumina whole-genome sequencing in combination with bioinformatics analysis. Conclusion: Perceptions of the conventional and molecular identification of Actinobacteria were conducted. This will open the door for the genetic manipulation of novel antibiotic gene clusters in heterologous hosts. Also, these conclusions will lead to constructing new bioactive molecules via genetically engineering biosynthetic pathways.

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Application of response surface methodology to optimize the production of antimicrobial metabolites by Micromonospora Y15

Cited by 15 publications

References 34 publications

Extending the “One Strain Many Compounds” (OSMAC) Principle to Marine Microorganisms

Extending the “One Strain Many Compounds” (OSMAC) Principle to Marine Microorganisms

Statistical optimization of fermentation media for beta lactamase inhibitor kalafungin production from marine Streptomyces sp. SBRK1

Microbiological and molecular insights on rare Actinobacteria harboring bioactive prospective

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