Application of Response Surface Methodology for Optimizing the Therapeutic Activity of ZnO Nanoparticles Biosynthesized from Aspergillus niger

Abstract

In this study, the biosynthesis of zinc oxide nanoparticles using Aspergillus niger (A/ZnO-NPs) is described. These particles have been characterized by UV–Vis spectrum analysis, x-ray powder diffraction, field emission scanning electron microscopy, and transmission electron microscopy. To use this biosynthesized nanoparticle as an antiproliferative and antimicrobial agent, the IC50 value against the breast cancer cell line and inhibition zone against Escherichia coli were used to optimize the effect of two processing factors including dose of filtrate fungi cell and temperature. The biosynthesized A/ZnO-NPs had an absorbance band at 320 nm and spherical shapes. The mean particles size was 35 nm. RSM (response surface methodology) was utilized to investigate the outcome responses. The Model F-value of 12.21 and 7.29 implies that the model was significant for both responses. The contour plot against inhibition zone for temperature and dose showed that if the dose increases from 3.8 to 17.2 µg/mL, the inhibition zone increases up to 35 mm. As an alternative to chemical and/or physical methods, biosynthesizing zinc oxide NPs through fungi extracts can serve as a more facile and eco-friendly strategy. Additionally, for optimization of the processes, the outcome responses in the biomedical available test can be used in the synthesis of ZnO-NPs that are utilized for large-scale production in various medical applications.

Collagen Hydrogels Loaded with Silver Nanoparticles and Cannabis Sativa Oil

“…For this, a capping agent was used to prevent agglomeration and stabilize the nanoparticles in the aqueous suspension. Although natural polymers have been demonstrated to have plenty of advantages for pharmacological and bionanomedical applications [ 26 , 27 ], plant-based compounds involve time-consuming extraction processes and may have microbial contamination. In contrast, synthetic polymers have great advantages over natural polymers, since they can be adapted to offer a wide range of possibilities with different kinds of products [ 28 ].…”

Green Nanotechnology: Recent Research on Bioresource-Based Nanoparticle Synthesis and Applications

“…As an alternative to chemical and/or physical approaches, biosynthesizing zinc oxide NPs using fungal extracts may be a more e cient and environmentally friendly solution. Furthermore, for process optimization, the results of the biomedical available test may be employed in the synthesis of ZnO NPs, which are used for large-scale fabrication in a variety of medical applications [59].…”

Resveratrol-Mediated Gold-Nanoceria Synthesis as Green Nanomedicine for Phytotherapy of Hepatocellular Carcinoma

“…Nanotechnology-mediated delivery can be employed as a beneficial device in increasing the bioavailability of resveratrol. Nanoscience is an emerging part of investigation that operates at the crossways of biology, physico- chemicals, engineering, pharmacology, and medicine [22,[46][47][48]. Designing of NPs (nano particles) for an effective and controlled delivery of anticancer factors is being considered as one of the most important applications of nanoscience [24,49,50].…”